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  • Oxford University Press  (40)
  • BioMed Central  (32)
  • Nature Publishing Group
  • 2020-2022  (15)
  • 2010-2014  (57)
  • 1975-1979  (2)
  • 1955-1959  (5)
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  • 1
    facet.materialart.
    Unbekannt
    Thermodynamic analysis of DNA binding by a Bacillus single stranded DNA binding protein (2012)
    BioMed Central
    Details
    Publikationsdatum: 2012-06-15
    Beschreibung: Background: Single-stranded DNA binding proteins (SSB) are essential for DNA replication, repair, and recombination in all organisms. SSB works in concert with a variety of DNA metabolizing enzymes such as DNA polymerase. Results: We have cloned and purified SSB from Bacillus anthracis (SSBBA). In the absence of DNA, at concentrations [less than or equal to]100 mug/ml, SSBBA did not form a stable tetramer and appeared to resemble bacteriophage T4 gene 32 protein. Fluorescence anisotropy studies demonstrated that SSBBA bound ssDNA with high affinity comparable to other prokaryotic SSBs. Thermodynamic analysis indicated both hydrophobic and ionic contributions to ssDNA binding. FRET analysis of oligo(dT)70 binding suggested that SSBBA forms a tetrameric assembly upon ssDNA binding. This report provides evidence of a bacterial SSB that utilizes a novel mechanism for DNA binding through the formation of a transient tetrameric structure. Conclusions: Unlike other prokaryotic SSB proteins, SSBBA from Bacillus anthracis appeared to be monomeric at concentrations [less than or equal to]100 mug/ml as determined by SE-HPLC. SSBBA retained its ability to bind ssDNA with very high affinity, comparable to SSB proteins which are tetrameric. In the presence of a long ssDNA template, SSBBA appears to form a transient tetrameric structure. Its unique structure appears to be due to the cumulative effect of multiple key amino acid changes in its sequence during evolution, leading to perturbation of stable dimer and tetramer formation. The structural features of SSBBA could promote facile assembly and disassembly of the protein-DNA complex required in processes such as DNA replication.
    Digitale ISSN: 1471-2091
    Thema: Chemie und Pharmazie
    Publiziert von BioMed Central
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
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    Thermodynamic analysis of DNA binding by a Bacillus single stranded DNA binding protein (2012)
    BioMed Central
    Details
    Publikationsdatum: 2012-01-01
    Digitale ISSN: 1471-2091
    Thema: Chemie und Pharmazie
    Publiziert von BioMed Central
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Protection of spermatogenesis by α 2u globulin in rats treated with oestrogen (1976)
    [s.l.] : Nature Publishing Group
    Nature 260 (1976), S. 719-721 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] α2u Globulin was prepared from male rat urine as before7. The protein was purified further on a Sephadex G-75 column. α2u Globulin prepared according to the above procedure gave a single electrophoretic band on sodium dodecyl sulphate(SDS)?polyacrylamide gel electrophoresis. Because ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/260719a0
    Standort Signatur Erwartet Verfügbarkeit
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    Volltext
  • 4
    Digitale Medien
    Digitale Medien
    Enhanced abundances of Ca, Ti and Cr in low energy cosmic rays in Skylab experiment (1975)
    [s.l.] : Nature Publishing Group
    Nature 258 (1975), S. 409-412 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] The detector consisted of a stack of 32 lexan polycarbonate sheets, each of area 180cm2 and thickness 0.025 cm, covered with aluminium foil of a minimum thickness of 27 mgcm2. This module was exposed on the exterior of Skylab III at an altitude of 435 km in a circular orbit of inclination 50 for 73 ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/258409a0
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Chemical Nature of Nucleic Acids in Cyanophyceae (1956)
    [s.l.] : Nature Publishing Group
    Nature 177 (1956), S. 95-96 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] The deoxyribonucleic acid separated from Nostoc muscorum gives a positive test with the Dische reagent, indicating the presence of deoxypentose-sugar. Chromatographic analysis of the hydrolysate according to the procedure of Wyatt2 revealed only three spots of bases, corresponding to guanine, ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/177095b0
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Slow Disappearance of Fluorescence in Thionine on Addition of Abietic Acid (1955)
    [s.l.] : Nature Publishing Group
    Nature 176 (1955), S. 214-215 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] It can be seen from Fig. 1 (curves 1, 2 and 3) that the higher the concentration of the acid or the dye, the more rapid is the quenching. Curve 4 shows that a higher temperature brings about faster quenching, and curve 5 shows that the medium strongly influences the process, n-butyl alcohol ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/176214a0
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  • 7
    Digitale Medien
    Digitale Medien
    Surface-active Characteristics of Sodium Anacardate isolated from Cashew Nut Shell Oil (1958)
    [s.l.] : Nature Publishing Group
    Nature 182 (1958), S. 1299-1300 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] Shell liquid extracted with 95 per cent ethyl alcohol was saponified and the non-saponifiable material was removed by extraction with ethyl ether. Stage-wise liberation of cardol and then of anacardic acid (followed by separate extractions with ethyl ether), was carried out by passing carbon ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/1821299b0
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Thioctic Acid and Photosynthetic Fixation of Carbon Dioxide (1958)
    [s.l.] : Nature Publishing Group
    Nature 181 (1958), S. 1219-1220 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] Seven-day old cultures of Chlorella pyrenoidosa and Sce ±edesmus bliquus were centrifuged down at 12 C. in an MSE centrifuge and resuspended in 10 ml. phosphate buffer of pH. 6-8. The cells were then incubated with different concentrations of thioctic acid and 30 JJLC. of sodium ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/1811219a0
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    Relationship between Auxins and Nucleic Acid Synthesis in Coleoptile Tissues (1959)
    [s.l.] : Nature Publishing Group
    Nature 183 (1959), S. 1824-1825 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] Seeds of Avena sativa var. N.P.I and Oryza sativa var. Rupsail were used. The seeds were germinated in the dark with occasional red light for 3 days. The coleoptiles were then decapitated at 2-3 mm. and floated on water for 2 hr. to free them from any endogenous auxin. After a further decapitation ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/1831824b0
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  • 10
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    Invaders' control on post-disturbance succession in coastal mangroves (2012)
    Oxford University Press
    Details
    Publikationsdatum: 2012-04-13
    Beschreibung: Aims In recent years, coastal mangroves have been frequently affected by large disturbances (cyclones, hurricanes, flooding and tsunamis) and post-disturbance vegetation is often dominated by small stature mangrove, mangrove-associate and non-mangrove species potentially affecting ecosystem functioning. Knowledge on the processes of mangrove vegetation development and recovery (succession) following normal and large disturbances will benefit practitioners in designing robust ecosystem management/restoration plans. Here we propose a conceptual model of disturbance-mediated succession in mangroves. Methods Based on field observations and species’ life history traits, we develop conceptual models of mangrove succession under normal disturbance regime and recently experienced increased frequency of large disturbances. We evaluate our conceptual models by conducting a scenario testing experiment. Important Findings We suggest two predominant processes affecting mangrove succession after disturbance: propagule limitation due to damage of seed producing mature trees and dispersal barrier resulting from biological invasion associated with large disturbance. We argue that large disturbances affect mature trees more than the small-stature non-tree (shrubs, herbs and climbers) species creating a larger propagule shortage for mangrove tree species than non-tree species. Secondly, large disturbances facilitate invasion of free-floating aquatics, which may interfere with the flow-facilitated propagule dispersal and seedling establishment of mangrove species. In a scenario testing experiment, we have shown that similar levels of disturbance impact vegetation development and recovery differently depending on the presence or absence of invasive species. We conclude that since biological invasion is one of the major drivers of post-disturbance mangrove succession, the dimension of biological invasion should be included in prediction, management and restoration of mangrove forests.
    Print ISSN: 1752-993X
    Digitale ISSN: 1752-9921
    Thema: Biologie
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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