ISSN:
1617-4623
Keywords:
Protoplast culture
;
Nuclear male sterility
;
Mitochondrial DNA recombination
;
Substoichiometric molecules
;
Nicotiana sylvestris
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary ANicotiana sylvestris plant regenerated from protoplast culture was found to be mutated in both the mitochondrial (mt) and nuclear genomes. The novel mt DNA organization, called U, is due to the amplification of recombinant substoichiometric DNA sequences that preexist in the parent line. The recombination event involves two 404 by repeats, which hybridize to a 2.1 kb transcript. Although the sequence of both repeats was not altered by the recombination, an additional transcript of 2.5 kb was detected in U mitochondria. In addition to this mitochondrial reorganization, the protoclone carried a recessive nuclear mutation conferring male sterility (ms4). A possible role ofms4 in the appearance of the U mt DNA organization was investigated by introducing this gene into normalN. sylvestris cytoplasm. No mt DNA change could be found in homozygousms4/ms4 plants of the F2 generation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00587579
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