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  • Ascites hepatoma  (1)
  • Curve fit  (1)
  • Springer  (2)
  • Blackwell Publishing Ltd
  • Cell Press
  • 2020-2022
  • 2010-2014
  • 1990-1994  (2)
  • 1935-1939
Collection
Keywords
Publisher
  • Springer  (2)
  • Blackwell Publishing Ltd
  • Cell Press
Years
  • 2020-2022
  • 2010-2014
  • 1990-1994  (2)
  • 1935-1939
Year
  • 1
    Electronic Resource
    Electronic Resource
    A monoclonal antibody disrupting cell-cell adhesion of rat ascites hepatoma cells (1993)
    Springer
    Cell & tissue research 273 (1993), S. 363-370 
    Details
    ISSN: 1432-0878
    Keywords: Ascites hepatoma ; Adhesive factor ; Monoclonal antibody ; Cell membrane polarity ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A cell surface-associated adhesive factor (AF) separated from differentiated rat ascites hepatoma AH136B cells (forming cell islands in vivo) has been highly purified by chromatography. AF is assumed to mediate the cell-cell adhesion essential to island formation of the hepatoma cells. A substance, immunologically crossreactive with AF, is present in the ascites fluid or culture medium of the AH136B cells. Because the substance is almost identical to AF in molecular weight and aggregation-promoting activity, it has been concluded that AF is released into the ascites fluid where it is concentrated. Monoclonal antibodies have been raised against AF purified from ascites fluid of AH136B cells. We have obtained a monoclonal antibody, coded MoAF-6D6, that strongly abolishes the aggregation-promoting activity of AF. When AH136B cell islands are incubated in the presence of Fab fragments of MoAF-6D6, cell detachment from the islands is evident within 24 h. Cell islands following 36-h culture show a distinct dissociation and islands completely lose their organization 48 h after culture. The dissociating effect of MoAF-6D6 is neutralized by the addition of AF. These results suggest that AF plays a significant role in the maintenance of cell islands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312839
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  • 2
    Electronic Resource
    Electronic Resource
    Estimation of left ventricular myocardial elasticity and viscosity by a thick-walled spherical model (1993)
    Springer
    Medical & biological engineering & computing 31 (1993), S. 325-332 
    Details
    ISSN: 1741-0444
    Keywords: Curve fit ; Finite-element method ; Left ventricle ; Thick-walled spherical shell ; Transfer function ; Viscoelastic properties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The authors measured the transfer function (TF) of the left ventricle (LV) in an isolated canine preparation. Here TF indicates the ratio of induced vibration in LV to input vibration when an external mechanical oscillation is applied. TF had a single peak the frequency of which changed from 40 Hz to 80 Hz when LV pressure (LVP) increased from 6 mm Hg to 96 mm Hg. A mathematical model was formulated to estimate the viscoelasticity of the spherical shell. This model was constructed of the material points, elastic components which connected all the material points, and viscous components placed in series with elastic components. Theoretical TF can be computed if the viscoelastic values are given. The value of viscoelasticity at which the theoretical TF best fitted the experimental TF was considered to be the viscoelasticity of the model. The validity of this approach was verified using a silicone spherical shell. The estimated myocardial elasticity was 40 kPa when LVP was 6 mm Hg, 160–170 kPa when LVP was 96 mm Hg and was approximately proportional to LVP, whereas viscosity showed small change. The inclination of elasticity was consistent with previous reports. These results proved that myocardial elasticity can be estimated by analysing the transfer function of the left ventricle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02446683
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    Paper (German National Licenses)
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