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The gene for the α-subunit of ATPase: a site of homologous recombination in plant mitochondrial DNA also functions in somatic hybrid cells (1992)

Honda, H. ; Hirai, A.

Springer

Theoretical and applied genetics 84 (1992), S. 33-38

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ISSN: 1432-2242

Keywords: Homologous recombination ; ATPase α-subunit ; Protoplast fusion ; Nicotiana

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Segments of mitochondrial DNA (mtDNA) carrying the gene for the α-subunit of F1-ATPase (atpA) were detected by Southern hybridization with atpA from pea as probe. In the case of Nicotiana langsdorffii, we identified four fragments that are derived from combinations of two different 5′ and two different 3′ flanking regions of atpA. All four types share the coding region, suggesting that they result from homologous recombination in the coding region of atpA. By contrast, N. glauca generated only one analogous fragment, which indicated the existence of only a single type of atpA in N. glauca. In the case of somatic hybrids obtained by fusion between protoplasts from N. langsdorffii and N. glauca, analysis with EcoRI or HindIII detected three new fragments in addition to the parental fragments. These new fragments can be explained by homologous recombination within the coding region of atpA. Our results show that the coding region of atpA is involved not only in intragenomic homologous recombination but can also be involved in homologous recombination between two parental mitochondrial genomes of somatic hybrids.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00223978

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Isolation of the intermetallic compounds Al2Cu, Al12Mg17 Al3Fe, Al9Co2 and Al3Ni in binary aluminium alloys by application of an organic solvent (1972)

Honda, F. ; Hirokawa, K.

Springer

Fresenius' Zeitschrift für analytische Chemie 262 (1972), S. 170-179

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ISSN: 1618-2650

Keywords: Isolierung intermetallischer Verbindungen von Aluminium mit Kupfer, Magnesium, Eisen, Kobalt oder Nickel aus binären Aluminiumlegierungen ; Behandlung mit organ. Lösungsmittel

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Description / Table of Contents: Summary For the isolation of the intermetallic compounds Al2Cu, Al12Mg17, Al3Fe, Al9Co2 and Al3Ni in their binary alloys a new method is proposed: Sample alloy particles are placed in a flask with a methanolic solution of benzoic acid (20% wt.), oxine (5% wt.), chloroform (20% vol.) and sodium hydroxide (0.02% wt.). After sufficient time of agitation, the intermetallic compounds remain quantitatively in the solution.

Notes: Zusammenfassung Zur Isolierung der intermetallischen Verbindungen aus den entsprechenden binären Legierungen wird die Probe mehrere Stunden bis Tage unter Rühren mit einer methanolischen Lösung von Benzoesäure (20%), Oxin (5%), Chloroform (20%) und Natriumhydroxid (0,02%) behandelt. Hierbei wird die Matrix aufgelöst und die intermetallischen Verbindungen bleiben zurück.