ALBERT

Beschreibung: Objective. Circular RNAs (circRNAs) have been reported to be widely involved in pathological processes of various cancers. However, little is known about their diagnostic values in early gastric cancer (EGC). This study is aimed at exploring whether circulating circRNAs in plasma could act as biomarkers for EGC diagnosis. Materials and Methods. Mass spectrometry (MS) was performed to identify the proteins that at significantly aberrantly levels in gastric cancer (GC) tissues. The target circRNA was identified by bioinformatics analysis. A receiver operating characteristic (ROC) curve was generated to evaluate the diagnostic utility. Results. MS revealed that the ribosomal protein L6 (RPL6) expression was significantly downregulated only in EGC tissues vs. nontumorous tissues; this was validated by western blotting (n=30, p=0.0094). Bioinformatics analysis predicted that there is a hsa_circ_0006848/hsa_miR-329-5p/RPL6 axis in GC progression. The hsa_circ_0006848 expression was significantly downregulated in EGC tissues (vs. nontumorous tissues, n=30, p=0.0073) and plasma samples from EGC patients (vs. paired healthy volunteers, n=30, p=0.0089). In addition, the hsa_circ_0006848 plasma level in postoperative patients was significantly higher than that of preoperative patients (n=30, p=0.047). Furthermore, the decreased hsa_circ_0006848 expression in plasma was negatively correlated with poor differentiation (p=0.037) and tumor size (p=0.046). The area under the ROC curve (AUC) of hsa_circ_0006848 in plasma was 0.733, suggesting a good diagnostic value. The plasma hsa_circ_0006848 level combined with the carcinoembryonic antigen (CEA), carbohydrate-associated antigen 19-9 (CA19-9), and carbohydrate-associated antigen 72-4 (CA72-4) level increased the AUC to 0.825. Conclusion. Our results indicated that plasma hsa_circ_0006848 may be a novel noninvasive biomarker in EGC diagnosis.

Beschreibung: Background. Insulin receptor substrate 1 (IRS-1) is an important molecule of the insulin signal transduction pathway and has been associated with the occurrence and development of many tumors, including hepatocellular carcinoma (HCC). Our study was designed to determine the expression and significance of IRS-1 in human HCC. Methods. Two hundred and forty specimens were drawn from 140 patients, including 100 HCC tissues and 100 paracancerous (PC) tissues from 100 HCC patients, 20 liver cirrhosis (LC) tissues from 20 LC patients, and 20 chronic hepatitis (CH) tissues from 20 CH patients. Baseline and pathological characteristics were included, and the expression of IRS-1 was examined by immunohistochemical (IHC) staining. Binary logistic regression model calculation was used for multivariate analysis. Results. The total positive rates of IRS-1 expression were 41.0%, 17.0%, 15.0%, and 10.0% in HCC, PC, LC and CH tissues, respectively. IRS-1-positive signals were brown in color and located in the nucleus and cytoplasm. Compared with PC, LC, and CH tissues, a significantly increased expression was observed in human HCC tissues (P

Beschreibung: Benzoxazolones are widely distributed in plants and are of increasing interest for a variety of pharmacological properties, such as detoxification, antibacterial, anti-inflammatory, and analgesic activities and tranquilizers (Michaelidou and Hadjipavlou-Litina, 2005; Doğruer et al., 1998). 4-Hydroxy-2-benzoxazolone (HBOA) is one of the major bioactive compounds in traditional Chinese herb drug Acanthus ilicifolius  (Peng and Long, 2006) which has obvious anti-inflammatory and analgesic activities (Huo et al., 2004; Mani Senthil Kumar et al., 2008; Babu et al., 2001). In this research, we used 2-nitroresorcinol as starting material to prepare HBOA with a novel “one-pot-way.” Derivatives of HBOA TC-2∼TC-4 were obtained via electrophilic reagents and reacted with corresponding primary amines to afford Schiff Base derivatives TC-5∼TC-10 for further study. Anti-inflammatory and analgesic activities of those derivatives were determined by using carrageenan-induced rat paw edema test. The analgesic activities of the derivatives were determined by the hot-plate test.

Beschreibung: Simultaneous identification of donkey-hide gelatin and bovine-hide gelatin in deer-horn glue was established by rapid-resolution liquid chromatography-triple quadrupole mass spectrometry. Water containing 1% NH4HCO3 was used for sample dissolution and trypsin was used for hydrolysis of the gelatins. After separation by a SB-C18 reversed-phase analytical column, collagen marker peptides were detected by mass spectrometry in positive electrospray ionization mode with multiple reaction monitoring. The method was specific, precise and reliable, and suitable for detection of adulterants derived from donkey-hide gelatin and bovine-hide gelatin in deer-horn glue.

Beschreibung: A wet de-NOx technique based on an UV-enhanced NaClO oxidation process was investigated for simulated flue gas of a diesel engine using a bench-scale reaction chamber. The effects of UV irradiation time, initial pH value, and available chlorine concentration of NaClO solution were studied, respectively. The results showed that when the UV irradiation time was 17.5 min and the initial pH value of NaClO solution was 6, NO removal efficiency of UV/NaClO solution was increased by 19.6% compared with that of NaClO solution. Meanwhile, when the available chlorine concentration of NaClO solution decreased from 0.1 wt% to 0.05 wt%, the enhancement in NO removal efficiency of UV/NaClO solution increased from 19.6% to 24%, compared with that of NaClO solution. The reaction pathways of NaClO solution photolysis and NO removal by UV/NaClO process were preliminarily discussed. The results suggested that HOCl might be the most active species that released many UV-induced photooxidants through photolysis reactions, which played an important role in NO removal process.

Beschreibung: The samples of DaGang atmospheric residue (DG-AR), Middle East atmospheric residue (ME-AR), TaHe atmospheric residue (TH-AR), and their thermal reaction samples were chosen for study. All the samples were fractioned into six components separately, including saturates plus light aromatics, heavy aromatics, light resins, middle resins, heavy resins, and asphaltenes. The dielectric permittivity of the solutions of these components was measured, and the dielectric permittivity values of the components can be determined by extrapolation, which increased steadily from saturates plus light aromatics to asphaltenes. Moreover, the Hamaker constants of the components were calculated from their dielectric permittivity values. The Van der Waals attractive potential energy between colloids corresponding to various models could be calculated from the fractional composition and the Hamaker constants of every component. It was assumed that the cores of colloidal particles were formed by asphaltenes and heavy resins mainly; the other fractions acted as dispersion medium. For the three serials of thermal reaction samples, the Van der Waals attraction potential energy between colloids for this kind of model was calculated. For TH-AR thermal reaction samples, the Van der Waals attraction potential energy presented the maximum as thermal reaction is going on, which was near to the end of coke induction period.

Beschreibung: Step-by-step solvent extraction was used to regenerate spent clay by recovering the adsorbed oil in lubricating oil refining clay. Several polar and nonpolar solvents were tested, and petroleum ether (90–120°C) and ethanol (95 v%) were selected as the nonpolar and polar solvents, respectively. The spent clay was first extracted using petroleum ether (90–120°C) to obtain ideal oil and then extracted with a mixed solvent of petroleum ether (90–120°C) and ethanol (95 v%) two or three times to obtain nonideal oil before being extracted with ethanol and water. Finally, the clay was dried at 130°C to obtain regenerated clay. The total oil recovery can be more than 99 wt% of the adsorbed oil. The recovered ideal oil can be used as lubricating base oil. Shorter storage times for spent clay produce better regeneration results. The regenerated clay can be reused to refine the lubricating base oils.

Beschreibung: Taking a slurry reservoir-pipeline-valve system as research object, axial dynamic vibrations of pipe system were induced by coupled hydraulic transient due to rapid closure of valve at the end of pipe. The influences of fluid parameters in multiphase flow, including void fraction, density ratio, and elastic modulus ratio between solid phase and liquid phase, on vibration behaviors of pipe system were analyzed. Results of this study show that wave velocities of pressure and stress can be attenuated evidently when void fraction in multiphase fluid is increased appropriately; meanwhile, the amplitudes of pressure fluctuation and pipe vibration are also weakened obviously. With the increase of density ratio between solid phase and liquid phase, the vibrational intension of pipe system becomes stronger and stronger. In this instance, the increments of vibrational energy mainly concentrate in fluid, which leads the pressure energy of fluid to rise up quickly. When elastic modulus ratio between solid phase and liquid phase increases, the total elasticity of fluid decreases gradually. At the same time, both pressure energy of fluid and vibrational intension of pipe are enhanced but the increments are very slight.

Beschreibung: The heat capacity of lithium pentaborate pentahydrate has been measured using an adiabatic calorimeter at the temperature from 297 to 375 K. No phase transition and thermal anomalies were observed. The molar heat capacity of LiB5O8·5H2O can be expressed as (J·mol−1·K−1) = 396.79376 + 35.87528 + + , where is the temperature in Kelvin, , and . The thermodynamic functions of , , and of LiB5O8·5H2O are obtained via the molar heat capacity at the temperature of 5 K intervals.

Beschreibung: In our research, a straightforward UPLC-MS/MS method, with diazepam as the internal standard (IS), was proposed and acknowledged to determine the concentrations of enasidenib in rat plasma. When preparing the sample, we used acetonitrile for protein precipitation. The gradient elution method was used, and the mobile phase was acetonitrile and 0.1% formic acid. Diazepam was used as the IS. We used the Acquity UPLC BEH C18 column to separate enasidenib and IS. Under the positive ion electrospray ionization (ESI) source conditions, the mass transfer pairs of enasidenib were monitored by multiple reaction monitoring (MRM) to be m/z 474.2 ⟶ 456.1 and m/z 474.2 ⟶ 267.0, and the IS mass transfer pairs were m/z 285.0 ⟶ 154.0. Enasidenib had good linearity (r2 = 0.9985) in the concentration range of 1.0–1000 ng/mL. Besides, the values of intraday and interday precision were 2.25–8.40% and 3.94–5.46%, respectively, and the range of the accuracy values varied from −1.44 to 2.34%. Matrix effect, extraction recovery, and stability were compliant with FDA approval guidelines in terms of bioanalytical method validation. We had established a new method that had been applied to the pharmacokinetic study of enasidenib in rats.