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  • Helianthus annuus  (3)
  • cytokinin  (2)
  • 2020-2021
  • 2000-2004
  • 1995-1999  (5)
  • 1960-1964
  • 1
    Electronic Resource
    Electronic Resource
    Transgenic periwinkle tissues overproducing cytokinins do not accumulate enhanced levels of indole alkaloids (1996)
    Springer
    Plant cell, tissue and organ culture 45 (1996), S. 223-230 
    Details
    ISSN: 1573-5044
    Keywords: Agrobacterium tumefaciens ; Catharanthus roseus ; cytokinin ; isopentenyl-transferase ; stress response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cytokinins play a critical role in several aspects of plant growth, metabolism and development. We previously reported that adding cytokinins to the culture medium of a suspension-cultured cell line of periwinkle increased the accumulation of indole alkaloids, and our aim was to compare the effect of exogenously-applied cytokinins with that of elevated levels of endogenous cytokinins on indole alkaloid production. We used an Agrobacterium tumefaciens strain yielding a plasmid with the isopentenyl transferase gene under control of its own promoter. Co-culture of suspension cells with the bacteria caused a severe stress response leading to cell necrosis. Therefore, we failed to transform this material but we succeeded in transforming periwinkle cotyledons. We verified that callus cultures generated from the isopentenyl transferase-transgenic cotyledons accumulated high cytokinin concentrations. Treating normal callus cultures (generated from untransformed cotyledons) with cytokinins enhanced their alkaloid production. By contrast, the enhanced concentration of endogenous cytokinins in transgenic calli did not increase indole alkaloid production, and thus did not mimic the effect of exogenously-applied cytokinins. Hypothesis to explain this discrepancy are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043634
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular characterization of a cytokinin-inducible periwinkle protein showing sequence homology with pathogenesis-related proteins and the Bet v 1 allergen family (1998)
    Springer
    Plant molecular biology 36 (1998), S. 791-798 
    Details
    ISSN: 1573-5028
    Keywords: Catharanthus roseus ; cell biochemical differentiation ; cytokinin ; intracellular pathogenesis-related protein ; pollen allergen ; RT- and RACE-PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cytokinin treatment of periwinkle callus cultures increased the accumulation of a protein, designated T1, in two-dimensional separated protein extracts. The first 30 NH2-terminal amino acids were determined by Edman degradation and showed significant sequence homology with intracellular pathogenesis-related (IPR) plant proteins and the Bet v 1 allergen family. The deduced amino acid sequence of cDNAs coding for T1, isolated by RT-PCR and 5′ RACE-PCR, exhibited an average sequence identity of 40% with both IPR and Bet v 1-related allergens. T1 and all related proteins contained a p-loop motif typically found in nucleotide-binding proteins as the most conserved sequence feature. Northern blot analysis showed that cytokinin treatment of periwinkle callus induced T1 transcripts, whereas addition of 2,4-dichlorophenoxyacetic acid inhibited this accumulation. Hybridization of genomic periwinkle DNA with the T1 cDNA suggested that the protein is encoded by a single-copy gene. Immunoblot studies with a panel of Bet v 1-specific antibodies and sera from Bet v 1 allergic individuals identified T1 as a protein that is immunologically distinct from the Bet v 1 allergen family and has no allergenic properties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005951208815
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  • 3
    Electronic Resource
    Electronic Resource
    Colocation of downy mildew (Plasmopara halstedii) resistance genes in sunflower (Helianthus annuus L.) (1996)
    Springer
    Euphytica 91 (1996), S. 225-228 
    Details
    ISSN: 1573-5060
    Keywords: bulked segregant analysis ; Helianthus annuus ; linkage ; Plasmopara halstedii ; resistance genes ; RFLP ; sunflower ; downy mildew
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The Pl6 locus in the inbred sunflower (Helianthus annuus L.) line HA335 giving resistance to French races of downy mildew (Plasmopara halstedii (Farl.) Berl. & de Toni. was localized by molecular techniques. A bulked segregant analysis was made on the F2 progeny from a cross between this line and H52, a downy mildew susceptible line. The resistance gene in HA335 was found to have the same linked RFLP marker loci as those determined for Pl1 (resistance to race 1 in the line RHA266) on linkage group 1 of the consensus RFLP map of the cultivated sunflower. Pl1 and Pl6 thus appear either to be allelic or closely linked. The implications for sunflower breeding are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021074
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  • 4
    Electronic Resource
    Electronic Resource
    Accumulation of Defense Related Transcripts in Sunflower Hypocotyls (Helianthus annuus L.) Infected with Plasmopara halstedii (1999)
    Springer
    European journal of plant pathology 105 (1999), S. 333-340 
    Details
    ISSN: 1573-8469
    Keywords: chitinase ; defense gene ; Helianthus annuus ; PAL ; Plasmopara halstedii ; ubiquitin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A cDNA clone encoding a sunflower chitinase was obtained using degenerated primers in PCR amplifications and RACE procedures. This clone, a phenylalanine ammonia-lyase (PAL) clone and ubiquitin clone were used to analyse the resistance of sunflower (Helianthus annuus) to downy mildew. The differential regulation of amounts of PAL (involved in the general pathway of phenylpropanoid synthesis), chitinase (a pathogenesis-related protein) and ubiquitin (involved in proteolytic pathways) mRNA was studied in hypocotyls during the early stages after an aerial infection of sunflower inbred line RHA274 with zoospores from either race 1 (incompatible, host resistant) or race B (compatible, host susceptible) of Plasmopara halstedii. Northern analyses showed that transcript levels of PAL, chitinase and ubiquitin were rapidly and strongly increased after infection in incompatible interactions but not in the compatible ones, suggesting that regulation of these mRNAs is an important component of the resistance mechanisms in sunflower.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008770008117
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  • 5
    Electronic Resource
    Electronic Resource
    Microscopical studies of the effect of metalaxyl on the interaction between sunflower,Helianthus annuus L. and downy mildew,Plasmopara halstedii (1995)
    Springer
    European journal of plant pathology 101 (1995), S. 399-404 
    Details
    ISSN: 1573-8469
    Keywords: Helianthus annuus ; hypersensitivity ; metalaxyl ; Plasmopara halstedii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The mode of action of the fungicide metalaxyl againstPlasmopara halstedii, the causal agent of sunflower downy mildew, was studied following its application before, during and after artificial contamination of seedlings. The development of the fungus within the treated seedlings was examined microscopically and compared to that occuring in untreated genetically susceptible or resistant genotypes. Hypersensitive-like reactions and necrotic zones leading to the inhibition of fungus growth within the hypocotyl were observed for the three modes of application. This suggests that sunflower defence mechanisms activated by genetical resistance are also involved in the control of downy mildew by metalaxyl.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01874853
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