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  • Analytical Chemistry and Spectroscopy  (2)
  • Catharanthus roseus  (2)
  • Inorganic and Physical Chemistry  (2)
  • 2020-2023
  • 2020-2021
  • 2000-2004
  • 1995-1999  (6)
  • 1965-1969
  • 1960-1964
Collection
Keywords
Publisher
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Transgenic periwinkle tissues overproducing cytokinins do not accumulate enhanced levels of indole alkaloids (1996)
    Springer
    Plant cell, tissue and organ culture 45 (1996), S. 223-230 
    Details
    ISSN: 1573-5044
    Keywords: Agrobacterium tumefaciens ; Catharanthus roseus ; cytokinin ; isopentenyl-transferase ; stress response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cytokinins play a critical role in several aspects of plant growth, metabolism and development. We previously reported that adding cytokinins to the culture medium of a suspension-cultured cell line of periwinkle increased the accumulation of indole alkaloids, and our aim was to compare the effect of exogenously-applied cytokinins with that of elevated levels of endogenous cytokinins on indole alkaloid production. We used an Agrobacterium tumefaciens strain yielding a plasmid with the isopentenyl transferase gene under control of its own promoter. Co-culture of suspension cells with the bacteria caused a severe stress response leading to cell necrosis. Therefore, we failed to transform this material but we succeeded in transforming periwinkle cotyledons. We verified that callus cultures generated from the isopentenyl transferase-transgenic cotyledons accumulated high cytokinin concentrations. Treating normal callus cultures (generated from untransformed cotyledons) with cytokinins enhanced their alkaloid production. By contrast, the enhanced concentration of endogenous cytokinins in transgenic calli did not increase indole alkaloid production, and thus did not mimic the effect of exogenously-applied cytokinins. Hypothesis to explain this discrepancy are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043634
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular characterization of a cytokinin-inducible periwinkle protein showing sequence homology with pathogenesis-related proteins and the Bet v 1 allergen family (1998)
    Springer
    Plant molecular biology 36 (1998), S. 791-798 
    Details
    ISSN: 1573-5028
    Keywords: Catharanthus roseus ; cell biochemical differentiation ; cytokinin ; intracellular pathogenesis-related protein ; pollen allergen ; RT- and RACE-PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cytokinin treatment of periwinkle callus cultures increased the accumulation of a protein, designated T1, in two-dimensional separated protein extracts. The first 30 NH2-terminal amino acids were determined by Edman degradation and showed significant sequence homology with intracellular pathogenesis-related (IPR) plant proteins and the Bet v 1 allergen family. The deduced amino acid sequence of cDNAs coding for T1, isolated by RT-PCR and 5′ RACE-PCR, exhibited an average sequence identity of 40% with both IPR and Bet v 1-related allergens. T1 and all related proteins contained a p-loop motif typically found in nucleotide-binding proteins as the most conserved sequence feature. Northern blot analysis showed that cytokinin treatment of periwinkle callus induced T1 transcripts, whereas addition of 2,4-dichlorophenoxyacetic acid inhibited this accumulation. Hybridization of genomic periwinkle DNA with the T1 cDNA suggested that the protein is encoded by a single-copy gene. Immunoblot studies with a panel of Bet v 1-specific antibodies and sera from Bet v 1 allergic individuals identified T1 as a protein that is immunologically distinct from the Bet v 1 allergen family and has no allergenic properties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005951208815
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  • 3
    Electronic Resource
    Electronic Resource
    The Use of Cationic Polymer for the Separation of Inorganic Anions by Capillary Electrophoresis (1998)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 21 (1998), S. 505-508 
    Details
    ISSN: 0935-6304
    Keywords: capillary electrophoresis ; inorganic ions ; polyethyleneimine ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---A capillary zone electrophoresis (CZE) method for the simultaneous assay of bromide, iodide, nitrite, nitrate, and thiocyanate using direct UV detection is reported. The method is based on the separation of anions in a capillary coated with a cationic polymer, polyethyleneimine (PEI). The minimum detection limits, reproducibility of peak areas, and migration times were determined at the optimal condition. The method was applied to the analysis of tap water and human urine. The changes in separation selectivity of the anions resulting from addition of the cationic polymer to the buffer were investigated.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Resolution effects of organic additives on the MEKC enantiomeric separations of tri aza aromatic ligand compounds of iron(II) (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Microcolumn Separations 7 (1995), S. 199-206 
    Details
    ISSN: 1040-7685
    Keywords: micellar electrokinetic chromatography ; capillary electrophoresis ; bile salts ; metal-ligands ; chiral separations ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We have utilized micellar electrokinetic chromatography with sodium deoxycholate micelles to separate the enantiomers of bis(8-((pyridine-2-methylene)amino)quinoline)iron(II) hexafluorophosphate, Fe(PMAQ)2(PF6)2, bis(8-((pyridine-2-methylene)amino)lepidine iron(II) hexafluorophosphate, Fe(PMAL)2(PF6)2, and bis(1-(2-pyridinyl)ethylidine)-8-aminoquinoline iron(II) hexafluorophosphate, Fe(PEAQ)2(PF6)2. The influence of 13 water miscible organic additives on the resolution of each pair of enantiomers was investigated. Significant changes in resolution are seen depending upon the particular organic additive used in the separation buffer. In addition, the effects of acetone concentration on the resolution of these enantiomers in bile salt solution was investigated. Resolution for all three pairs of enantiomers is maximized in a buffer solution containing 15% acetone. © 1995 John Wiley & Sons, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mcs.1220070303
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  • 5
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    Effects of Gravity on Sheared and Nonsheared Turbulent Nonpremixed Flames (1997)
    In:  CASI
    Details
    Publication Date: 2019-06-28
    Description: The present numerical study is concerned with the fundamental physics of the multi-way interaction between turbulence, chemical reaction and buoyancy in a nonpremixed flame. The method of direct numerical simulation (DNS) is used to solve the instantaneous three-dimensional governing equations (continuity, Navier-Stokes, species mass fractions and energy) under the zero-Mach number assumption. Because of the present supercomputer limitations, we consider two simple flow geometries, namely an initially uniform flow without shear (equivalent to grid-generated turbulence) and an initially uniform shear flow. In each flow, the fuel and oxidant initially exist as two separate streams. As the reactants mix, chemical reaction takes place and exothermic energy is released causing variations in density. In the presence of a gravity field, the spatial and temporal distributions of the induced buoyancy forces depend on the local density gradients and the direction of the gravitational acceleration.
    Keywords: Inorganic and Physical Chemistry
    Type: Fourth International Microgravity Combustion Workshop; 173-178; NASA-CP-10194
    Format: text
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    NASA TECHNICAL REPORTS
  • 6
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    Method for Trace Oxygen Detection (1997)
    In:  CASI
    Details
    Publication Date: 2019-06-28
    Description: Trace levels of molecular oxygen are measured by introducing a gas containing the molecular oxygen into a target zone, and impacting the molecular oxygen in the target zone with electrons at the O(-) resonant energy level for dissociative electron attachment to produce O(-) ions. Preferably, the electrons have an energy of about 4 to about 10 eV. The amount of O(-) ions produced is measured, and is correlated with the molecular oxygen content in the target zone. The technique is effective for measuring levels of oxygen below 50 ppb. and even less than 1 ppb. The amount of O(-) can be measured in a quadrupole mass analyzer. Best results are obtained when the electrons have an energy of about 6 to about 8 eV. and preferably about 6.8 eV. The method can be used for other species by selecting the appropriate electron energy level.
    Keywords: Inorganic and Physical Chemistry
    Format: application/pdf
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    NASA TECHNICAL REPORTS
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