ALBERT

Description: Here, we report the results of a multiple stressor experiment combining impacts of ocean acidification and Polymetallic sulfide (PMS) deposits produced by deep-sea mining on larval survival of the deep-sea coral Desmophyllum pertusum (syn. Lophelia pertusa). Adult colonies of D. pertusum were collected in December and January 2022 at Tisler reef, at depths between 100-120 m. Adults were maintained in aquaria and after spawning, embryos were collected and left to develop to 7-day larvae. On day 7, larvae were exposed to four experimental treatments and their combinations: two pCO2 treatments recreating present conditions (400 ppm), and conditions projected to the end of the century (RCP8.5, 1000 ppm), as well as two mining treatments corresponding to PMS particles (concentration 5 mg/L), and their leachates. Survival was monitored every 24h.

Description: In this dataset, we report the results of an aquaria experiment aiming at determining the impacts of Polymetallic sulfide (PMS) plumes of varying concentrations created by deep-sea mining on larval survival of the deep-sea coral Desmophyllum pertusum. Colonies of D. pertusum were collected in December 2022 and January 2023 at Tisler reef, at depths between 100-120 m. Adults were maintained in aquaria and after spawning, embryos were collected and left to develop to 7-day larvae. On day 7, larvae were exposed to four experimental treatments, recreating the potential effects of a PMS mining plume: two treatments contained PMS particles at concentrations of 2.5 mg/L and 5 mg/L respectively, and another two contained only the leachates of PMS particle solutions corresponding to the aforementioned PMS particle concentrations. Temperature was maintained at 8-8.5 °C and pHT at 8.01. Survival was checked by counting surviving larvae after 24h of exposure.

Description: This data publication and the related three other datasets contains results from 4 separate experiments aiming to assess the effect of temperature on embryonic and larval development as well as larval swimming in Lophelia pertusa (syn. Desmophyllum pertusum). Parental colonies for subsequent spawning in the laboratory were collected 2019-2020 at the Tisler reef (Lat 58.99, Lon 10.97) 1-3 months before the spawning season for L. pertusa in the Skagerrak (February). Males and females were placed together in laboratory tanks at Tjärnö Marine Laboratory, University of Gothenburg and maintained in flow through of seawater with a salinity around 33 psu and a temperature of 8°C. Corals were fed 2-3 times a week with frozen zooplankton. During the spawning season corals were continuosly observed and when both sexes spawned simultaneously gamets were collected. Gametes were either directly treated with various temperatures or embryo and larvae were maintained at 8°C for a period of time before temperature treatment began. For all experiments, more than one spawn batch was used. For temperature treatments, larvae were moved to 2-3 replicate flasks and placed in thermo regulated cabinets and rooms. This particular dataset shows when the first embryo in any replicate flask in each temperature treatment developed any ciliae.

Description: This data publication and the related three other datasets contains results from 4 separate experiments aiming to assess the effect of temperature on embryonic and larval development as well as larval swimming in Lophelia pertusa (syn. Desmophyllum pertusum). Parental colonies for subsequent spawning in the laboratory were collected 2019-2020 at the Tisler reef (Lat 58.99, Lon 10.97) 1-3 months before the spawning season for L. pertusa in the Skagerrak (February). Males and females were placed together in laboratory tanks at Tjärnö Marine Laboratory, University of Gothenburg and maintained in flow through of seawater with a salinity around 33 psu and a temperature of 8°C. Corals were fed 2-3 times a week with frozen zooplankton. During the spawning season corals were continuosly observed and when both sexes spawned simultaneously gamets were collected. Gametes were either directly treated with various temperatures or embryo and larvae were maintained at 8°C for a period of time before temperature treatment began. For all experiments, more than one spawn batch was used. For temperature treatments, larvae were moved to 2-3 replicate flasks and placed in thermo regulated cabinets and rooms. In this particular dataset, the embryos were moved from 8°C to treatment temperature after 3-4 days (when embryo start swimming) and were then regularly checked to assess when the first larva in any replicate flask in each temperature treatment had developed any cnidocysts (sign of settlement competency).

Description: This data publication and the related three other datasets contains results from 4 separate experiments aiming to assess the effect of temperature on embryonic and larval development as well as larval swimming in Lophelia pertusa (syn. Desmophyllum pertusum). Parental colonies for subsequent spawning in the laboratory were collected 2019-2020 at the Tisler reef (Lat 58.99, Lon 10.97) 1-3 months before the spawning season for L. pertusa in the Skagerrak (February). Males and females were placed together in laboratory tanks at Tjärnö Marine Laboratory, University of Gothenburg and maintained in flow through of seawater with a salinity around 33 psu and a temperature of 8°C. Corals were fed 2-3 times a week with frozen zooplankton. During the spawning season corals were continuosly observed and when both sexes spawned simultaneously gamets were collected. Gametes were either directly treated with various temperatures or embryo and larvae were maintained at 8°C for a period of time before temperature treatment began. For all experiments, more than one spawn batch was used. For temperature treatments, larvae were moved to 2-3 replicate flasks and placed in thermo regulated cabinets and rooms. In this particular dataset, the effect of temperature on upwards directed swimming speed was assessed. During the trials in 2019, the larvae were moved directly from 8°C to the recording of swimming in treatment temperature, while in 2021 larvae were moved to the treatment temperature one day before swimming was recorded.

Description: This data publication and the related three other datasets contains results from 4 separate experiments aiming to assess the effect of temperature on embryonic and larval development as well as larval swimming in Lophelia pertusa (syn. Desmophyllum pertusum). Parental colonies for subsequent spawning in the laboratory were collected 2019-2020 at the Tisler reef (Lat 58.99, Lon 10.97) 1-3 months before the spawning season for L. pertusa in the Skagerrak (February). Males and females were placed together in laboratory tanks at Tjärnö Marine Laboratory, University of Gothenburg and maintained in flow through of seawater with a salinity around 33 psu and a temperature of 8°C. Corals were fed 2-3 times a week with frozen zooplankton. During the spawning season corals were continuosly observed and when both sexes spawned simultaneously gamets were collected. Gametes were either directly treated with various temperatures or embryo and larvae were maintained at 8°C for a period of time before temperature treatment began. For all experiments, more than one spawn batch was used. For temperature treatments, lavae were moved to 2-3 replicate flasks and placed in thermo regulated cabinets and rooms. This particular dataset shows the proportion of embryos found in each developmental stage depending on time since spawning and temperature treatment.

Description: This data publication together with two related publications contains data from a number of experiments aiming to assess the effect of suspended sediment exposure on fertilization, embryonic and larval development as well as larval swimming in Lophelia pertusa (syn. Desmophyllum pertusum). Parental colonies for subsequent spawning in the laboratory were collected in 2021 and 2022 at the Tisler reef (Lat 58.99, Lon 10.97) 1-2 months before the spawning season of L. pertusa in the Skagerrak (February). Males and females were placed together in laboratory tanks at Tjärnö Marine Laboratory, University of Gothenburg and maintained in flow-through of seawater with a salinity around 33 psu and a temperature of 8 °C. Corals were fed 2–3 times a week with frozen zooplankton. About a week before the start of experiments, benthic sediment was collected from around 130 m water depth in bottom trawled seafloor areas of the Koster Fjord, some 10 km south of the Tisler reef, and was sieved to 63 µm before storage at 2 °C. During the spawning season corals were continuously observed and when both sexes spawned simultaneously gametes were collected. Gamete mixtures were either close to directly treated with sediments or embryo and larvae were maintained at 8 °C for a period of time before the sediment treatment began. For sediment treatments, larvae were moved to 3-5 replicate flasks (75 ml culture flasks) with 0, 2.5, 5.0 and 25 mg dry weight of sediment per liter seawater and placed on a plankton wheel in a thermo-regulated room at 8 °C. Exposure times of 1, 2 and 3 days were used. In this particular dataset, the effect of direct sediment exposure on fertilization rate is assessed.

Description: This data publication together with two related publications contains data from a number of experiments aiming to assess the effect of suspended sediment exposure on fertilization, embryonic and larval development as well as larval swimming in Lophelia pertusa (syn. Desmophyllum pertusum). Parental colonies for subsequent spawning in the laboratory were collected in 2021 and 2022 at the Tisler reef (Lat 58.99, Lon 10.97) 1-2 months before the spawning season of L. pertusa in the Skagerrak (February). Males and females were placed together in laboratory tanks at Tjärnö Marine Laboratory, University of Gothenburg and maintained in flow-through of seawater with a salinity around 33 psu and a temperature of 8 °C. Corals were fed 2–3 times a week with frozen zooplankton. About a week before the start of experiments, benthic sediment was collected from around 130 m water depth in bottom trawled seafloor areas of the Koster Fjord, some 10 km south of the Tisler reef, and was sieved to 63 µm before storage at 2 °C. During the spawning season corals were continuously observed and when both sexes spawned simultaneously gametes were collected. Gamete mixtures were either close to directly treated with sediments or embryo and larvae were maintained at 8 °C for a period of time before the sediment treatment began. For sediment treatments, larvae were moved to 3-5 replicate flasks (75 ml culture flasks) with 0, 2.5, 5.0 and 25 mg dry weight of sediment per liter seawater and placed on a plankton wheel in a thermo-regulated room at 8 °C. Exposure times of 1, 2 and 3 days were used. In this particular dataset, embryos of different stages, maintained at 8 °C for a period of time before, were exposed to sediment and embryo development and survival was monitored.

Description: This data publication together with two related publications contains data from a number of experiments aiming to assess the effect of suspended sediment exposure on fertilization, embryonic and larval development as well as larval swimming in Lophelia pertusa (syn. Desmophyllum pertusum). Parental colonies for subsequent spawning in the laboratory were collected in 2021 and 2022 at the Tisler reef (Lat 58.99, Lon 10.97) 1-2 months before the spawning season of L. pertusa in the Skagerrak (February). Males and females were placed together in laboratory tanks at Tjärnö Marine Laboratory, University of Gothenburg and maintained in flow-through of seawater with a salinity around 33 psu and a temperature of 8 °C. Corals were fed 2–3 times a week with frozen zooplankton. About a week before the start of experiments, benthic sediment was collected from around 130 m water depth in bottom trawled seafloor areas of the Koster Fjord, some 10 km south of the Tisler reef, and was sieved to 63 µm before storage at 2 °C. During the spawning season corals were continuously observed and when both sexes spawned simultaneously gametes were collected. Gamete mixtures were either close to directly treated with sediments or embryo and larvae were maintained at 8 °C for a period of time before the sediment treatment began. For sediment treatments, larvae were moved to 3-5 replicate flasks (75 ml culture flasks) with 0, 2.5, 5.0 and 25 mg dry weight of sediment per liter seawater and placed on a plankton wheel in a thermo-regulated room at 8 °C. Exposure times of 1, 2 and 3 days were used. In this particular dataset, larvae of various ages were exposed to sediments and their survival, plus in a few cases swimming speed, after treatment was assessed.

Description: Raw multibeam and sound velocity data collected around Cabo Verde on the iMirabilis2 Cruise, 04/08/2021-26/08/2021, as part of the iAtlantic Project. The data were collected using an Atlas Hydrosweep DS-3 Multibeam Echosounder, using Atlas Naviscan and PDS2000 from Teledyne. The sound velocity profiles (SVP) were collected using CTD casts and XBTs. The dataset spans ~200m - 4500m water depth.