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  • 1
    Publication Date: 2022-12-06
    Type: Conference or Workshop Item , NonPeerReviewed
    Format: text
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  • 2
    Publication Date: 2022-10-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Grearson, A. G., Dugan, A., Sakmar, T., Sivitilli, D. M., Gire, D. H., Caldwell, R. L., Niell, C. M., Doelen, G., Wang, Z. Y., & Grasse, B. The lesser Pacific Striped Octopus, Octopus chierchiae: an emerging laboratory model. Frontiers in Marine Science, 8, (2021): 753483, https://doi.org/10.3389/fmars.2021.753483.
    Description: Cephalopods have the potential to become useful experimental models in various fields of science, particularly in neuroscience, physiology, and behavior. Their complex nervous systems, intricate color- and texture-changing body patterns, and problem-solving abilities have attracted the attention of the biological research community, while the high growth rates and short life cycles of some species render them suitable for laboratory culture. Octopus chierchiae is a small octopus native to the central Pacific coast of North America whose predictable reproduction, short time to maturity, small adult size, and ability to lay multiple egg clutches (iteroparity) make this species ideally suited to laboratory culture. Here we describe novel methods for multigenerational culture of O. chierchiae, with emphasis on enclosure designs, feeding regimes, and breeding management. O. chierchiae bred in the laboratory grow from a 3.5 mm mantle length at hatching to an adult mantle length of approximately 20–30 mm in 250–300 days, with 15 and 14% survivorship to over 400 days of age in first and second generations, respectively. O. chierchiae sexually matures at around 6 months of age and, unlike most octopus species, can lay multiple clutches of large, direct-developing eggs every ∼30–90 days. Based on these results, we propose that O. chierchiae possesses both the practical and biological features needed for a model octopus that can be cultured repeatedly to address a wide range of biological questions.
    Description: The cephalopod program at the Marine Biological Laboratory (MBL) was supported by NSF 1827509 and NSF 1723141 grants. CN received funding from HFSP RGP0042. DG and DS received funding and research support from the University of Washington Friday Harbor Laboratories. ZYW was supported by funds from the Whitman Center at the MBL.
    Keywords: Iteroparity ; Cephalopod ; Model organism ; Aquaculture ; Reproduction – mollusk ; Developmental biology ; Neurobiology
    Repository Name: Woods Hole Open Access Server
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  • 3
    Publication Date: 2022-10-26
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Reyes-Macaya, D., Hoogakker, B., Martinez-Mendez, G., Llanillo, P. J., Grasse, P., Mohtadi, M., Mix, A., Leng, M. J., Struck, U., McCorkle, D. C., Troncoso, M., Gayo, E. M., Lange, C. B., Farias, L., Carhuapoma, W., Graco, M., Cornejo-D’Ottone, M., De Pol Holz, R., Fernandez, C., Narvaez, D., Vargas, C. A., García-Araya, F., Hebbeln, D. Isotopic characterization of water masses in the Southeast Pacific Region: paleoceanographic implications. Journal of Geophysical Research: Oceans, 127(1), (2022): e2021JC017525, https://doi.org/10.1029/2021JC017525.
    Description: In this study, we used stable isotopes of oxygen (δ18O), deuterium (δD), and dissolved inorganic carbon (δ13CDIC) in combination with temperature, salinity, oxygen, and nutrient concentrations to characterize the coastal (71°–78°W) and an oceanic (82°–98°W) water masses (SAAW—Subantarctic Surface Water; STW—Subtropical Water; ESSW—Equatorial Subsurface water; AAIW—Antarctic Intermediate Water; PDW—Pacific Deep Water) of the Southeast Pacific (SEP). The results show that δ18O and δD can be used to differentiate between SAAW-STW, SAAW-ESSW, and ESSW-AAIW. δ13CDIC signatures can be used to differentiate between STW-ESSW (oceanic section), SAAW-ESSW, ESSW-AAIW, and AAIW-PDW. Compared with the oceanic section, our new coastal section highlights differences in both the chemistry and geometry of water masses above 1,000 m. Previous paleoceanographic studies using marine sediments from the SEP continental margin used the present-day hydrological oceanic transect to compare against, as the coastal section was not sufficiently characterized. We suggest that our new results of the coastal section should be used for past characterizations of the SEP water masses that are usually based on continental margin sediment samples.
    Description: R/V Sonne cruises (SO102, SO211 ad SO245) were financed by the German Federal Ministry of Education and Research projects #03G0102A, #03G0211A and #03G0245A. SO261 cruise was funded by the HADES-ERC Advanced Grant (“Benthic diagenesis and microbiology of hadal trenches” Grant agreement No. 669947) awarded to R. N. Glud (SDU, Denmark). SO245 cruise recived contributions from the Max Planck Society (Germany), the German State of Lower Saxony, the National Environmental Research Council of Great Britain and the Science Foundation of Ireland. R/V Meteor cruise M93 was financed by the Sonderforschungsbereich 754 “Climate-Biogeochemistry Interactions in the Tropical Ocean” (www.sfb754.de), which is supported by the Deutsche Forschungsgemeinschaft. “Expedición TAITAO” was financed by the grant “Concurso Nacional de Asignación de Tiempo de Buque ASG-61 Cabo de Hornos” AUB180003, FONDECyT grants 11161091 (DN), 1180954 (CF), and the COPAS Sur-Austral Center (CONICYT PIA APOYO CCTE AFB170006). Sampling at Time-Series station 18 off Concepción during 2015 was funded by several FONDECYT/ANID grants from researchers at the Department of Oceanography and Research Line 5 of COPAS Sur-Austral (UdeC). ANID—Chile National Competition for ship time (AUB 150006/12806) financed the expedition LowpHOX organized by the Millennium Institute of Oceanography (IMO). The expedition Crio1218 was financed by the PPR 137 titled “Proyecto de Estudio Integrado del Afloramiento Costero Frente a Perú" and sponsored by IMARPE-Perú. Additional funding was provided by the ANID—Millennium Science Initiative Program—NCN19_153 (Millennium Nucleus UPWELL), ANID/FONDAP (CR)2 15110009 (LF and EMG), FONDECYT Grant 1210171 (CAV), ANID/FONDAP IDEAL 15150003 (CBL), and the Millennium Institute of Oceanography (IMO, ICN12_019). Dharma A. Reyes-Macaya was supported by Becas Chile (17342817-0), DAAD (57144001) and FARGO project (FAte of ocean oxygenation in a waRminG wOrld, UKRI).
    Keywords: Oxygen and deuterium stable isotopes in seawater ; Carbon stable isotopes in dissolved inorganic carbon ; Southeast Pacific ; Water mass distribution ; Paleoceanography proxies
    Repository Name: Woods Hole Open Access Server
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