ALBERT

Description: Background Liberibacter crescens is the closest cultured relative of four important uncultured crop pathogens. Candidatus. L. asiaticus, L. americanus, L. africanus cause citrus greening disease, while Ca. L. solanacearum causes potato Zebra chip disease. None of the pathogens grows in axenic culture. L. crescens grows in three media: a BM-7, a serum-free Hi® Grace’s Insect Medium (Hi-GI), and a chemically-defined medium called M15. To date, no optimal growth parameters of the model species L. crescens have been reported. Studying the main growth parameters of L. crescens in axenic culture will give us insights into the lifestyle of the Ca. Liberibacter pathogens. Results The evaluation of the growth parameters—pH, aeration, temperature, and buffering capacity—reflects the optimal living conditions of L. crescens. These variables revealed that L. crescens is an aerobic, neutrophilic bacterium, that grows optimally in broth in a pH range of 5.8 to 6.8, in a fully oxygenated environment (250 rpm), at 28 °C, and with monosodium phosphate (10 mM or 11.69 mM) as the preferred buffer for growth. The increase of pH in the external media likely results from the deamination activity within the cell, with the concomitant over-production of ammonium in the external medium. Conclusion L. crescens and the Ca. Liberibacter pathogens are metabolically similar and grow in similar environments—the phloem and the gut of their insect vectors. The evaluation of the growth parameters of L. crescens reveals the lifestyle of Liberibacter, elucidating ammonium and phosphate as essential molecules for colonization within the hosts. Ammonium is the main driver of pH modulation by active deamination of amino acids in the L. crescens amino acid rich media. In plants, excess ammonium induces ionic imbalances, oxidative stress, and pH disturbances across cell membranes, causing stunted root and shoot growth and chlorosis—the common symptoms of HLB-disease. Phosphate, which is also present in Ca. L. asiaticus hosts, is the preferred buffer for the growth of L. crescens. The interplay between ammonium, sucrose, potassium (K+), phosphate, nitrate (NO3−), light and other photosynthates might lead to develop better strategies for disease management.

Description: We used environmental niche modelling along with the best available species occurrence data and environmental parameters to model habitat suitability for key cold-water coral and commercially important deep-sea fish species under present-day (1951-2000) environmental conditions and to forecast changes under severe, high emissions future (2081-2100) climate projections (RCP8.5 scenario) for the North Atlantic Ocean (from 18°N to 76°N and 36°E to 98°W). The VME indicator taxa included Lophelia pertusa , Madrepora oculata, Desmophyllum dianthus, Acanela arbuscula, Acanthogorgia armata, and Paragorgia arborea. The six deep-sea fish species selected were: Coryphaenoides rupestris, Gadus morhua, blackbelly Helicolenus dactylopterus, Hippoglossoides platessoides, Reinhardtius hippoglossoides, and Sebastes mentella. We used an ensemble modelling approach employing three widely-used modelling methods: the Maxent maximum entropy model, Generalized Additive Models, and Random Forest. This dataset contains: 1) Predicted habitat suitability index under present-day (1951-2000) and future (2081-2100; RCP8.5) environmental conditions for twelve deep-sea species in the North Atlantic Ocean, using an ensemble modelling approach.  2) Climate-induced changes in the suitable habitat of twelve deep-sea species in the North Atlantic Ocean, as determined by binary maps built with an ensemble modelling approach and the 10-percentile training presence logistic (10th percentile) threshold. 3) Forecasted present-day suitable habitat loss (value=-1), gain (value=1), and acting as climate refugia (value=2) areas under future (2081-2100; RCP8.5) environmental conditions for twelve deep-sea species in the North Atlantic Ocean. Areas were identified from binary maps built with an ensemble modelling approach and two thresholds: 10-percentile training presence logistic threshold (10th percentile) and maximum sensitivity and specificity (MSS). Refugia areas are those areas predicted as suitable both under present-day and future conditions. All predictions were projected with the Albers equal-area conical projection centred in the middle of the study area. The grid cell resolution is of 3x3 km.

Description: Between 14.03.2006 and 25.04.2006, bathymetric data based on the KONGSBERG EM120 system (MBES) was acquired in the Gulf of Mexico during the R/V METEOR cruise M67/2. The main research goal was the investigation of asphalt volcanoes in the Campeche Bay and related sedimentary structures. The leg was split into two parts. During the first sub-leg 2a geophysical and especially hydroacoustic methods were used to explore the distribution of these asphalt volcanoes and to map knolls as well as other structures like mass wasting and asphalt flows. Using reflection seismic, sedimentary structures related to the volcanoes were also investigated. Further mapping but also sampling of vent fluids and asphalt was the research interest of sub-leg 2b. Therefore the Remotely Operating Vehicle (ROV) QUEST (Marum) as well as a TV-MUC were used. Bathymetry mapping was done using the EM120 for deeper and the EM710 for shallower regions. CI Citation: Paul Wintersteller (seafloor-imaging@marum.de) as responsible party for bathymetry raw data ingest and approval. Description of the data source: During the M67/2 cruise, the hull-mounted multibeam echosounder (MBES) KONGSBERG EM120 was utilized to perform bathymetric mapping. It allows to conduct surveys in water depths of up to 11,000 m. Two transducer arrays transmit frequency coded acoustic signals (11.25 to 12.6 kHz). While the emission beam has a dimension of 150° across and 2° along track, the reception is obtained from 191 overlapping beams with widths of 2° across and 20° along track. The beam footprint has a dimension of 2° by 2°. For further information on the system, consult: https://www.km.kongsberg.com/ Due to the water depth of the research area the EM120 was permanently used. Only for shallower regions the EM710 was used too. To convert the recorded travel times into water depth, several sound velocity profiles were obtained with the shipboard CTD, providing a correction for ray bending for each beam. Depth is estimated from each beam by using the two-way travel time and the known beam angle known, and taking into account the ray bending due to refraction in the water column by sound speed variations. Responsible person during this cruise / PI: Volkhard Spieß (vspiess uni-bremen.de), Gerhard Bohrmann (gbohrmann@marum.de) Chief Scientist: : Volkhard Spieß (vspiess uni-bremen.de), Gerhard Bohrmann (gbohrmann@marum.de) CR: https://www.tib.eu/de/suchen/id/awi%3Adoi~10.2312%252Fcr_m67/ CSR: https://www2.bsh.de/aktdat/dod/fahrtergebnis/2006/20060077.htm

Description: Between 14.03.2006 and 25.04.2006, bathymetric data based on the KONGSBERG EM710 system (MBES) was acquired in the Gulf of Mexico during the R/V METEOR cruise M67/2. The main research goal was the investigation of asphalt volcanoes in the Campeche Bay and related sedimentary structures. The leg was split into two parts. During the first sub-leg 2a geophysical and especially hydroacoustic methods were used to explore the distribution of these asphalt volcanoes and to map knolls as well as other structures like mass wasting and asphalt flows. Using reflection seismic, sedimentary structures related to the volcanoes were also investigated. Further mapping but also sampling of vent fluids and asphalt was the research interest of sub-leg 2b. Therefore the Remotely Operating Vehicle (ROV) QUEST (Marum) as well as a TV-MUC were used. Bathymetry mapping was done using the EM120 for deeper and the EM710 for shallower regions. CI Citation: Paul Wintersteller (seafloor-imaging@marum.de) as responsible party for bathymetry raw data ingest and approval. Description of the data source: During the M67/2 cruise, the hull-mounted multibeam echosounder (MBES) KONGSBERG EM710 was utilized to perform bathymetric mapping in shallower areas. It allows to conduct surveys in water depths of up to 2,000 m, however it operates best in shallower water depths under 500 m. Two transducer arrays transmit frequency coded acoustic signals (70 to 100 kHz). Data acquisition is based on continuous wave pulses in shallower depths and FM (chirp) pulses in greater depths. The beam footprint has a dimension of 1° by 1°. For further information on the system, consult: https://www.km.kongsberg.com/ Due to the water depth of the research area the EM120 was permanently used, while the EM710 was only used in few parts of the research area. To convert the recorded travel times into water depth, several sound velocity profiles were obtained with the shipboard CTD, providing a correction for ray bending for each beam. Depth is estimated from each beam by using the two-way travel time and the known beam angle known, and taking into account the ray bending due to refraction in the water column by sound speed variations. Responsible person during this cruise / PI: Volkhard Spieß (vspiess uni-bremen.de), Gerhard Bohrmann (gbohrmann@marum.de) Chief Scientist: : Volkhard Spieß (vspiess uni-bremen.de), Gerhard Bohrmann (gbohrmann@marum.de) CR: https://www.tib.eu/de/suchen/id/awi%3Adoi~10.2312%252Fcr_m67/ CSR: https://www2.bsh.de/aktdat/dod/fahrtergebnis/2006/20060077.htm