ALBERT

Beschreibung: Cell and gene therapy approaches are safer when they possess a system that enables the therapy to be rapidly halted. Human mesenchymal stem cells were transduced with an adenoviral vector containing the cDNA for bone morphogenetic protein 2 (AdBMP2) to induce bone formation. To make this method safer, a system to quickly kill these virally transduced cells was designed and evaluated. Cells were encapsulated inside poly(ethylene glycol) diacrylate (PEG-Da) hydrogels that are able to shield the cells from immunological destruction. The system involves an inducible caspase-9 (iCasp9) activated using a specific chemical inducer of dimerization (CID). Delivering AdBMP2-transduced human mesenchymal stem cells encapsulated in PEG-Da hydrogel promoted ectopic ossification in vivo , and the iCasp9 system allowed direct control of the timing of apoptosis of the injected cells. The iCasp9-CID system enhances the safety of delivering AdBMP2-transduced cells, making it a more compelling therapeutic for bone repair and spine fusion. This article is protected by copyright. All rights reserved

Beschreibung: Author Posting. © American Meteorological Society, 2018. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Climate 31 (2018): 7565-7581, doi:10.1175/JCLI-D-18-0108.1.

Beschreibung: There is mounting evidence that the width of the tropics has increased over the last few decades, but there are large differences in reported expansion rates. This is, likely, in part due to the wide variety of metrics that have been used to define the tropical width. Here we perform a systematic investigation into the relationship among nine metrics of the zonal-mean tropical width using preindustrial control and abrupt quadrupling of CO2 simulations from a suite of coupled climate models. It is shown that the latitudes of the edge of the Hadley cell, the midlatitude eddy-driven jet, the edge of the subtropical dry zones, and the Southern Hemisphere subtropical high covary interannually and exhibit similar long-term responses to a quadrupling of CO2. However, metrics based on the outgoing longwave radiation, the position of the subtropical jet, the break in the tropopause, and the Northern Hemisphere subtropical high have very weak covariations with the above metrics and/or respond differently to increases in CO2 and thus are not good indicators of the expansion of the Hadley cell or subtropical dry zone. The differing variability and responses to increases in CO2 among metrics highlights that care is needed when choosing metrics for studies of the width of the tropics and that it is important to make sure the metric used is appropriate for the specific phenomena and impacts being examined.

Beschreibung: DW acknowledges support from NSF AGS-1403676.

Beschreibung: 2019-02-08

Beschreibung: Abstract The width of the tropical Hadley circulation (HC) has garnered intense interest in recent decades, owing to the emerging evidence for its expansion in observations and models and to the anticipated impacts on surface climate in its descending branches. To better clarify the causes and impacts of tropical widening, this work generalizes the zonal mean HC to the regional level by defining meridional overturning cells (RC) using the horizontally divergent wind. The edges of the RC are more closely connected to surface hydroclimate than more traditional metrics of regional tropical width (such as the sea level pressure ridge) or even than the zonal mean HC. Simulations reveal a robust weakening of the RC in response to greenhouse gas increases, along with a widening of the RC in some regions. For example, simulated widening of the zonal mean HC in the Southern Hemisphere appears to arise in large part from regional overturning anomalies over the Eastern Pacific, where there is no clear RC. Unforced interannual variability in the position of the zonal mean HC edge is associated with a more general regional widening. These distinct regional signatures suggest that the RCs may be well suited for the attribution of observed circulation trends. The spatial pattern of regional meridional overturning trends in reanalyses corresponds more closely to the pattern associated with unforced interannual variability than to the pattern associated with CO2 forcing, suggesting a large contribution of natural variability to the recent observed tropical widening trends.

Beschreibung: Forests, Vol. 9, Pages 422: Toward Sustainable Cultivation of Pinus occidentalis Swartz in Haiti: Effects of Alternative Growing Media and Containers on Seedling Growth and Foliar Chemistry Forests doi: 10.3390/f9070422 Authors: Kyrstan L. Hubbel Amy L. Ross-Davis Jeremiah R. Pinto Owen T. Burney Anthony S. Davis Haiti has suffered great losses from deforestation, with little forest cover remaining today. Current reforestation efforts focus on seedling quantity rather than quality. This study examined limitations to the production of high-quality seedlings of the endemic Hispaniolan pine (Pinus occidentalis Swartz). Recognizing the importance of applying sustainable development principles to pine forest restoration, the effects of growing media and container types on seedling growth were evaluated with the goal of developing a propagation protocol to produce high-quality seedlings using economically feasible nursery practices. With regard to growing media, seedlings grew best in compost-based media amended with sand. Topsoil, widely used in nurseries throughout Haiti, produced the smallest seedlings overall. Despite a low water holding capacity and limited manganese, compost-based media provided adequate levels of essential mineral nutrients (particularly nitrogen), which allowed for sufficient seedling nutrition. Seedling shoot and root growth, as well as the ratio of shoot biomass to root biomass, were greater in polybags relative to D40s. Results indicate that economically feasible improvements to existing nursery practices in Haiti can improve the early growth rates of P. occidentalis seedlings.

Beschreibung: The Antarctic Centennial Oscillation (ACO) is a paleoclimate temperature cycle that originates in the Southern Hemisphere, is the presumptive evolutionary precursor of the contemporary Antarctic Oscillation (AAO), and teleconnects to the Northern Hemisphere to influence global temperature. In this study we investigate the internal climate dynamics of the ACO over the last 21 millennia using stable water isotopes frozen in ice cores from 11 Antarctic drill sites as temperature proxies. Spectral and time series analyses reveal that ACOs occurred at all 11 sites over all time periods evaluated, suggesting that the ACO encompasses all of Antarctica. From the Last Glacial Maximum through the Last Glacial Termination (LGT), ACO cycles propagated on a multicentennial time scale from the East Antarctic coastline clockwise around Antarctica in the streamline of the Antarctic Circumpolar Current (ACC). The velocity of teleconnection (VT) is correlated with the geophysical characteristics of drill sites, including distance from the ocean and temperature. During the LGT, the VT to coastal sites doubled while the VT to inland sites decreased fourfold, correlated with increasing solar insolation at 65°N. These results implicate two interdependent mechanisms of teleconnection, oceanic and atmospheric, and suggest possible physical mechanisms for each. During the warmer Holocene, ACOs arrived synchronously at all drill sites examined, suggesting that the VT increased with temperature. Backward extrapolation of ACO propagation direction and velocity places its estimated geographic origin in the Southern Ocean east of Antarctica, in the region of the strongest sustained surface wind stress over any body of ocean water on Earth. ACO period is correlated with all major cycle parameters except cycle symmetry, consistent with a forced, undamped oscillation in which the driving energy affects all major cycle metrics. Cycle period and symmetry are not discernibly different for the ACO and AAO over the same time periods, suggesting that they are the same climate cycle. We postulate that the ACO/AAO is generated by relaxation oscillation of Westerly Wind velocity forced by the equator-to-pole temperature gradient and propagated regionally by identified air-sea-ice interactions.

Beschreibung: Climate, Vol. 6, Pages 3: The Antarctic Centennial Oscillation: A Natural Paleoclimate Cycle in the Southern Hemisphere That Influences Global Temperature Climate doi: 10.3390/cli6010003 Authors: W. Davis Peter Taylor W. Davis We report a previously-unexplored natural temperature cycle recorded in ice cores from Antarctica—the Antarctic Centennial Oscillation (ACO)—that has oscillated for at least the last 226 millennia. Here we document the properties of the ACO and provide an initial assessment of its role in global climate. We analyzed open-source databases of stable isotopes of oxygen and hydrogen as proxies for paleo-temperatures. We find that centennial-scale spectral peaks from temperature-proxy records at Vostok over the last 10,000 years occur at the same frequencies (±2.4%) in three other paleoclimate records from drill sites distributed widely across the East Antarctic Plateau (EAP), and >98% of individual ACOs evaluated at Vostok match 1:1 with homologous cycles at the other three EAP drill sites and conversely. Identified ACOs summate with millennial periodicity to form the Antarctic Isotope Maxima (AIMs) known to precede Dansgaard-Oeschger (D-O) oscillations recorded in Greenland ice cores. Homologous ACOs recorded at the four EAP drill sites during the last glacial maximum appeared first at lower elevations nearest the ocean and centuries later on the high EAP, with latencies that exceed dating uncertainty >30-fold. ACO homologs at different drill sites became synchronous, however, during the warmer Holocene. Comparative spectral analysis suggests that the millennial-scale AIM cycle declined in period from 1500 to 800 years over the last 70 millennia. Similarly, over the last 226 millennia ACO repetition period (mean 352 years) declined by half while amplitude (mean 0.67 °C) approximately doubled. The period and amplitude of ACOs oscillate in phase with glacial cycles and related surface insolation associated with planetary orbital forces. We conclude that the ACO: encompasses at least the EAP; is the proximate source of D-O oscillations in the Northern Hemisphere; therefore affects global temperature; propagates with increased velocity as temperature increases; doubled in intensity over geologic time; is modulated by global temperature variations associated with planetary orbital cycles; and is the probable paleoclimate precursor of the contemporary Antarctic Oscillation (AAO). Properties of the ACO/AAO are capable of explaining the current global warming signal.

Beschreibung: Subirrigation (SI), where water is provided to container seedlings from below and rises through the growing media via capillary action, is regarded as an environmentally-responsible method of delivering water and fertilizer to nursery-grown plants, resulting in more uniform crops and improved production efficiency. While a concern around adopting this method is that a potential higher salt concentration in the upper layers of growing media under SI may inhibit root growth and result in decreased plant quality, few studies have focused on how root morphology is altered by SI. Therefore, a balanced two-factor factorial design with three rates of fertilization (50, 100, and 150 mg N seedling−1) and two irrigation methods (SI or overhead irrigation (OI)) was used to examine the growth response of Prince Rupprecht’s larch (Larix principis-rupprechtii Mayr) seedlings for one nursery season. Associated changes between rhizosphere electrical conductivity (EC) and root morphology of different root size classes were analyzed. Results show that (1) height, root-collar diameter, and root volume were similar between seedlings grown under SI and OI. However, (2) compared to seedlings receiving OI, SI-seedlings had less root mass, length, and surface area but greater average root diameter (ARD). (3) Morphological differences were evident primarily in root diameter size classes I–III (D ≤ 1.0 mm). (4) Fertilizer rate influenced root length and surface area up to 130 days after sowing but affected ARD throughout the growing season such that seedlings treated with 50 mg N had smaller ARD than seedlings treated with 100 mg N. (5) As the growing season progressed, SI-media had significantly higher EC compared to OI-media and EC increased with increasing fertilizer rate under SI but not under OI. These results indicate that SI can produce larch seedlings of similar height and root collar diameter (RCD) compared to OI, but root systems are smaller overall with fewer small-diameter roots, which may be related to high EC levels in SI-media, which is exacerbated by the use of high rates of fertilizer. Therefore, the EC in the media should be monitored and adjusted by reducing fertilizer rates under SI.