ALBERT

Description: We investigated Legionella contamination in bath water samples, collected from 68 bathing facilities in Japan, by culture, culture with amoebic co-culture, real-time quantitative PCR (qPCR), and real-time qPCR with amoebic co-culture. Using the conventional culture method, Legionella pneumophila was detected in 11 samples (11/68, 16.2%). Contrary to our expectation, the culture method with the amoebic co-culture technique did not increase the detection rate of Legionella (4/68, 5.9%). In contrast, a combination of the amoebic co-culture technique followed by qPCR successfully increased the detection rate (57/68, 83.8%) compared with real-time qPCR alone (46/68, 67.6%). Using real-time qPCR after culture with amoebic co-culture, more than 10-fold higher bacterial numbers were observed in 30 samples (30/68, 44.1%) compared with the same samples without co-culture. On the other hand, higher bacterial numbers were not observed after propagation by amoebae in 32 samples (32/68, 47.1%). Legionella was not detected in the remaining six samples (6/68, 8.8%), irrespective of the method. These results suggest that application of the amoebic co-culture technique prior to real-time qPCR may be useful for the sensitive detection of Legionella from bath water samples. Furthermore, a combination of amoebic co-culture and real-time qPCR might be useful to detect viable and virulent Legionella because their ability to invade and multiply within free-living amoebae is considered to correlate with their pathogenicity for humans. This is the first report evaluating the efficacy of the amoebic co-culture technique for detecting Legionella in bath water samples.

Description: Recently, we developed novel chiral dendrimer-triamine-coordinated Gd-MRI contrast agents (Gd-MRI CAs), which showed longitudinal relaxivity (r1) values about four times higher than that of clinically used Gd-DTPA (Magnevist®, Bayer). In our continuing study of pharmacokinetic differences derived from both the chirality and generation of Gd-MRI CAs, we found that the ability of chiral dendrimer Gd-MRI CAs to circulate within the body can be directly evaluated by in vitro MRI (7 T). In this study, the association constants (Ka) of chiral dendrimer Gd-MRI CAs to bovine serum albumin (BSA), measured and calculated with a quartz crystal microbalance (QCM) in vitro, were found to be an extremely easy means for evaluating the body-circulation ability of chiral dendrimer Gd-MRI CAs. The Ka values of S-isomeric dendrimer Gd-MRI CAs were generally greater than those of R-isomeric dendrimer Gd-MRI CAs, which is consistent with the results of our previous MRI study in vivo.

Description: Although lead-free piezoelectric ceramics have been extensively studied, many problems must still be overcome before they are suitable for practical use. One of the main problems is fabricating a multilayer structure, and one solution attracting growing interest is the use of lead-free multilayer piezoelectric ceramics. The paper reviews work that has been done by the authors on lead-free alkali niobate-based multilayer piezoelectric ceramics co-fired with nickel inner electrodes. Nickel inner electrodes have many advantages, such as high electromigration resistance, high interfacial strength with ceramics, and greater cost effectiveness than silver palladium inner electrodes. However, widely used lead zirconate titanate-based ceramics cannot be co-fired with nickel inner electrodes, and silver palladium inner electrodes are usually used for lead zirconate titanate-based piezoelectric ceramics. A possible alternative is lead-free ceramics co-fired with nickel inner electrodes. We have thus been developing lead-free alkali niobate-based multilayer ceramics co-fired with nickel inner electrodes. The normalized electric-field-induced thickness strain (Smax/Emax) of a representative alkali niobate-based multilayer ceramic structure with nickel inner electrodes was 360 pm/V, where Smax denotes the maximum strain and Emax denotes the maximum electric field. This value is about half that for the lead zirconate titanate-based ceramics that are widely used. However, a comparable value can be obtained by stacking more ceramic layers with smaller thicknesses. In the paper, the compositional design and process used to co-fire lead-free ceramics with nickel inner electrodes are introduced, and their piezoelectric properties and reliabilities are shown. Recent advances are introduced, and future development is discussed.

Description: Geosciences, Vol. 8, Pages 359: Coupled Model of Bank Erosion and Meander Evolution for Cohesive Riverbanks Geosciences doi: 10.3390/geosciences8100359 Authors: Kattia Rubi Arnez Ferrel Supapap Patsinghasanee Ichiro Kimura Yasuyuki Shimizu In this paper, a physics-based model that couples a bank erosion model with a meander evolution model is developed and evaluated. The physics-based bank erosion model considers the cantilever failure mechanism with slump blocks and decomposition effects. Moreover, bank accretion was considered using critical values of time required for landing, shear stresses and water depths. Two cases were tested. The first case consists of a hypothetical small-scale channel with cohesive riverbanks. Cross sections in the straight and curved part of the channel were compared to evaluate the curvature effect. Furthermore, the effect of the bank strength in the plan shape of the channel was tested in this case. The results show that the curvature increases the erosion rate in the outer bank and changes the cross-sectional profile by narrowing and widening the channel width. The plan shape of the channel changed as the bank strength was increased. In the second case, the model is compared with the River meander migration software (RVR meander) and the advantages and limitations of the model are discussed in terms of meander migration plan form and bank erosion processes. The results showed that the presented model is capable of simulating asymmetric bends.

Description: Sensors, Vol. 18, Pages 2895: An Integrated Dead Reckoning with Cooperative Positioning Solution to Assist GPS NLOS Using Vehicular Communications Sensors doi: 10.3390/s18092895 Authors: Pedro Paulo Liborio Lima do Nascimento Bruno Yuji Lino Kimura Daniel Ludovico Guidoni Leandro Aparecido Villas In Intelligent Transportation Systems (ITS), the Vehicular Ad Hoc Networks (VANETs) paradigm based on the WAVE IEEE 802.11p standard is the main alternative for inter-vehicle communications. Recently, many protocols, applications, and services have been developed with a wide range of objectives, ranging from comfort to security. Most of these services rely on location systems and require different levels of accuracy for their full operation. The Global Positioning System (GPS) is an off-the-shelf solution for localization in VANETs and ITS. However, GPS systems present problems regarding inaccuracy and unavailability in dense urban areas, multilevel roads, and tunnels, posing a challenge for protocols, applications, and services that rely on localization. With this motivation, we carried out a characterization of the problems of inaccuracy and unavailability of GPS systems from real datasets, and regions around tunnels were selected. Since the nodes of the vehicular network are endowed with wireless communication, processing and storage capabilities, an integrated Dead Reckoning aided Geometric Dilution of Precision (GDOP)-based Cooperative Positioning solution was developed and evaluated. Leveraging the potential of vehicular sensors, such as odometers, gyroscopes, and digital compasses, vehicles share their positions and kinematics information using vehicular communication to improve their location estimations. With the assistance of a digital map, vehicles adjust the final estimated position using the road geometry. The situations of GPS unavailability characterized in the datasets were reproduced in a simulation environment to validate the proposed localization solution. The simulation results show average gains in Root Mean Square Error (RMSE) between 97% to 98% in comparison with the stand-alone GPS solution, and 83.00% to 88.00% against the GPS and Dead Reckoning (DR) only solution. The average absolute RMSE was reduced to the range of 3 to 5 m by vehicle. In addition, the proposed solution was shown to support 100% of the GPS unavailability zones on the evaluated scenarios.

Description: Water, Vol. 10, Pages 1245: Describing and Visualizing a Water–Energy–Food Nexus System Water doi: 10.3390/w10091245 Authors: Aiko Endo Terukazu Kumazawa Michinori Kimura Makoto Yamada Takaaki Kato Kouji Kozaki The objective of this study is to describe a target water–energy–food (WEF) nexus domain world including causal linkages and trade-off relationships between WEF resources and their stakeholders, and to develop a WEF nexus system map as an interdisciplinary tool used for understanding the subsequent complexity of WEF nexus systems. An ontology engineering method, which is a qualitative method, was applied for the replicability of the WEF nexus domain ontology and the map, because ontology engineering is a method of semantic web development for enhancing the compatibility of qualitative descriptions logically or objectively. The WEF nexus system map has three underlying concepts: (1) systems thinking, (2) holistic thinking, and (3) an integrated approach at an operational level, according to the hypothesis that the chains of changes in linkages between water, energy, and food resources holistically and systemically affect the WEF nexus system, including natural and social systems, both temporally and spatially. This study is significant because it allows us to (1) develop the WEF nexus domain ontology database, including defining the concepts and sub-concepts of trade-offs relating to WEF for the replicability of this study; (2) integrate the qualitative ontology method and quantitative network analysis method to identify key concepts serving as linkage hubs in the WEF nexus domain ontology; and (3) visualize human–nature interactions such as linkages between water, energy, and food resources and their stakeholders in social and natural systems. This paper also discusses future challenges in the application of the map for a science–policy–society interface.

Description: Atmosphere, Vol. 9, Pages 356: Comparison of Closed Chamber and Eddy Covariance Methods to Improve the Understanding of Methane Fluxes from Rice Paddy Fields in Japan Atmosphere doi: 10.3390/atmos9090356 Authors: Nongpat Chaichana Sonoko Dorothea Bellingrath-Kimura Shujiro Komiya Yoshiharu Fujii Kosuke Noborio Ottfried Dietrich Tiwa Pakoktom Greenhouse gas flux monitoring in ecosystems is mostly conducted by closed chamber and eddy covariance techniques. To determine the relevance of the two methods in rice paddy fields at different growing stages, closed chamber (CC) and eddy covariance (EC) methods were used to measure the methane (CH4) fluxes in a flooded rice paddy field. Intensive monitoring using the CC method was conducted at 30, 60 and 90 days after transplanting (DAT) and after harvest (AHV). An EC tower was installed at the centre of the experimental site to provide continuous measurements during the rice cropping season. The CC method resulted in CH4 flux averages that were 58%, 81%, 94% and 57% higher than those measured by the EC method at 30, 60 and 90 DAT and after harvest (AHV), respectively. A footprint analysis showed that the area covered by the EC method in this study included non-homogeneous land use types. The different strengths and weaknesses of the CC and EC methods can complement each other, and the use of both methods together leads to a better understanding of CH4 emissions from paddy fields.

Description: Water, Vol. 10, Pages 843: The Impact of Multiple Typhoons on Severe Floods in the Mid-Latitude Region (Hokkaido) Water doi: 10.3390/w10070843 Authors: Nobuaki Kimura Hirohide Kiri Iwao Kitagawa Mid-latitude regions in the North Pacific are generally vulnerable to climatological disasters and are possibly more sensitive to future climate changes. Severe flood disasters struck Hokkaido in August 2016 because of the multiple, continuous typhoons that struck the island. We evaluated the effect of these typhoons on floods and changes in future floods using a distributed hydrological model in a watershed located in eastern Hokkaido. We conducted two numerical examinations: a simulation with a major typhoon only (which caused flood disasters) without other preceding typhoons, and a simulation with a simple assumed future climate (in which we employed higher precipitation). The result of the former simulation demonstrated that the impact of the preceding typhoons on the highest flood peak was significant during the early stage of the major typhoon but weaker after the middle stage of the major typhoon. The result of the latter simulation indicated that flood peaks potentially increased with an increase in precipitation. Based on the water level distributions in the surface layer, the impact of multiple typhoons and future weather conditions on potential flood peaks depends on the degree of soil saturation over our target watershed.

Description: Sustainability, Vol. 10, Pages 1034: Exploring Farmers’ Indigenous Knowledge of Soil Quality and Fertility Management Practices in Selected Farming Communities of the Guinea Savannah Agro-Ecological Zone of Ghana Sustainability doi: 10.3390/su10041034 Authors: Richard Ansong Omari Sonoko Bellingrath-Kimura Elsie Sarkodee Addo Yosei Oikawa Yoshiharu Fujii Efforts to improve soil productive capacity aimed at boosting crop production in the Northern Ghana has primarily focused on field-based experiments with little documentation on farmer practice and local indigenous knowledge of soil management. A sample group of 114 farmers from five farming communities in the Guinea Savannah was interviewed to evaluate their indigenous knowledge of crop production practices in the context of soil health, fertilization management, and crop yield. Data were collected using semi-structured interviews and responses for each category were calculated using simple proportions. Farmers’ fertilization practice was primarily influenced by fertilization resource availability and crop yield response. The results showed that inorganic fertilization was the commonest fertilization type among farmers. Farmer local indicators of soil health were predominantly limited to visually observable signs such as presence or absence of indicator plants, growth vigor of plants, soil color, and tilth, texture, and compaction. Non-tactile and visible indicators, notably soil chemical composition and presence of soil microorganisms, was rarely used. The listed indicators were congruent with scientific reports, although some knowledge gaps, particularly on the use of indicator plants, were identified. The use of indicator plants as determinants of healthy or non-healthy soils appeared to be influenced by the ease of control of weeds, its utilitarian benefits, benefits to the soil, and threats on cultivated crops. Famers were well informed about the decreasing crop yield. Fertilization practices and limitations in soil management practices with proposed capacity building approaches aimed at enhancing productive capacities of cultivated farmlands are discussed.