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Coincidence of a high-fluence blazar outburst with a PeV-energy neutrino event (2016)

M. Kadler; F. KraußK. Mannheim; R. Ojha; C. Müller; R. Schulz; G. Anton; W. Baumgartner; T. Beuchert; S. Buson; B. Carpenter; T. Eberl; P. G. Edwards; D. Eisenacher Glawion; D. Elsässer; N. Gehrels; C. Gräfe; S. Gulyaev; H. Hase; S. Horiuchi; C. W. James; A. Kappes; A. Kappes; U. Katz; A. Kreikenbohm; M. Kreter; I. Kreykenbohm; M. Langejahn; K. Leiter; E. Litzinger; F. Longo; J. E. J. Lovell; J. McEnery; T. Natusch; C. Phillips; C. Plötz; J. Quick; E. Ros; F. W. Stecker; T. Steinbring; J. Stevens; D. J. Thompson; J. Trüstedt; A. K. Tzioumis; S. Weston; J. Wilms; J. A. Zensus

Springer Nature

In: Nature Physics

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Publikationsdatum: 2016-08-03

Beschreibung: Nature Physics 12, 807 (2016). doi:10.1038/nphys3715 Authors: M. Kadler, F. Krauß, K. Mannheim, R. Ojha, C. Müller, R. Schulz, G. Anton, W. Baumgartner, T. Beuchert, S. Buson, B. Carpenter, T. Eberl, P. G. Edwards, D. Eisenacher Glawion, D. Elsässer, N. Gehrels, C. Gräfe, S. Gulyaev, H. Hase, S. Horiuchi, C. W. James, A. Kappes, A. Kappes, U. Katz, A. Kreikenbohm, M. Kreter, I. Kreykenbohm, M. Langejahn, K. Leiter, E. Litzinger, F. Longo, J. E. J. Lovell, J. McEnery, T. Natusch, C. Phillips, C. Plötz, J. Quick, E. Ros, F. W. Stecker, T. Steinbring, J. Stevens, D. J. Thompson, J. Trüstedt, A. K. Tzioumis, S. Weston, J. Wilms & J. A. Zensus

Print ISSN: 1745-2473

Digitale ISSN: 1745-2481

Thema: Physik

Publiziert von Springer Nature

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A new fungal large subunit ribosomal RNA primer for high-throughput sequencing surveys (2016)

Mueller, R. C., Gallegos-Graves, L. V., Kuske, C. R.

Oxford University Press

In: FEMS Microbiology Ecology

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Publikationsdatum: 2016-01-31

Beschreibung: The inclusion of phylogenetic metrics in community ecology has provided insights into important ecological processes, particularly when combined with high-throughput sequencing methods; however, these approaches have not been widely used in studies of fungal communities relative to other microbial groups. Two obstacles have been considered: (1) the internal transcribed spacer (ITS) region has limited utility for constructing phylogenies and (2) most PCR primers that target the large subunit (LSU) ribosomal unit generate amplicons that exceed current limits of high-throughput sequencing platforms. We designed and tested a PCR primer (LR22R) to target approximately 300–400 bp region of the D2 hypervariable region of the fungal LSU for use with the Illumina MiSeq platform. Both in silico and empirical analyses showed that the LR22R–LR3 pair captured a broad range of fungal taxonomic groups with a small fraction of non-fungal groups. Phylogenetic placement of publically available LSU D2 sequences showed broad agreement with taxonomic classification. Comparisons of the LSU D2 and the ITS2 ribosomal regions from environmental samples and known communities showed similar discriminatory abilities of the two primer sets. Together, these findings show that the LR22R–LR3 primer pair has utility for phylogenetic analyses of fungal communities using high-throughput sequencing methods.

Print ISSN: 0168-6496

Digitale ISSN: 1574-6941

Thema: Biologie

Publiziert von Oxford University Press

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Fast and precise large area metrology of micropattern detectors using laser distance sensors (2016)

Müller, R. ; Biebel, O. ; Hertenberger, R. ; [weitere]

Elsevier

In: Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment. 2016; 824: 215-217. Published 2016 Jul 01. doi: 10.1016/j.nima.2015.11.003.

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Publikationsdatum: 2016-07-01

Print ISSN: 0168-9002

Digitale ISSN: 1872-9576

Thema: Physik

Publiziert von Elsevier

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On the thermal stability of electrodeposited Ni-matrix-nanocomposite films reinforced with Ag-coated SnO2 nanowires (2016)

Miguel, F.L. ; Müller, R. ; Mathur, S. ; [weitere]

Elsevier

In: Surface and Coatings Technology. 2016; 287: 93-102. Published 2016 Feb 01. doi: 10.1016/j.surfcoat.2015.12.085.

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Publikationsdatum: 2016-02-01

Print ISSN: 0257-8972

Digitale ISSN: 1879-3347

Thema: Maschinenbau , Physik

Publiziert von Elsevier

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Two N-terminally truncated variants of human {beta}-galactoside {alpha}2,6 sialyltransferase I with distinct properties for in vitro protein glycosylation (2016)

Luley-Goedl, C., Schmoelzer, K., Thomann, M., Malik, S., Greif, M., Ribitsch, D., Jung, C., Sobek, H., Engel, A., Mueller, R., Schwab, H., Nidetzky, B.

Oxford University Press

In: Glycobiology

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Publikationsdatum: 2016-10-20

Beschreibung: Sialic acid groups of protein N -glycans are important determinants of biological activity. Exposed at the end of the glycan chain, they are potential targets for glycan remodeling. Sialyltransferases (STs; EC 2.4.99) are the enzymes that catalyze the sialic acid transfer from a CMP-activated donor on to a carbohydrate acceptor in vivo. Recombinant expression of the full-length human β-galactoside α2,6 sialyltransferase I (ST6Gal-I) was hampered and therefore variants with truncated N-termini were investigated. We report on the distinct properties of two N-terminally truncated versions of ST6Gal-I, namely 89ST6Gal-I and 108ST6Gal-I, which were successfully expressed in human embryonic kidney cells. The different properties of these enzymes result most probably from the loss of interactions from helix α1 in the 108ST6Gal-I variant, which plays a role in acceptor substrate binding. The K m for N -acetyl- d -lactosamine was 10-fold increased for 108ST6Gal-I (84 mM) as compared to 89ST6Gal-I (8.3 mM). The two enzyme variants constitute a suitable tool box for the terminal modification of N -glycans. While the enzyme 89ST6Gal-I exhibited both ST (di-sialylation) and sialidase activity on a monoclonal antibody, the enzyme 108ST6Gal-I showed only ST activity with specificity for mono-sialylation.

Print ISSN: 0959-6658

Digitale ISSN: 1460-2423

Thema: Biologie , Medizin

Publiziert von Oxford University Press

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