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  • Hindawi  (18)
  • BioMed Central  (6)
  • Sage Publications  (4)
  • MDPI Publishing  (1)
  • American Association for the Advancement of Science
  • 2015-2019
  • 2010-2014  (29)
  • 2000-2004
  • 2014  (29)
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  • 2015-2019
  • 2010-2014  (29)
  • 2000-2004
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  • 1
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    Molecular characterization of KU70 and KU80 homologues and exploitation of a KU70-deficient mutant for improving gene deletion frequency in Rhodosporidium toruloides (2014)
    BioMed Central
    Details
    Publication Date: 2014-02-28
    Description: Background: Rhodosporidium toruloides is a beta-carotenoid accumulating, oleaginous yeast that has great biotechnological potential. The lack of reliable and efficient genetic manipulation tools have been a major hurdle blocking its adoption as a biotechnology platform. Results: We report for the first time the development of a highly efficient targeted gene deletion method in R. toruloides ATCC 10657 via Agrobacterium tumefaciens-mediated transformation. To further improve targeting frequency, the KU70 and KU80 homologs in R. toruloides were isolated and characterized in detail. A KU70-deficient mutant ([increment]ku70e) generated with the hygromycin selection cassette removed by the Cre-loxP recombination system showed a dramatically improved targeted gene deletion frequency, with over 90% of the transformants being true knockouts when homology sequence length of at least 1 kb was used. Successful gene targeting could be made with homologous flanking sequences as short as 100 bp in the [increment]ku70e strain. KU70 deficiency did not perturb cell growth although an elevated sensitivity to DNA mutagenic agents was observed. Compared to the other well-known oleaginous yeast, Yarrowia lipolytica, R. toruloides KU70/KU80 genes contain much higher density of introns and are the most GC-rich KU70/KU80 genes reported. Conclusions: The KU70-deficient mutant generated herein was effective in improving gene deletion frequency and allowed shorter homology sequences to be used for gene targeting. It retained the key oleaginous and fast growing features of R. toruloides. The strain should facilitate both fundamental and applied studies in this important yeast, with the approaches taken here likely to be applicable in other species in subphylum Pucciniomycotina.
    Electronic ISSN: 1471-2180
    Topics: Biology
    Published by BioMed Central
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  • 2
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    Stochastic Viscoelastic Wave Equations with Nonlinear Damping and Source Terms (2014)
    Hindawi
    Details
    Publication Date: 2014-03-28
    Description: The goal of this paper is to study an initial boundary value problem of stochastic viscoelastic wave equation with nonlinear damping and source terms. Under certain conditions on the initial data: the relaxation function, the indices of nonlinear damping, and source terms and the random force, we prove the local existence and uniqueness of solution by the Galerkin approximation method. Then, considering the relationship between the indices of nonlinear damping and nonlinear source, we give the necessary conditions of global existence and explosion in finite time in some sense of solutions, respectively.
    Print ISSN: 1110-757X
    Electronic ISSN: 1687-0042
    Topics: Mathematics
    Published by Hindawi
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  • 3
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    A survey of plant and algal genomes and transcriptomes reveals new insights into the evolution and function of the cellulose synthase superfamily (2014)
    BioMed Central
    Details
    Publication Date: 2014-04-05
    Description: Background: Enzymes of the cellulose synthase (CesA) family and CesA-like (Csl) families are responsible for the synthesis of celluloses and hemicelluloses, and thus are of great interest to bioenergy research. We studied the occurrences and phylogenies of CesA/Csl families in diverse plants and algae by comprehensive data mining of 82 genomes and transcriptomes. Results: We found that 1) charophytic green algae (CGA) have orthologous genes in CesA, CslC and CslD families; 2) liverwort genes are found in the CesA, CslA, CslC and CslD families; 3) The fern Pteridium aquilinum not only has orthologs in these conserved families but also in the CslB, CslH and CslE families; 4) basal angiosperms, e.g. Aristolochia fimbriata, have orthologs in these families too; 5) gymnosperms have genes forming clusters ancestral to CslB/H and to CslE/J/G respectively; 6) CslG is found in switchgrass and basal angiosperms; 7) CslJ is widely present in dicots and monocots; 8) CesA subfamilies have already diversified in ferns. Conclusions: We speculate that: (i) ferns and horsetails might both have CslH enzymes, responsible for the synthesis of mixed-linkage glucans and (ii) CslD and similar genes might be responsible for the synthesis of mannans in CGA. Our findings led to a more detailed model of cell wall evolution and suggested that gene loss played an important role in the evolution of Csl families. We also demonstrated the usefulness of transcriptome data in the study of plant cell wall evolution and diversity.
    Electronic ISSN: 1471-2164
    Topics: Biology
    Published by BioMed Central
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  • 4
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    Sensors, Vol. 14, Pages 9210-9226: Comparative Study on a Solving Model and Algorithm for a Flush Air Data Sensing System (2014)
    MDPI Publishing
    In: Sensors
    Details
    Publication Date: 2014-05-24
    Description: With the development of high-performance aircraft, precise air data are necessary to complete challenging tasks such as flight maneuvering with large angles of attack and high speed. As a result, the flush air data sensing system (FADS) was developed to satisfy the stricter control demands. In this paper, comparative stuides on the solving model and algorithm for FADS are conducted. First, the basic principles of FADS are given to elucidate the nonlinear relations between the inputs and the outputs. Then, several different solving models and algorithms of FADS are provided to compute the air data, including the angle of attck, sideslip angle, dynamic pressure and static pressure. Afterwards, the evaluation criteria of the resulting models and algorithms are discussed to satisfy the real design demands. Futhermore, a simulation using these algorithms is performed to identify the properites of the distinct models and algorithms such as the measuring precision and real-time features. The advantages of these models and algorithms corresponding to the different flight conditions are also analyzed, furthermore, some suggestions on their engineering applications are proposed to help future research.
    Electronic ISSN: 1424-8220
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Published by MDPI Publishing
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  • 5
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    Invariant Hough Random Ferns for Object Detection and Tracking (2014)
    Hindawi
    Details
    Publication Date: 2014-04-09
    Description: This paper introduces an invariant Hough random ferns (IHRF) incorporating rotation and scale invariance into the local feature description, random ferns classifier training, and Hough voting stages. It is especially suited for object detection under changes in object appearance and scale, partial occlusions, and pose variations. The efficacy of this approach is validated through experiments on a large set of challenging benchmark datasets, and the results demonstrate that the proposed method outperforms state-of-the-art conventional methods such as bounding-box-based and part-based methods. Additionally, we also propose an efficient clustering scheme based on the local patches’ appearance and their geometric relations that can provide pixel-accurate, top-down segmentations from IHRF back-projections. This refined segmentation can be used to improve the quality of online object tracking because it avoids the drifting problem. Thus, an online tracking framework based on IHRF, which is trained and updated in each frame to distinguish and segment the object from the background, is established. Finally, the experimental results on both object segmentation and long-term object tracking show that this method yields accurate and robust tracking performance in a variety of complex scenarios, especially in cases of severe occlusions and nonrigid deformations.
    Print ISSN: 1024-123X
    Electronic ISSN: 1563-5147
    Topics: Mathematics , Technology
    Published by Hindawi
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  • 6
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    Genome sequence and transcriptome analyses of the thermophilic zygomycete fungus Rhizomucor miehei (2014)
    BioMed Central
    Details
    Publication Date: 2014-04-22
    Description: Background: The zygomycete fungi like Rhizomucor miehei have been extensively exploited for the production of various enzymes. As a thermophilic fungus, R. miehei is capable of growing at temperatures that approach the upper limits for all eukaryotes. To date, over hundreds of fungal genomes are publicly available. However, Zygomycetes have been rarely investigated both genetically and genomically. Results: Here, we report the genome of R. miehei CAU432 to explore the thermostable enzymatic repertoire of this fungus. The assembled genome size is 27.6-million-base (Mb) with 10,345 predicted protein-coding genes. Even being thermophilic, the G + C contents of fungal whole genome (43.8%) and coding genes (47.4%) are less than 50%. Phylogenetically, R. miehei is more closerly related to Phycomyces blakesleeanus than to Mucor circinelloides and Rhizopus oryzae. The genome of R. miehei harbors a large number of genes encoding secreted proteases, which is consistent with the characteristics of R. miehei being a rich producer of proteases. The transcriptome profile of R. miehei showed that the genes responsible for degrading starch, glucan, protein and lipid were highly expressed. Conclusions: The genome information of R. miehei will facilitate future studies to better understand the mechanisms of fungal thermophilic adaptation and the exploring of the potential of R. miehei in industrial-scale production of thermostable enzymes. Based on the existence of a large repertoire of amylolytic, proteolytic and lipolytic genes in the genome, R. miehei has potential in the production of a variety of such enzymes.
    Electronic ISSN: 1471-2164
    Topics: Biology
    Published by BioMed Central
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  • 7
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    An Attribute-Based Encryption Scheme with Revocation for Fine-Grained Access Control in Wireless Body Area Networks (2014)
    Hindawi
    Details
    Publication Date: 2014-11-12
    Description: The wireless body area networks (WBANs) have emerged as a new method for e-healthcare. Without being measured face-to-face, the medical workers can give guidance to patients in a real-time way. WBANs can greatly improve the healthcare quality. The personal information and medical data are stored and processed in sensors. The security and privacy are two vital issues. In this paper, we design an attribute-based encryption scheme for fine-grained access control in WBANs. In our scheme, a user can decrypt a ciphertext if the attributes related with a ciphertext satisfy the user’s access structure. The users can be revoked if necessary. Therefore, the security and privacy of patients can be protected. Our scheme provides confidentiality, security, and resistance to collusion attack. We analyze the correctness, security, and energy consumption of the scheme.
    Print ISSN: 1550-1329
    Electronic ISSN: 1550-1477
    Topics: Electrical Engineering, Measurement and Control Technology
    Published by Hindawi
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  • 8
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    Mathematical Modeling of Eddy-Current Loss for a New Induction Heating Device (2014)
    Hindawi
    Details
    Publication Date: 2014-06-02
    Description: A new induction heating device is presented in this paper. This device can convert mechanical energy into heat energy by utilizing eddy currents, which are induced by rotating permanent magnets. A mathematical model is established for estimating eddy-current loss of the device. The distribution of induced currents and the resultant magnetic field intensity are considered in the process of modeling the eddy-current loss and so is the mutual influence of the electric field between neighborhood pole projection areas. Particularly, the skin effect is considered by correcting the numerical integral domain of eddy current density, which has great effect on the calculating results. Based on specific examples, the effectiveness and correctness of proposed model are proved by finite element analysis. The results show that the mathematical model can provide important reference for design and structure optimization of the device.
    Print ISSN: 1024-123X
    Electronic ISSN: 1563-5147
    Topics: Mathematics , Technology
    Published by Hindawi
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  • 9
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    Blind Channel Estimation Based on Multilevel Lloyd-Max Iteration for Nonconstant Modulus Constellations (2014)
    Hindawi
    Details
    Publication Date: 2014-07-21
    Description: In wireless communications, knowledge of channel coefficients is required for coherent demodulation. Lloyd-Max iteration is an innovative blind channel estimation method for narrowband fading channels. In this paper, it is proved that blind channel estimation based on single-level Lloyd-Max (SL-LM) iteration is not reliable for nonconstant modulus constellations (NMC). Then, we introduce multilevel Lloyd-Max (ML-LM) iteration to solve this problem. Firstly, by dividing NMC into subsets, Lloyd-Max iteration is used in multilevel. Then, the estimation information is transmitted from one level to another. By doing this, accurate blind channel estimation for NMC is achieved. Moreover, when the number of received symbols is small, we propose the lacking constellations equalization algorithm to reduce the influence of lacking constellations. Finally, phase ambiguity of ML-LM iteration is also investigated in the paper. ML-LM iteration can be more robust to the phase of fading coefficient by dividing NMC into subsets properly. As the signal-to-noise ratio (SNR) increases, numerical results show that the proposed method’s mean-square error curve converges remarkably to the least squares (LS) bound with a small number of iterations.
    Print ISSN: 1110-757X
    Electronic ISSN: 1687-0042
    Topics: Mathematics
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  • 10
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    Prediction of Water Consumption in Hospitals Based on a Modified Grey GM (0, 1∣sin) Model of Oscillation Sequence: The Example of Wuhan City (2014)
    Hindawi
    Details
    Publication Date: 2014-07-04
    Description: Water shortage is one of the main factors limiting urban construction and development. Scientific forecasting of water consumption is an important approach for the rational allocation of water resources. Taking the hospitals in Wuhan City as an example and basing the analysis on the characteristics of actual water consumption, we proposed a modified grey GM (0, 1∣sin) model of oscillation sequence. Using the grey theory, the variable weight-strengthening buffer operator (VWSBO) was introduced into this model to weaken the interference of the disturbance term on the data sequence. The actual quarterly total water consumption data for hospitals in Wuhan City during the period from 2010 to 2012 were used to verify the effectiveness and practicality of this modified grey GM (0, 1∣sin) model in predicting water consumption. In terms of the model’s fitting performance, the mean absolute percentage error (MAPE) of the modified model was 3.77%, indicating a higher prediction accuracy than the traditional grey GM (0, 1∣sin) model of oscillation sequences. Therefore, the modified grey GM (0, 1∣sin) model we established in this study can provide a scientific reference for administrative departments to forecast water consumption.
    Print ISSN: 1110-757X
    Electronic ISSN: 1687-0042
    Topics: Mathematics
    Published by Hindawi
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