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  • 1
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: 1. The effects of phosphorus enrichment and grazing snails on a benthic microbial community that builds stromatolic oncolites were examined in an experiment at Rio Mesquites, Cuatro Ciénegas, Mexico. Chemical analyses of stream water samples indicated that overall atomic ratios of total nitrogen (N) to total phosphorus (P) were approximately 110, indicating a strong potential for P-limitation of microbial growth.2. Phosphorus enrichment involved addition of 5 μmol Na2HPO4 L−1 to streamside microcosms receiving intermittent inputs of stream water while grazer manipulation involved removal of the dominant grazer, the snail Mexithauma quadripaludium. After 7 weeks, we examined responses in organic matter content, C : N : P ratios, metabolism (P removal, primary production, dark respiration, and calcification), and microbial community structure using molecular fingerprinting of 16S rRNA genes.3. Manipulation of snails did not affect response variables measured in these treatments (organic matter, C : P ratio, P removal rate). However, P enrichment significantly decreased the C : P and N : P ratios of surficial materials in the oncolites (organic matter content was unchanged), increased net and gross photosynthesis (oxygen consumption in the dark was unchanged), increased rates of calcification, and increased diatoms relative to cyanobacteria. Heterotrophic Eubacteria and Archaea were only modestly affected. Thus, our results indicate weak grazing effects but strong impacts of P in this benthic system.4. We hypothesise that a state of severe P-limitation is imposed on autotrophic production in this food web due, at least in part, to co-precipitation of phosphate during calcite deposition. This produces severe P-limitation of the benthic algae and cyanobacteria, resulting in high C : P ratio of microbial mats relative to the biomass of photoautotrophs (phytoplankton, terrestrial foliage) in other ecosystems. In turn, this high C : P ratio is likely to generate severe stoichiometric constraints on the herbivores, thus limiting their populations and resulting in weak overall grazing impacts.
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  • 2
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: 1. Sedimentary remains of aquatic plants, both vegetative (turions, leaves, spines) and reproductive (fruits, seeds, pollen), may provide a record of temporal changes in the submerged vegetation of lakes. An independent assessment of the degree to which these remains reflect past floristic change is, however, rarely possible.2. By exploiting an extensive series of historical plant records for a small shallow lake we compare plant macrofossil (three cores) and pollen (one core) profiles with the documented sequence of submerged vegetation change since c. 1750 AD. The data set is based on 146 site visits with 658 observations including 42 taxa classified as aquatic, spanning 250 years.3. Approximately 40% of the historically recorded aquatic taxa were represented by macro-remains. In general macrofossils underestimated past species diversity, with pondweeds (three of eight historically recorded Potamogeton species were found) particularly poorly represented. Nonetheless, several taxa not reported from historical surveys (e.g. Myriophyllum alterniflorum and Characeae) were present in the sediment record.4. The pollen record revealed taxa which left no macro-remains (e.g. Littorella uniflora), and the macrofossil record provided improved taxonomic resolution for some taxa (e.g. Potamogeton) and a more reliable record of persistence, appearance and loss of others (e.g. Myriophyllum spp. and Nymphaeaceae).5. Detrended correspondence analysis indicated that changes in the community composition evidenced by the palaeolimnological and historical records were synchronous and of a similar magnitude. Both records pointed to a major change at around 1800, with the historical record suggesting a more abrupt change than the sedimentary data. There was good agreement on a subsequent change c. 1930.6. The palaeolimnological data did not provide a complete inventory of historically recorded species. Nevertheless, these results suggest that combined macrofossil and pollen records provide a reliable indication of temporal change in the dominant components of the submerged and floating-leaved aquatic vegetation of shallow lakes. As such palaeolimnology may provide a useful tool for establishing community dynamics and successions of plants over decadal to centennial timescales.
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  • 3
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Microbiology 59 (2005), S. 487-517 
    ISSN: 0066-4227
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Notes: Riboswitches are structured domains that usually reside in the noncoding regions of mRNAs, where they bind metabolites and control gene expression. Like their protein counterparts, these RNA gene control elements form highly specific binding pockets for the target metabolite and undergo allosteric changes in structure. Numerous classes of riboswitches are present in bacteria and they comprise a common and robust metabolite-sensing system.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the World Aquaculture Society 36 (2005), S. 0 
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: An economic analysis of a hypothetical small-scale marine recirculating aquaculture system (RAS) is conducted for ongrowing small, wild black sea bass Centropristis striata at the University of North Carolina Wilmington, Center for Marine Science (UNCW-CMS) aquaculture facility in Wrightsville Beach, North Carolina (NC). The analysis is based on production data from field trials and marketing data from the sale of tank-grown product. The growout facility consists of four 16.7-m3 (dia. x ht. = 5.58 × 1 m) fiberglass tanks supported by state-of-the-art RAS components, including particle traps and swirl separators, drum screen filter, trickling biological filter, UV sterilizer, heat pump, protein skimmer, and oxygen cone. Wild-caught, above minimum legal size black sea bass (24.2 cm TL, 350 g, 0.77 lb) were purchased from a commercial fisherman for $3.14/ kg ($1.4011b), stocked at a density of 21.1 kg/m3, and grown to a final weight of 1 kg (2.24 lb) in 200 d at 23 C resulting in 1.8 production cycles per year. Fish were fed a commercial pelleted diet ($0.94/kg; $0.42/Ib) with a feed conversion ratio of 1.5. Final harvest density was 60 kg/m3 (0.50 lb/gal), and total harvestable weight was 3,982 kg (8,919 Ibs) of fish per cycle, or 6,760 kg (15,022 lb) per year. The economic analysis assumes that the facility owner manages and operates the system on coastal property zoned commercial/industrial, where full strength seawater is available on demand from natural sources. Under the base case scenario, initial investment in construction and equipment is $84,506 (10-yr life), fish are grown to a harvestable weight of lkg/fish (2.24 lb/fish), product price (farm gate basis) is $10.10/kg ($4.50/lb), and breakeven price is $7.02/kg ($3.13/lb). Depreciation, fingerlings, interest paid, electricity, and feed, account for 19.6%,17.4%, 16.9%, 16.6%, and 12.3%, respectively, of total annual costs. Measures of financial performance for the base case, 10-yr scenario are: annual return to management, $18,819; net present value (5% discount rate), $145,313; internal rate of return on initial investment, 37%; and discounted payback period on initial investment, 3.2 yr. Sensitivity analysis showed that product price changes have the largest impact on annual returns, while changes in daily growth rate, initial weight, and survival have a strong impact on financial performance. Moderate effects are seen with changes in fingerling costs, feed costs, feed conversion ratio (FCR), final weight, and interest rates.
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  • 5
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Pilot-scale trials were conducted to evaluate growout performance of hatchery-reared summer flounder fingerlings in a state-of-the-art recirculating aquaculture system (RAS). The outdoor RAS consisted of four 4.57-m dia × 0.69-m deep (vol. =11.3 m3) covered, insulated tanks and associated water treatment components. Fingerlings (85.1 g mean initial weight) supplied by a commercial hatchery were stocked into two tanks at a density of 1,014 fish/tank (7.63 kg/m3). Fish were fed an extruded dry floating diet consisting of 50% protein and 12% lipid. The temperature was maintained between 20 C and 23 C and the salinity was 34 ppt. Under these conditions, growth, growth variation (CVwt), feed utilization, and survival of fish fed to 100% and 82% of a satiation rate were compared.Due to clear changes in growth patterns during the study, data was analyzed in three phases. During phase 1 (d 1–d 196), fish showed rapid growth, reaching a mean weight of 288 g ± 105 and 316 g ± 102, with a CVwt of 0.36 and 0.32 and FCR's of 1.38 and 1.36 in the subsatiation and satiation groups, respectively. During phase 2 (d 196–d 454), fish displayed slower growth reaching mean weights of 392 g ± 144 and 436 g ± 121, with a CVwt of 0.37 and 0.28, and increasing FCR's of 3.45 and 3.12 in the subsatiation and satiation groups, respectively. During phase 3 (d 454–d 614), fish showed little growth reaching mean weights of 399 g ± 153 and 440 g ± 129, with a CVwt of 0.38 and 0.29 in the subsatiation and satiation groups, respectively. Over the entire growout period (d 1–d 614), feed conversion ratios were 2.39 and 2.37 and survival was 75% and 81 % in the subsatiation and satiation treatments, respectively. The maximum biomass density reached during the study was 32.3 kg/m3.The satiation feed rate was superior to the 82% satiation rate, since it maximized growth rates, with no effect on FCR. The higher CVwt in the subsatiation group suggests increased competition for a restricted ration led to a slower growth with more growth variation. The decrease in growth in phases 2 and 3 was probably related to a high percentage of slower growing male fish in the population and the onset of sexual maturity.This study demonstrated that under commercial scale conditions, summer flounder can be successfully grown to a marketable size in a recirculating aquaculture system. Based on these results, it is recommended that a farmer feed at a satiation rate to minimize growout time. More research is needed to maintain high growth rates through marketable sizes through all-female production and/or inhibition of sexual maturity.
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  • 6
    Electronic Resource
    Electronic Resource
    Bingley : Emerald
    The @journal of product & brand management 14 (2005), S. 292-299 
    ISSN: 1061-0421
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Economics
    Notes: Purpose - This paper aims to explain the structure of the market from the perspective of small brands and to discuss marketing strategy implications. Design/methodology/approach - The paper uses revealed preference data of the Australian wine market, comprising 4,000 wine shoppers' purchases over a 12-month period. Standard brand performance measures such as penetration and purchase frequency are applied to the data to define niche and change-of-pace brands. Using the same data, price tier loyalty is measured using polarisation, and discussed in relation to the attribute offering required and the direct marketing approach required for true niche positions. Findings - The empirical results show that both niche and change-of-pace positions are prevalent in the wine market and small wineries, within a direct marketing channel approach, should target higher price points with branded wines but also lower price point products as well. The results suggest that attribute levels that are change-of-pace are unsustainable for small brands and can only be undertaken by large brands with the appropriate marketing resources. Research limitations/implications - The authors conceptualise that small brands should focus on attribute levels that have excess loyalty. Large brands can absorb attribute levels that are change-of-pace. This conceptualisation requires further discussion, particularly from the strategy literature, as well as further empirical testing. Practical implications - Whilst "niche" positions are the holy grail of some teaching and much practitioner endeavour, this paper has presented data that demonstrate the need for managers to ascertain if the position they occupy is in fact a niche or a change-of-pace position. Originality/value - This paper fulfils a need by using revealed preference behavioural data to highlight different strategies for small and large brands. Behavioural analysis and papers in the past have emphasised the strength and tendency towards large brands without offering insight into small brand strategies.
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  • 7
    Electronic Resource
    Electronic Resource
    Bingley : Emerald
    International journal of contemporary hospitality management 17 (2005), S. 553-568 
    ISSN: 0959-6119
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Economics
    Notes: Purpose - This paper analyzes the impact of menu descriptions on the selection of menu items. Furthermore, this paper examines the relationship between menu descriptions and the perceived value of the item. Design/methodology/approach - This study uses the different components of prospect theory (e.g. anchoring effects and framing effects). An experimental research design using mock menus was used to investigate the impact of item presentation, item selections, and menu descriptions on consumer judgments of consumer choice and price value. Findings - The results found that detailed menu descriptions negated the impact of the price increases on the menu items. Practical implications - The implications of this study are valuable to restaurateurs because it shows that menu descriptions have the potential to increase revenue while also increasing the value perception. The study can also be applied to similar competing restaurants. Restaurants can be successful when magnifying the differences with detailed descriptions. Originality/value - The implications of this study can aid restaurateurs that are either developing new menus or increasing their prices. Restaurateurs are encouraged to provide a more detailed menu description in order to increase the perceived value by the guest.
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  • 8
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature genetics 37 (2005), S. 212-213 
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] In eukaryotes, the most abundant covalent modification of DNA is methylation of cytosine residues at carbon 5 of the pyrimidine ring. This modification occurs primarily in the context of a simple sequence (5′-CG-3′) and affects both strands of DNA. CpG methylation serves to increase the ...
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  • 9
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature genetics 37 (2005), S. 1175-1180 
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Characterizing the patterns of genetic variation in an organism provides fundamental insight into the evolutionary history of the organism and defines the scope and nature of studies that must be designed to correlate genotype to phenotype. Given the pre-eminent role of the inbred mouse in ...
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  • 10
    Electronic Resource
    Electronic Resource
    [s.l.] : Macmillian Magazines Ltd.
    Nature 435 (2005), S. 479-482 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The marine sedimentary record exhibits evidence for episodes of enhanced organic carbon burial known as ‘oceanic anoxic events’ (OAEs). They are characterized by carbon-isotope excursions in marine and terrestrial reservoirs and mass extinction of marine faunas. Causal mechanisms ...
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