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  • 11
    Publication Date: 2018-08-09
    Description: Several methanogenic pathways in oxic surface waters were recently discovered, but their relevance in the natural environment is still unknown. Our study examines distinct methane enrichments that repeatedly occur below the thermocline during the summer months in the central Baltic Sea. In agreement with previous studies in this region, we discovered differences in the methane distributions between the Western and Eastern Gotland Basin, pointing to in situ methane production below the thermocline in the latter (conc. CH4 14.1±6.1nM, δ13C CH4 −62.9‰). Through the use of a high resolution hydrographic model of the Baltic Sea, we showed that methane below the thermocline can be transported by upwelling events towards the sea surface thus contributing to the methane flux at the sea/air interface. To quantify zooplankton-associated methane production rates, we developed a sea-going methane stripping-oxidation line to determine methane release rates from copepods grazing on 14C-labelled phytoplankton. We found that: (1) methane production increased with the number of copepods, (2) higher methane production rates were measured in incubations with Temora longicornis (125±49 fmol methane copepod−1d−1) than incubations with Acartia spp. (84±19 fmol CH4 copepod−1d−1) dominated zooplankton communities, and (3) methane was only produced on a Rhodomonas sp. diet, but not on a cyanobacteria diet. Furthermore, copepod-specific methane production rates increased with incubation time. The latter finding suggests that methanogenic substrates for water-dwelling microbes are released by cell disruption during feeding, defecation, or diffusion from fecal pellets. In the field, particularly high methane concentrations coincided with stations showing a high abundance of DMSP-rich Dinophyceae. Lipid biomarkers extracted from phytoplankton- and copepod-rich samples revealed that Dinophyceae are a major food source of the T. longicornis dominated zooplankton community, supporting the proposed link between copepod grazing, DMSP release, and the buildup of subthermocline methane enrichments in the central Baltic Sea.
    Print ISSN: 1810-6277
    Electronic ISSN: 1810-6285
    Topics: Biology , Geosciences
    Published by Copernicus on behalf of European Geosciences Union.
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  • 12
  • 13
    Publication Date: 2018-08-29
    Description: Marine microorganisms trapped in amber are extremely rare in the fossil record, and the few existing inclusions recovered so far originate from very few pieces of Cretaceous amber from France. Marine macroscopic inclusions are also very rare and were recently described from Cretaceous Burmese amber and Early Miocene Mexican amber. Whereas a coastal setting for the amber source forests is generally proposed, different scenarios have been suggested to explain how these marine inclusions can become trapped in a resin of terrestrial origin. These scenarios include an introduction of marine organisms (i) through high tides, (ii) from storms and resulting in flooding of the littoral/estuarine forest floor, (iii) in resin dropped into the sea in mangrove-type settings, or (iv) by wind and sea spray. We investigated the possibility of a wind-driven introduction of marine microorganisms into tree resins using modern coastal conifer forests with the highly resinous Cook pine (Araucaria columnaris) in New Caledonia as a model for the Cretaceous amber forests from France. By exposing fresh resin surfaces on the seaward side of the trees and the collection of older in situ resins, we confirmed that marine microorganisms can become trapped on sea-exposed resin, along with remnants from terrestrial organisms, and salt crystals. We suggest that, for cases where only a few marine inclusions are discovered in an amber deposit, an origin from aeolian background deposition is feasible. However, a more energetic but possibly still aeolian event is likely needed to explain the high numbers of marine microorganisms embedded in pieces of Cretaceous amber from France.
    Print ISSN: 2193-0066
    Electronic ISSN: 2193-0074
    Topics: Geosciences
    Published by Copernicus
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  • 14
    Publication Date: 2017-06-28
    Description: High concentrations of free C32 bis-homohopanoic acids (up to 433 μg/g dry wt) occur in microbial mats at methane seeps in anoxic Black Sea waters. These compounds show a strong preference for the ‘geological’ 17α(H),21β(H)- over the ‘biological’ 17β(H),21β(H)-configuration (αβ/ββ ratios up to 30.7) and indicate the potential formation of αβ-hopanoids in modern environments. Strong 13C-depletions (δ13C as low as −78.4‰ PDB) indicate an in situ generation of these hopanoids by biota involved in the anaerobic cycling of methane carbon. The inferred presence of hopanoids indigenous to a permanently anoxic marine environment is significant because these lipids are not known to occur in strictly anaerobic bacteria.
    Type: Article , PeerReviewed
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  • 15
    Publication Date: 2021-03-18
    Description: Magmatic sill intrusions into organic-rich sediments cause the release of thermogenic CH4 and CO2. Pore fluids from the Guaymas Basin (Gulf of California), a sedimentary basin with recent magmatic activity, were investigated to constrain the link between sill intrusions and fluid seepage as well as the timing of sill-induced hydrothermal activity. Sampling sites were close to a hydrothermal vent field at the northern rift axis and at cold seeps located up to 30km away from the rift. Pore fluids close to the active hydrothermal vent field showed a slight imprint by hydrothermal fluids and indicated a shallow circulation system transporting seawater to the hydrothermal catchment area. Geochemical data of pore fluids at cold seeps showed a mainly ambient diagenetic fluid composition without any imprint related to high temperature processes at greater depth. Seep communities at the seafloor were mainly sustained by microbial methane, which rose along pathways formed earlier by hydrothermal activity, driving the anaerobic oxidation of methane (AOM) and the formation of authigenic carbonates. Overall, our data from the cold seep sites suggest that at present, sill-induced hydrothermalism is not active away from the ridge axis, and the vigorous venting of hydrothermal fluids is restricted to the ridge axis. Using the sediment thickness above extinct conduits and carbonate dating, we calculated that deep fluid and thermogenic gas flow ceased 28 to 7kyr ago. These findings imply a short lifetime of hydrothermal systems, limiting the time of unhindered carbon release as suggested in previous modeling studies. Consequently, activation and deactivation mechanisms of these systems need to be better constrained for the use in climate modeling approaches.
    Type: Article , PeerReviewed
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  • 16
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    Springer Berlin Heidelberg | Berlin/Heidelberg
    Publication Date: 2021-03-29
    Description: Characeae, a family of calcifying green algae, are common in carbonate-rich freshwaters. The southwestern shoreline of Lake Ganau (Kurdistan Region, northeastern Iraq) harbors dense and thick mats of these algae (genus Chara). On the lake bottom and along the shore, carbonate sands and rocks rich in the remains of stems, branches, nodes, and whorls of Chara are deposited. These deposits show all stages of growth and degradation of characean algae, including replacement and lithification into limestone. The replacement of the fragments by fine-grained calcite preserved delicate microstructures of Chara, such as cortical walls, cell shape, inner and outer layers of the stems, and reproductive organs. Based on roundness, sorting, the degree of lithification, and preserved microstructures of the grains (fragments), three facies were recognized. The first is represented by a newly formed lime sand facies showing elongated grains, poor sorting, and reduced roundness, with pristine preservation of characean surface microstructures. The second is a weathered lime sand facies, which shows better sorting and good roundness, whereas internal structures of characean fragments are still well preserved. The third is comprised of a lithified lime sand facies (grainstone), with very well sorted and rounded grains, and poorly preserved external and internal structures of the characeans. As compared to the newly formed lime sand facies, the grainstone facies shows an increase in grain size by more than 30 %, owing to precipitation of micritic lamina of possible microbial origin. Eventually, the Characeae-derived lime sands are lithified into oolitic limestones with sparry calcite cement, forming a grainstone microfacies. The present study has important implications for the interpretation of pre-Quaternary environments, as it records all stages of the fossilization process of characean green algae and highlights the role of these algae in the formation of oolitic carbonate rocks.
    Keywords: Characeae; Chara; Ooids; Green algae; Carbonate sand; Freshwater carbonates; Facies ; 551 ; Earth Sciences; Sedimentology; Biogeosciences; Geochemistry; Paleontology; Ecology
    Language: English
    Type: article , publishedVersion
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  • 17
    Publication Date: 2021-03-29
    Description: Microbial iron oxyhydroxides are common deposits in natural waters, recent sediments, and mine drainage systems. Along with these minerals, trace and rare earth elements (TREE) are being accumulated within the mineralizing microbial mats. TREE patterns are widely used to characterize minerals and rocks, and to elucidate their evolution and origin. However, whether and which characteristic TREE signatures distinguish between a biological and an abiological origin of iron minerals is still not well-understood. Here we report on long-term flow reactor studies performed in the Tunnel of Äspö (Äspö Hard Rock Laboratory, Sweden). The development of microbial mats dominated by iron-oxidizing bacteria (FeOB), namely Mariprofundus sp. and Gallionella sp were investigated. The feeder fluids of the flow reactors were tapped at 183 and 290 m below sea-level from two brackish, but chemically different aquifers within the surrounding, ~1.8 Ga old, granodioritic rocks. The experiments investigated the accumulation and fractionation of TREE under controlled conditions of the subsurface continental biosphere, and enabled us to assess potential biosignatures evolving within the microbial iron oxyhydroxides. After 2 and 9 months, concentrations of Be, Y, Zn, Zr, Hf, W, Th, Pb, and U in the microbial mats were 103- to 105-fold higher than in the feeder fluids whereas the rare earth elements and Y (REE+Y) contents were 104- and 106-fold enriched. Except for a hydrothermally induced Eu anomaly, the normalized REE+Y patterns of the microbial iron oxyhydroxides were very similar to published REE+Y distributions of Archaean Banded Iron Formations (BIFs). The microbial iron oxyhydroxides from the flow reactors were compared to iron oxyhydroxides that were artificially precipitated from the same feeder fluid. Remarkably, these abiotic and inorganic iron oxyhydroxides show the same REE+Y distribution patterns. Our results indicate that the REE+Y mirror closely the water chemistry, but they do not allow to distinguish microbially mediated from inorganic iron precipitates. Likewise, all TREE studied showed an overall similar fractionation behavior in biogenic, abiotic, and inorganic iron oxyhydroxides. Exceptions are Ni and Tl, which were only accumulated in the microbial iron oxyhydroxides and may point to a potential utility of these elements as microbial biosignatures.
    Keywords: biosignatures; microbialmats; microbialironoxides; traceelements; rareearthelements; microbe–metalinteraction; bandedironformation ; 551
    Language: English
    Type: article , publishedVersion
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  • 18
    Publication Date: 2021-03-29
    Description: We propose that organic compounds found in a Miocene limestone from Marmorito (Northem Italy) are source markers for organic matter present in aneient methane vent systems (cold seeps). The limestone contains high concentrations of the tail-to-taillinked, acyclic C20 isoprenoid 2,6,11,15-tetramethylhexadecane (crocetane), a C25 homolog 2,6,10,15,19-pentamethylicosane (PME), and a distinctive glycerol ether lipid containing 3,7,1l,15-tetramethylhexadecyl (phytanyl-) moieties. The chemical structures of these biomarkers indicate a common origin from archaea. Their extreme1y l3C-depleted isotope compositions (813C "'" -108 to -115.6%0 PDB) suggest that the respective archaea have directly or indirectly introduced isotopically depleted, methane-derived carbon into their biomass. We postulate that a second major cluster of biomarkers showing heavier isotope values (8l3C "'" -88%0) is derived from sulfate-redueing bacteria (SRB). The observed biomarkers sustain the idea that methanogenic bacteria, in a syntrophic community with SRB, are responsible for the anaerobic oxidation of methane in marine sediments. Marmorito may thus represent a conceivable aneient scenario for methane consumption performed by a defined, two-membered bacterial consortium: (1) archaea that perform reversed methanogenesis by oxidizing methane and produeing CO2 and H2; and (2) SRB that consume the resulting H2. Furthermore, the respective organic molecules are, unlike other compounds, tightly bound to the crystalline carbonate phase. The Marmorito carbonates can thus be regarded as "cold seep microbialites" rather than mere "authigenic" carbonates.
    Keywords: Paläontologie: Allgemeines ; 551 ; VU 000 ; 38.20 ; 38.2
    Language: English
    Type: article , publishedVersion
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  • 19
    Publication Date: 2021-04-25
    Description: The lipid assemblages of the "living fossil" stromatoporoid Astrosclera willeyana (Great Barrier Reet) and the demosponge Agelas aroides (Mediterranean Sea) were investigated. Large amounts of branched carboxylic acids are present in the sponges studied. These compounds include terminally branched carboxylic acids (isa -/anteisa-) and abundant mid-chain branched carboxylic acids (MBCA) wh ich are characterized by an intriguing variety of structural isomers present in the C 15- C25 range. The most prominent MBCA are comprised of isomeric methylhexadecanoic acids and methyloctadecanoic acids. A second cluster of MBCA includes methyldocosanoic acids and methyltetracosanoic acids, but other homologues are also present. Methyl branching points were generally observed between the w5- and w9-positions. These complex isomeric mixtures apparently derive from symbiotic bacteria living exclusively in demosponges. Comparison with hydrocarbon compositions of ancient carbonates reveals evidence that the MBCA found are potential lipid precursors of mid-chain branched monomethylalkanes often observed in fossil sediments and oils. As a working hypo thesis, we suggest that their bacterial source organisms have been widespread in the geological past, and are found " inherited" in the protective environment of distinctive sponge hosts in recent marine ecosystems. Furthermore, both sponges contain abundant linear, longchain C24- C26 dienoic "demospongic" acids. The demospongic acid distribution and the presence of phytanic acid in A. willeyana match the patterns found in A. aroides and other members of the Agelasida. These findings confirm the systematic position of A. willeyana within this demosponge taxon.
    Keywords: Paläontologie: Allgemeines ; 551 ; VU 000 ; 38.20 ; 38.2
    Language: English
    Type: article , publishedVersion
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  • 20
    Publication Date: 2021-03-29
    Description: Paleoarchean rocks from the Pilbara Craton of Western Australia provide a variety of clues to the existence of early life on Earth, such as stromatolites, putative microfossils and geochemical signatures of microbial activity. However, some of these features have also been explained by non-biological processes. Further lines of evidence are therefore required to convincingly argue for the presence of microbial life. Here we describe a new type of microbial mat facies from the 3.4 Ga Strelley Pool Formation, which directly overlies well known stromatolitic carbonates from the same formation. This microbial mat facies consists of laminated, very fine-grained black cherts with discontinuous white quartz layers and lenses, and contains small domical stromatolites and wind-blown crescentic ripples. Light- and cathodoluminescence microscopy, Raman spectroscopy, and time of flight-secondary ion mass spectrometry (ToF-SIMS) reveal a spatial association of carbonates, organic material, and highly abundant framboidal pyrite within the black cherts. Nano secondary ion mass spectrometry (NanoSIMS) confirmed the presence of distinct spheroidal carbonate bodies up to several tens of μm that are surrounded by organic material and pyrite. These aggregates are interpreted as biogenic. Comparison with Phanerozoic analogues indicates that the facies represents microbial mats formed in a shallow marine environment. Carbonate precipitation and silicification by hydrothermal fluids occurred during sedimentation and earliest diagenesis. The deciphered environment, as well as the δ13C signature of bulk organic matter (-35.3‰), are in accord with the presence of photoautotrophs. At the same time, highly abundant framboidal pyrite exhibits a sulfur isotopic signature (δ34S = +3.05‰; Δ33S = 0.268‰; and Δ36S = -0.282‰) that is consistent with microbial sulfate reduction. Taken together, our results strongly support a microbial mat origin of the black chert facies, thus providing another line of evidence for life in the 3.4 Ga Strelley Pool Formation.
    Description: Open-Access Publikationsfonds 2016
    Keywords: Carbonates; Sulfur; Isotopes; Organic materials; Crystals; Paleoclimatology; Paleoenvironments; Quartz ; 551
    Language: English , English
    Type: article , publishedVersion
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