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  • 1
    Electronic Resource
    Electronic Resource
    Application of Conductimetry for Evaluation of Lactic Starter Cultures in Soymilk (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 67 (2002), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : :Soymilk (SM), a potential culture medium for applying conductimetric techniques to evaluate the behavior of probiotic lactobacilli and bifidobacteria was examined. Media LAPTg, LM, and SM was standardized for the growth of Lactobacillus fermentum and Bifidobacterium longum. A dilution of 1/100 for L. fermentum and 1/1 for B. longum were considered optimal to obtain a detection time (DT) between 5 and 10 min. The relationship between the initial number of cells and the DT was linear throughout the incubation period considered (40 h). The effect of temperature (30, 37, and 42 °C) on the metabolic activity of the cells was also determined after 40 h of fermentation. The higher metabolic changes were observed at 42 °C with conductance values from 600 to 800 μS and DT of 5-7 min. Results obtained were confirmed by viable counts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2002.tb09472.x
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  • 2
    Electronic Resource
    Electronic Resource
    Competitive hybridization on spotted microarrays as a tool to conduct comparative genomic analyses of Xylella fastidiosa strains (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 216 (2002), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Xylella fastidiosa strains are responsible for several plant diseases and since such isolates display a broad host range and complex biological behavior, genomic comparisons employing microarray hybridizations may provide an effective method to compare them. Thus, we performed a thorough validation of this type of approach using two recently sequenced strains of this phytopathogen. By matching microarray hybridization results to direct sequence comparisons, we were able to establish precise cutoff ratios for common and exclusive sequences, allowing the identification of exclusive genes involved in important biological traits. This validation will enable the use of microarray-based comparisons across a wide variety of microorganisms
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2002.tb11408.x
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  • 3
    Electronic Resource
    Electronic Resource
    Ban AI a new isoschizomer of the type II restriction endonuclease Hae III discovered in a Bacillus anthracis isolate from Amazon Basin (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 215 (2002), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Bacillus anthracis was isolated and identified from a bacterial collection of samples from the Amazon river bank. Type II restriction endonuclease activity was detected in this prokaryote, the enzyme was purified, the molecular mass of the native protein estimated by gel filtration, and optima pH, temperature and salt requirements were determined. Quality control assays showed complete absence of ‘non-specific nucleases’. Restriction cleavage analysis and DNA sequencing of restriction fragments allowed unequivocal demonstration of 5′-GG↓CC-3′ as the recognition sequence. This enzyme was named Ban AI and is therefore an isoschizomer of the prototype restriction endonuclease Hae III. This is the first report of a type II restriction endonuclease identified, purified from a natural isolate of B. anthracis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2002.tb11376.x
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  • 4
    Electronic Resource
    Electronic Resource
    Evaluation of aluminium tolerance in a spring rye collection by hydroponic screening (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 121 (2002), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Aluminium (A1) tolerance of 63 rye accessions from a world spring rye collection was evaluated using the haematoxylin method and the root growth method. The haematoxylin method is based on the ability of A1-tolerant seedlings to continue root growth following a short pulse treatment with a high Al concentration, while the root growth method uses the root growth and root tolerance index to judge A1 tolerance. Significantly higher levels of A1 tolerance were found in rye than in the A1-tolerant bread wheat cultivar ‘Maringa’. Under the assumption that the ability of roots to grow under A1 stress is a combination of root vigour (long roots) and A1 tolerance, a hypothesis allowing for the differentiation of five genotype classes was suggested. This study demonstrated that the haematoxylin method and the root growth parameter identify genotypes with long root growth under A1 stress, but failed to detect A1 tolerance in genotypes with poor root vigour. These genotypes can only be identified using the root tolerance index parameter. However, the haematoxylin method is highly suitable for screening large segregating populations derived from improved germplasm that has been preselected for agronomically preferable traits, including plant vigour.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2002.00706.x
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  • 5
    Electronic Resource
    Electronic Resource
    Inheritance of reduced linolenic acid content in the Ethiopian mustard mutant N2-4961 (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 121 (2002), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The zero erucic acid Ethiopian mustard lines developed so far are characterized by an exceptionally high linolenic acid content in the seed oil. The mutant line N2-4961, expressing low linolenic acid content in a high erucic acid background, was developed through chemical mutagenesis. The objective of this research was to study the inheritance of low linolenic acid content in this mutant. Line N2-4961 was reciprocally crossed with its parent line C-101 and the linolenic acid content of the reciprocal F1, F2 and BC1 generations was studied. No maternal, cytoplasmic or dominance effects were detected in the analysis of F1 seeds and F1 plants from reciprocal crosses. Linolenic acid content segregated in 1: 2: 1 ratios in all the F2 populations studied, suggesting monogenic inheritance. This was confirmed with the analysis of the reciprocal backcross generation. The simple inheritance of low linolenic acid content in N2-4961 will facilitate the transference of this trait to zero erucic acid lines of Ethiopian mustard.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2002.00714.x
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  • 6
    Electronic Resource
    Electronic Resource
    Traits of brown trout prey in relation to habitat characteristics and benthic invertebrate communities (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 60 (2002), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A trait database based on habitat, behavioural and morphological characteristics of invertebrates enabled invertebrates known to have differential vulnerability to salmonid predation to be discriminated. Among all of these traits the most important were associated with invertebrate spatial proximity to salmonids (macrohabitat preference), potential conspicuousness and accessibility (e.g. flow exposure, mobility, tendency to drift, trajectory and agility) and handling efficiency (body flexibility and size). Data on benthos assemblages and brown trout salmo trutta diets collected over two consecutive summers in different macrohabitat types (e.g. pools, runs and riffles) of two Pyrenean streams demonstrated that: (i) decrease in torrential flow characteristics of habitats was associated with changes in invertebrate body shape and movement, and a decrease in body flexibility and tendency to drift; (ii) brown trout tended to feed on the most vulnerable invertebrates (e.g. those living in exposed microhabitats and with a high tendency to drift, agility and aggregation), except in faster flowing habitats. The results suggest that the potential vulnerability of benthic invertebrates increased with increases in flow (torrential) characteristics. Also, the database compiled on traits explained differences among brown trout diets, especially in slow flowing areas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.2002.tb01694.x
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  • 7
    Electronic Resource
    Electronic Resource
    First record of the Asiatic cyprinid Pseudorasbora parva in the Iberian Peninsula (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 61 (2002), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Pseudorasbora parva is recorded for the first time in the Iberian Peninsula. Its presence in the River Ebro delta is probably due to involuntary introduction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.2002.tb01864.x
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  • 8
    Electronic Resource
    Electronic Resource
    Some morphometric and biochemical features of ready-to-migrate silver and pre-migratory yellow stages of the shortfin eel of south-eastern Australian waters (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 61 (2002), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The mean total length (LT), mass and age of ready to migrate female silver shortfin eels Anguilla australis from the Hopkins River estuary and the mouth of the Merri River in south-eastern Australia, were 83·2 ± 1·2 cm, 1051 ± 51 g, and 17·2 ± 1·79 years, respectively. The eye index (IE) of the silver shortfin eels was 〈 5·2 (mean 7·64 ± 0·29) and differed significantly from that of the yellow shortfin eels collected from two other sites. The IE increased with LT (mm) and was related by log IE= 2·656 log LT6·925. The per cent moisture, protein and ash content of the liver of silver shortfin eels was significantly lower than in yellow shortfin eels, but lipid content was significantly higher in the former (35·5 ± 2·0%). The mean mass μg mg lipid −) of saturates (230·4 ± 2·6 v. 181·7 ±2·6), monoenes (367·4 ± 6·3 v. 290·8 ± 8·9) and PUFA (177·3 ± 5·3 v. 159·7 ± 4·6) in muscle was significantly higher, and the great majority of individual fatty acids was found also in higher quantities in silver shortfin eels. In the liver, the PUFA found in the highest quantity was 22:6n-3, except in shortfin eels from Hopkins River estuary, and the amount of 18:2n-6 in the liver of silver shortfin eels was significantly higher than that in yellow shortfin eels but the reverse was true of 20:4n-6. In both muscle and liver tissues the saturate 16:0 and the monoene 18:ln-9 collectively accounted for 〉50% of all the fatty acids in the lipid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.2002.tb01852.x
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  • 9
    Electronic Resource
    Electronic Resource
    Effect of Cooking on the Crispness of Cassava Chips (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 67 (2002), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : :Technology for the production of edible cassava chips was studied, using pretreatments such as cooking and partial drying to improve chip crispness. Different cassava varieties and types of cooking, such as boiling water and steam, were also investigated. The effects of such treatments were evaluated on chip friability criteria by using the Texture Analyzer TA.XT2, as well as affective tests for sensorial measurement. A significant correlation (-0.90592; p≤ 0.05) was found between friability and sensory acceptance of chips from the cassava variety “IAC Mantiqueira.” The absence of correlation (-0.66069; p 〈 0.05) for the variety “IAC 576.70” was considered highly positive, since the chips were sensorially accepted despite their high friability values.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2002.tb09480.x
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  • 10
    Electronic Resource
    Electronic Resource
    Nucleotide structure of the Scytalidium hyalinum and Scytalidium dimidiatum 18S subunit ribosomal RNA gene: evidence for the insertion of a group IE intron in the rDNA gene of S. dimidiatum (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 208 (2002), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The molds Scytalidium dimidiatum (Nattrassia mangiferae synanamorph) and Scytalidium hyalinum are responsible for dermatomycosis in humans. We sequenced their 18S subunit ribosomal RNA gene to identify these species with molecular biology-based methods. The coding sequences differed by a single polymorphism (A in S. dimidiatum, G in S. hyalinum). Moreover, we found an insert at position 1199 in the 18S rRNA gene sequence of S. dimidiatum. Its potential secondary structure was characteristic of a group IE intron. Bioinformatic and phylogenic group IE intron analyses generated four main homogeneous clusters. The S. dimidiatum intron is original and not related with other known IE group introns.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2002.tb11080.x
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