Publication Date:
2002-03-09
Description:
Time courses of translocation of fluorescently conjugated proteins to the plasma membrane were simultaneously measured in thousands of individual rat basophilic leukemia cells. We found that the C2 domain---a calcium-sensing, lipid-binding protein module that is an essential regulator of protein kinase C and numerous other proteins---targeted proteins to the plasma membrane transiently if calcium was released from internal stores, and persistently in response to entry of extracellular calcium across the plasma membrane. The C2 domain translocation time courses of stimulated cells clustered into only two primary modes. Hence, the reversible recruitment of families of signaling proteins from one cellular compartment to another is a rapid bifurcation mechanism for inducing discrete states of cellular signaling networks.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Teruel, Mary N -- Meyer, Tobias -- CA83229/CA/NCI NIH HHS/ -- GM062144/GM/NIGMS NIH HHS/ -- HG00057/HG/NHGRI NIH HHS/ -- New York, N.Y. -- Science. 2002 Mar 8;295(5561):1910-2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Pharmacology, Stanford University Medical School, 269 Campus Drive, Stanford, CA 94305, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11884760" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Bacterial Proteins
;
Calcium/*metabolism
;
*Calcium Signaling
;
Cell Membrane/*metabolism
;
Cytosol/metabolism
;
Fluorescence
;
Fluorescent Dyes
;
Isoenzymes/chemistry/*metabolism
;
Kinetics
;
Luminescent Proteins
;
Platelet Activating Factor/pharmacology
;
Protein Binding
;
Protein Kinase C/chemistry/*metabolism
;
Protein Structure, Tertiary
;
*Protein Transport
;
Rats
;
Receptors, Cell Surface/*metabolism
;
Recombinant Fusion Proteins/metabolism
;
Software
;
Thapsigargin/pharmacology
;
Transfection
;
Tumor Cells, Cultured
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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