Publication Date:
2002-04-06
Description:
The essential Cdc13 protein in the yeast Saccharomyces cerevisiae is a single-stranded telomeric DNA binding protein required for chromosome end protection and telomere replication. Here we report the solution structure of the Cdc13 DNA binding domain in complex with telomeric DNA. The structure reveals the use of a single OB (oligonucleotide/oligosaccharide binding) fold augmented by an unusually large loop for DNA recognition. This OB fold is structurally similar to OB folds found in the ciliated protozoan telomere end-binding protein, although no sequence similarity is apparent between them. The common usage of an OB fold for telomeric DNA interaction demonstrates conservation of end-protection mechanisms among eukaryotes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mitton-Fry, Rachel M -- Anderson, Emily M -- Hughes, Timothy R -- Lundblad, Victoria -- Wuttke, Deborah S -- GM55867/GM/NIGMS NIH HHS/ -- GM59414/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2002 Apr 5;296(5565):145-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11935027" target="_blank"〉PubMed〈/a〉
Keywords:
Binding Sites
;
DNA, Fungal/chemistry/*metabolism
;
DNA, Single-Stranded/chemistry/*metabolism
;
DNA-Binding Proteins/*chemistry/metabolism
;
Ligands
;
Models, Molecular
;
Nuclear Magnetic Resonance, Biomolecular
;
Protein Binding
;
Protein Conformation
;
Protein Folding
;
Protein Structure, Secondary
;
Protein Structure, Tertiary
;
Saccharomyces cerevisiae Proteins/*chemistry/metabolism
;
Telomere/*metabolism
;
*Telomere-Binding Proteins
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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