Publikationsdatum:
2001-08-18
Beschreibung:
The homodimeric nickel-containing CO dehydrogenase from the anaerobic bacterium Carboxydothermus hydrogenoformans catalyzes the oxidation of CO to CO2. A crystal structure of the reduced enzyme has been solved at 1.6 angstrom resolution. This structure represents the prototype for Ni-containing CO dehydrogenases from anaerobic bacteria and archaea. It contains five metal clusters of which clusters B, B', and a subunit-bridging, surface-exposed cluster D are cubane-type [4Fe-4S] clusters. The active-site clusters C and C' are novel, asymmetric [Ni-4Fe-5S] clusters. Their integral Ni ion, which is the likely site of CO oxidation, is coordinated by four sulfur ligands with square planar geometry.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dobbek, H -- Svetlitchnyi, V -- Gremer, L -- Huber, R -- Meyer, O -- New York, N.Y. -- Science. 2001 Aug 17;293(5533):1281-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Max-Planck-Institut fur Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, D-82152 Martinsried, Germany. dobbek@biochem.mpg.de〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11509720" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Aldehyde Oxidoreductases/*chemistry/*metabolism
;
Bacteria, Anaerobic/*enzymology
;
Binding Sites
;
Carbon Dioxide/metabolism
;
Carbon Monoxide/*metabolism
;
Catalysis
;
Chemistry, Physical
;
Crystallization
;
Crystallography, X-Ray
;
Dimerization
;
Electron Transport
;
Hydrogen Bonding
;
Iron/*chemistry/metabolism
;
Ligands
;
Models, Molecular
;
Multienzyme Complexes/*chemistry/*metabolism
;
Nickel/*chemistry/metabolism
;
Oxidation-Reduction
;
Peptococcaceae/*enzymology
;
Physicochemical Phenomena
;
Protein Conformation
;
Protein Structure, Secondary
;
Protein Structure, Tertiary
;
Protein Subunits
;
Sulfur/*chemistry/metabolism
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
Permalink
