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  • Transformation  (4)
  • FPGA  (3)
  • Springer  (7)
  • American Society of Hematology
  • Blackwell Publishing Ltd
  • Oxford University Press
  • 2000-2004  (7)
  • 2000  (7)
Collection
Publisher
  • Springer  (7)
  • American Society of Hematology
  • Blackwell Publishing Ltd
  • Oxford University Press
Years
  • 2000-2004  (7)
Year
  • 2000  (7)
  • 1
    ISSN: 1432-203X
    Keywords: Key words Agrobacterium rhizogenes ; Crotalaria juncea ; Plant regeneration ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Hairy roots were induced from leaf segments of Crotalaria juncea, which is used as a green manure crop antagonistic to nematodes, by infection with a mikimopine type wild strain of Agrobacterium rhizogenes A13 (MAFF02-10266). These roots exhibited vigorous growth and abundant lateral branching on half-strength Murashige and Skoog (1/2MS) medium without phytohormones. The adventitious shoots were induced from 30% of root segments 3 months after transfer onto medium containing 3 mg/l benzyl adenine. These shoots produced roots 1 month after transfer onto 1.2% agar-solidified 1/2MS medium without phytohormones. Regenerated plants were successfully grown under greenhouse conditions. The transgenic nature of the regenerated plants was confirmed by Southern-blot analysis.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0983
    Keywords: Key words Basidiomycete ; Transformation ; White-rot fungi ; Carboxin ; Succinate:ubiquinone oxidoreductase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel selection marker gene for transformation of the white-rot basidiomycete Pleurotus ostreatus was developed by introducing a point mutation in a gene which encodes the iron-sulfur protein (Ip) subunit of succinate dehydrogenase. The mutant gene, Cbx R, encodes a modified Ip subunit with an amino-acid substitution (His239 to Leu) and confers resistance to the systemic fungicide, carboxin. The DNA sequence was integrated ectopically in the chromosome of the transformants. This is the first report of a homologous marker gene which is available for the molecular breeding of an edible mushroom.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 19 (2000), S. 1184-1187 
    ISSN: 1432-203X
    Keywords: Keywords Zygote ; In vitro culture ; Electroporation ; Transformation ; Tobacco ; AbbreviationsNAA: Naphthaleneacetic acid ; ¶BA: 6-Benzyladenine ; GUS: β-Glucuronidase ; ¶GFP: Green fluorescent protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have established a technique for isolating, culturing and transforming tobacco zygotes. Zygotes were isolated by microdissection or enzymatic maceration from fertilized embryo sacs. Viable zygotes cocultured with mesophyll protoplasts underwent first division after 3 days of culture. Zygotes isolated by microdissection underwent a higher frequency of first division (61.2%) than those isolated by enzymatic maceration (30.5%). Globular embryos were formed only from microdissected zygotes, at a frequency of 8.7% after 1–2 weeks in culture. An efficient millicell device for the electroporation of DNA into zygotes was established. The electroporated zygotes divided in vitro at a frequency of 54.6% and developed into proembryos. Introduced GFP gene constructs showed transient expression in about 2.6% of the electroporated tobacco zygotes.
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  • 4
    ISSN: 1432-203X
    Keywords: Key words Gentian ; Hygromycin phosphotransferase gene (hpt) ; Particle bombardment ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Cell suspension cultures were established from leaf explants of gentian (Gentiana triflora×G. scabra) for the generation of transgenic plants by particle bombardment. The parameters for the bombardment of suspension culture cells with a particle gun were examined by monitoring the transient expression of a gene for β-glucuronidase driven by the cauliflower mosaic virus (CaMV) 35S promoter. We found that prior culture of suspension culture cells for 5 days on solid medium was optimum for successful particle bombardment. Putative transformed calli were obtained from bombarded cells after a two-step selection procedure. Cells were cultured first with 30 mg l–1 hygromycin in liquid MS medium that contained 10 mg l–1 N-phenyl-N′-1,2,3-thiadiazol-5-yl urea, 1 mg/l 1-naphthaleneacetic acid and 30 g l–1 sucrose and then on solid medium prepared from the same liquid medium plus 2 g l–1 gellan gum. After 12 weeks of selection on solid medium that contained 30 mg l–1 hygromycin, two transgenic gentian plants were regenerated from each selected callus. Analysis by the polymerase chain reaction and Southern blotting revealed the stable integration of transferred DNA.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of electronic testing 16 (2000), S. 289-299 
    ISSN: 1573-0727
    Keywords: FPGA ; test ; ATPG ; iterative testing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract This paper describes an approach to minimize the number of test configurations for testing the logic cells of a RAM-based FPGA taking into account the configurability of such flexible device. The proposed approach concerns the XILINX 4000 family. On this example of FPGA, a bottom-up test technique is first used to generate test configurations for the elementary modules, then test configurations for a single logic cell, and finally test configurations for the m × m array of logic cells. In this bottom-up technique, it is shown that the key point is the minimization of the number of test configurations for a logic cell. An approach for the logic cell of the XILINX4000 family is then described to define a minimum number of test configurations knowing the test configurations of its logic modules. This approach gives only 5 test configurations for the XILINX4000 family while the previous published works concerning Boolean testing of this FPGA family gives 8 or 21 test configurations.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of electronic testing 16 (2000), S. 513-520 
    ISSN: 1573-0727
    Keywords: VLSI ; FPGA ; test ; ATPG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract This paper addresses the problem of testing the configurable modules used in the local interconnect of SRAM-based FPGAs. First, it is demonstrated that a n address bit Configurable Interface Multiplexer requires N = 2 n test configurations considering a stuck-at as well as a functional fault model. Second, a logic cell with a set of k input Configurable Interface Modules with n address bits is analyzed and it is proven that the set of CIMs can be tested in parallel making the number of required test configurations equal to N = 2 n . Third, it is shown that the complete circuit i.e. a m × m array of sets of k Configurable Interface Multiplexers with n address bits can be tested with only N = 2 n test configurations using the XOR tree and shift register structures.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Real-time systems 19 (2000), S. 41-60 
    ISSN: 1573-1383
    Keywords: Codesign ; FPGA ; Spring Forming Machine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract A new approach to design a high precision real-time control system for a spring forming machine is presented. This system is designed to satisfy the required high motor speed command signal and the product to product tolerance is limited to 0.1%. An innovative variable pulse generation algorithm is proposed for the activation of the motor in order to meet the stringent specifications. Because of the high speed command, software implementation of this algorithm would not be viable as this is intended to be a low cost automation system. To further enhance the system performance, an overall system architecture to incorporate a hardware solution is proposed. This new architecture comprises hardware and software functionality such that the codesign methodology is employed for the partitioning of the design procedures. Within this architecture, software development includes a user-friendly, safety and fault detection facility to improve the human-machine interface. This new precision control system meets the required performance specifications with only two-pieces of 2K gates Field Programmable Gate Array (FPGA) Actel chips are required for the implementation.
    Type of Medium: Electronic Resource
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