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  • Springer  (56)
  • International Union of Crystallography  (10)
  • Blackwell Science Ltd
  • 2000-2004  (68)
  • 1990-1994
  • 1950-1954
  • 1935-1939
  • 2000  (68)
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  • 2000-2004  (68)
  • 1990-1994
  • 1950-1954
  • 1935-1939
Year
  • 1
    ISSN: 1432-1327
    Keywords: Metal substitution Cerium binding Lactoferrin Crystal structure Transferrin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. Proteins of the transferrin family play a key role in iron homeostasis through their extremely strong binding of iron, as Fe3+. They are nevertheless able to bind a surprisingly wide variety of other metal ions. To investigate how metal ions of different size, charge and coordination characteristics are accommodated, we have determined the crystal structure of human lactoferrin (Lf) complexed with Ce4+. The structure, refined at 2.2 Å resolution (R=20.2%, R free=25.7%) shows that the two Ce4+ ions occupy essentially the same positions as do Fe3+, and that the overall protein structure is unchanged; the same closed structure is formed for Ce2Lf as for Fe2Lf. The larger metal ion is accommodated by small shifts in the protein ligands, made possible by the presence of water molecules adjacent to each binding site. The two Ce4+ sites are equally occupied, indicating that the known difference in the pH-dependent release of Ce4+ arises from a specific protonation event, possibly of the His ligand in one of the binding sites. Comparing the effects of binding Ce4+ with those for the binding of other metal ions, we conclude that the ability of transferrins to accommodate metal ions other than Fe3+ depends on an interplay of charge, size, coordination and geometrical preferences of the bound metal ion. However, it is the ability to accept the six-coordinate, approximately octahedral, site provided by the protein that is of greatest importance.
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  • 2
    ISSN: 1572-9591
    Keywords: Fusion energy ; international collaboration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract This report was prepared by a Working Group at the request of the U.S. Department of Energy, Office of Fusion Energy Sciences in 1997. The report addresses technical opportunities for mutually beneficial collaboration between the United States and other international fusion research programs. A number of outstanding opportunities are discussed.
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  • 3
    Publication Date: 2000-08-25
    Print ISSN: 0108-7673
    Electronic ISSN: 2053-2733
    Topics: Chemistry and Pharmacology , Geosciences , Physics
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  • 4
  • 5
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effect of defoliation on the vegetative, early reproductive and inflorescence stages of tiller development, changes in the dry-matter yield of leaf, stem and inflorescence and the associated changes in forage quality was determined on plants of annual ryegrass (Lolium rigidum Gaud.) and Italian ryegrass (L. multiflorum Lam.). The field study comprised seventy-two plots of 1 m × 2 m, sown with one annual ryegrass and seven Italian ryegrass cultivars with a range of heading dates from early to late; defoliation commenced 6 weeks after germination. During the vegetative stage of growth, plots were defoliated when the tillers had three fully expanded leaves (three-leaf stage). During the early reproductive stage of growth, to simulate a cut for silage, plots were defoliated 6–7 weeks after 0·10 of the tillers displayed nodal development. The subsequent regrowth was defoliated every 3 weeks.Assessments of changes in tiller density, yield and quality were made in the growth cycle that followed three contrasting cutting treatments during the winter–spring period (from 10 July). In treatment 1, this growth cycle (following closing-up before a subsequent conservation cut) commenced on 7 August following two defoliations each taken when the tillers were at the three-leaf stage. In treatment 2, the growth cycle commenced on 16 October following: for early-maturing cultivars, two cuts at the three-leaf stage, a cut for silage and an additional regrowth cut; for medium-maturing cultivars three cuts at the three-leaf stage and a cut for silage; and late-maturing cultivars, five cuts at the three-leaf stage. In treatment 3, defoliation up to 16 October was as for treatment 2, but the growth cycle studied started on 27 November following two additional regrowth cuts for early- and medium-maturing cultivars and cut for silage for the late-maturing cultivars.Tiller development for all cultivars was classified into three stages; vegetative, early reproductive and inflorescence. In treatment 1, in vitro dry-matter digestibility (IVDMD) and crude protein (CP) content were negatively associated with maturation of tillers. IVDMD ranged from 0·85 to 0·60 and CP ranged from 200 to less than 100 g kg–1 dry matter (DM) during the vegetative and inflorescence stages respectively. This large reduction in forage quality was due to an increase in the proportion of stem, inflorescence and dead material, combined with a reduction in the IVDMD and CP content of the stem. A high level of forage quality was retained for longer with later-maturing cultivars, and/or when vegetative tillers were initiated from the defoliation of early reproductive tillers (treatments 2 and 3). However, 15 weeks after the closing-up date in treatment 1, defoliation significantly reduced the density of inflorescences with means (±pooled s.e_m.) of 1560, 1178 and 299 ± 108 tillers m–2, and DM yield of inflorescence with means of 3·0, 0·6 and 0·1 ± 0·15 t ha–1 for treatments 1, 2 and 3 respectively.This study supports the recommendation that annual and Italian ryegrass cultivars should be classified according to maturity date based on the onset of inflorescence emergence, and that the judicious defoliation of early reproductive tillers can be used to promote the initiation of new vegetative tillers which in turn will retain forage quality for longer.
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  • 6
    ISSN: 1365-2486
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: The purpose of this study was to test for direct inhibition of rice canopy apparent respiration by elevated atmospheric carbon dioxide concentration ([CO2]) across a range of short-term air temperature treatments. Rice (cv. IR-72) was grown in eight naturally sunlit, semiclosed, plant growth chambers at daytime [CO2] treatments of 350 and 700 μmol mol−1. Short-term night-time air temperature treatments ranged from 21 to 40 °C. Whole canopy respiration, expressed on a ground area basis (Rd), was measured at night by periodically venting the chambers with ambient air. This night-time chamber venting and resealing procedure produced a range of increasing chamber [CO2] which we used to test for potential inhibitory effects of rising [CO2] on Rd. A nitrous oxide leak detection system was used to correct Rd measurements for chamber leakage rate (L) and also to determine if apparent reductions in night-time Rd with rising [CO2] could be completely accounted for by L. The L was affected by both CO2 concentration gradient between the chamber and ambient air and the inherent leakiness of each individual chamber. Nevertheless, after correcting Rd for L, we detected a rapid and reversible, direct inhibition of Rd with rising chamber [CO2] for air temperatures above 21 °C. This effect was larger for the 350 compared with the 700 μmol mol−1 daytime [CO2] treatment and was also increased with increasing short-term air temperature treatments. However, little difference in Rd was found between the two daytime [CO2] treatments when night-time [CO2] was at the respective daytime [CO2]. These results suggest that naturally occurring diurnal changes in both ambient [CO2] and air temperature can affect Rd. Because naturally occurring diurnal changes in both [CO2] and air temperature can be expected in a future higher CO2 world, short-term direct effects of these environmental variables on rice Rd can also be expected.
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  • 7
    ISSN: 1476-5535
    Keywords: Keywords: echinocandin B deacylase; substrate specificity; evolution/technology; antifungal agent
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Aspergillus nidulans produces echinocandin B, a neutral lipopeptide. A deacylase from Actinoplanes utahensis catalyzes cleavage of the linoleoyl group from echinocandin B, a key step in generating a potential antifungal agent. Virtually all (99.8%) deacylase activity was cell-associated. The deacylase was salt-solubilized, heat-treated and purified to apparent homogeneity by a 3-step chromatographic procedure. The enzyme was a heterodimer consisting of 63- and 18-to-20-kDa subunit, optimally active at pH 6.0, and at 60°C with salt. The K m of the deacylase for echinocandin B was 50 μM and its V max was 14.6 μmol cyclic hexapeptide min−1 mg−1protein. The substrate specificity of the enzyme was broad with respect to both acyl and cyclic peptide analogues of echinocandin B. The two deacylase subunit genes were cloned and over-expressed in Streptomyces lividans. The recombinant deacylase was purified from the culture filtrate to apparent homogeneity by a 1-step chromatographic procedure. Using the recombinant deacylase, an enzymatic deacylation of immobilized echinocandin B resulted in the generation of cyclic hexapeptide at gram-level. Journal of Industrial Microbiology & Biotechnology (2000) 24, 173–180.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Research in engineering design 12 (2000), S. 61-72 
    ISSN: 1435-6066
    Keywords: Key words:Product families – Product platforms, Product portfolio architecture.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract: Consider a group of products sharing common parts and assemblies. The products in question we call a product family, and the common elements, the platform. In this paper, we present a method for designing product platforms and the derived family that takes into consideration both the technical performance requirements as well as the cost of the product family. The design of a platform-based product family is formulated as a general optimization problem in which the advantages of designing a common platform must be balanced against the constraints of the individual product variants and constraints of the family as a whole. This optimization approach forms the basis for a practical implementation as an interactive, team-based negotiation model for designing a family of interplanetary spacecraft based on a common platform. The approach is used to consider and specify different subsystems that could be made common to all the missions. It is also used to evaluate the impact of those platform design decisions on the performance of the product family, and thus be able to select from among feasible platform designs.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical crystallography 30 (2000), S. 181-184 
    ISSN: 1572-8854
    Keywords: tungsten(II) ; diiodo ; dicarbonyl ; triisopropylphosphite ; 3-hexyne ; crystal structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract [WI2(CO)2{P(OiPr)3}(η2-EtC2Et)] crystallizes in the monoclinic space group P21/n, with a = 11.101(12), b = 16.272(18), c = 14.892(17) Å, β = 93.27(1), Z = 4. The geometry can be considered to be pseudo-octahedral, with the 3-hexyne ligand occupying one site, with two iodo-groups, and the P(OiPr)3 ligand completing the equational plane of ligands, with two trans-carbonyl groups occupying the axial sites.
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  • 10
    ISSN: 1572-8927
    Keywords: Thermoelectric power ; tetra-n-butylammonium hydroxide ; heat of transport ; enthalpy of transport ; entropy of transport ; transported entropy ; thermocell ; hydrogen electrode
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Using a hydrogen-electrode thermocell with a temperature difference of 8 K, initialthermoelectric power εin has been determined in aqueous tetra-n-butylammoniumhydroxide solutions for concentrations ranging from 0.001 to 0.2 molal at meantemperatures of 25 and 35°C. Graphs of a function of εin vs. m 1/2/(1 + m 1/2) yieldintercepts and infinite-dilution limiting slopes as m 1/2 → 0. Using the value $$\overline{\overline S} _{{\text{OH}}}^{\text{o}} $$ = 68.8±0.3 J-K−1for the standard transported entropy of the hydroxideion, values for the enthalpy of transport of tetra-n-butylammonium hydroxide atinfinite dilution were determined to be 36.5±1.5 kJ-mol−1 at25° and 37.7±2.0kJ-mol−1 at 35°C.
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