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  • Blackwell Publishing Ltd  (36)
  • Oxford University Press  (28)
  • 2000-2004  (64)
  • 2004  (35)
  • 2000  (29)
  • 1
    Electronic Resource
    Electronic Resource
    Effect of Supplementing Ascorbic Acid (L-ascorbyl-2-polyphosphate) in Broodstock Diet of the White Shrimp Litopenaeus vannamei (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the World Aquaculture Society 31 (2000), S. 0 
    Details
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-7345.2000.tb00709.x
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  • 2
    Electronic Resource
    Electronic Resource
    Ribonuclease U2: cloning, production in Pichia pastoris and affinity chromatography purification of the active recombinant protein (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 189 (2000), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: RNase U2 is an endoribonuclease secreted by the fungus Ustilago sphaerogena. Its genomic DNA (rnu2), containing an intron of 116 bp, has been isolated and cloned. The corresponding cDNA has also been synthesized. The recombinant RNase U2 was successfully produced in Pichia pastoris, fused to the yeast alkaline phosphatase signal peptide. The recombinant RNase U2, purified by affinity chromatography, contains three extra amino acids at its amino-terminal end and retains the enzymatic and spectroscopic properties of the natural fungal protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2000.tb09224.x
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  • 3
    Electronic Resource
    Electronic Resource
    The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 191 (2000), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We report cloning and sequencing of gene ps1 encoding a versatile peroxidase combining catalytic properties of lignin peroxidase (LiP) and manganese peroxidase (MnP) isolated from lignocellulose cultures of the white-rot fungus Pleurotus eryngii. The gene contains 15 putative introns, and the deduced amino acid sequence consists of a 339-residue mature protein with a 31-residue signal peptide. Several putative response elements were identified in the promoter region. Amino acid residues involved in oxidation of Mn2+ and aromatic substrates by direct electron transfer to heme and long-range electron transfer from superficial residues as predicted by analogy with Phanerochaete chrysosporium MnP and LiP, respectively. A dendrogram is presented illustrating sequence relationships between 29 fungal peroxidases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2000.tb09316.x
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  • 4
    Electronic Resource
    Electronic Resource
    Residual effects of pig slurry applied to a Mediterranean soil on yield and N uptake of a subsequent wheat crop (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 20 (2004), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. In areas of intensive pig farming, fresh pig slurry is often applied annually to the same fields. Thus, to avoid nitrogen (N) losses correct fertilizer practice should take account of residual effects of slurry on the following crops. The residual effects of different rates of slurry applied during three years were evaluated in subsequent wheat crops. The experiment was conducted on an irrigated Mediterranean Typic Xerofluvent soil, where plots were left unfertilized or fertilized with 150 kg N ha−1 as ammonium nitrate. Grain yield and grain N uptake increased with slurry rates in both fertilized and unfertilized treatments. The increases in the unfertilized treatments were interpreted as a nitrogen effect of the previous 1996–98 slurry applications. The equivalent mineral N released from the pig slurry was underestimated by two existing decay-series approaches. Although decay-series are useful tools for estimating manure residual effects they should be adjusted for local conditions. A significant positive relationship was detected between apparent N use efficiency of the slurry and the total amount of applied organic N, which was interpreted as a specific residual effect rather than due to the N dose of previously applied pig slurry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2004.tb00351.x
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  • 5
    Electronic Resource
    Electronic Resource
    Nutrient fluxes from a soil treatment process for pig slurry (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 16 (2000), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. The effects of pig slurry applications to a hydrologically isolated field treatment plant (at Solepur) were studied over a period of eight years. Thirty repeated doses, averaging 160 m3 ha−1 were applied from April to October of each year (1991–1995), to reach a total application of 4930 m3 ha−1. All slurry samples were analysed for their total solids (TS), macronutrient (C, N, P, K, Ca) and micronutrient (Cu, Zn) content. In total, 284 tonnes of total solids (57 t TS ha−1 yr−1), 115 tonnes of carbon (23 t C ha−1yr−1), 24.5 tonnes of nitrogen (4900 kg N ha−1 yr−1), 7964 kg of phosphorus (1593 kg P ha−1 yr−1), 16 518 kg of potassium (3304 kg K ha−1 yr−1), 183 kg copper (37 kg Cu ha−1 yr−1) and 266 kg zinc (53 kg Zn ha−1 yr−1) were applied to the soil. Thus, this site provides an opportunity to assess the balance and to examine the long-term behaviour of nutrients under conditions of intensive land application of pig slurries or similar effluents.The main nutrient fluxes through the soil-water system were determined for each element. Over 40% of the total carbon applied was retained by the soil. About 25% of the slurry nitrogen applied remained in the soil profile and 12.5% was leached through the drainage water as nitrate. Most of the slurry phosphorus applied was retained in the soil profile either as P-Dyer extractable (83%), or as total soil phosphorus (112%); 〈0.01% was found in the drainage water. Forty-three per cent of the potassium applied in the slurry was recovered from the soil profile and 15% was recovered in the drainage water. Most of the copper (62%) and zinc (74%) applied in the slurry remained in the soil as EDTA extractractable forms; very low percentages (0.05% and 0.6% respectively) were found in the drainage water.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2000.tb00183.x
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  • 6
    Electronic Resource
    Electronic Resource
    Dissolved reactive phosphorus in a Calcaric Fluvisol as affected by the addition of agricultural wastes (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 20 (2004), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Land application of organic wastes can result in the accumulation of available soil phosphorus (P) and in an increase risk of eutrophication of surface and shallow groundwaters. We conducted laboratory experiments to examine the effect of waste application on the concentration of dissolved reactive phosphorus (DRP) in 1:5 soil to 0.005 m CaCl2 suspensions. Ten organic wastes, of which eight were mature composts, were applied to a P-rich Calcaric Fluvisol at rates ranging from 0 to 10% of soil by weight, and the difference in DRP concentration between suspensions containing waste and the untreated control (ΔDRP) was measured over a period of 300 days. In half of the suspensions of each waste–soil combination, 80 mg P (as KH2PO4) kg−1 soil was applied at day 14. Values of ΔDRP were generally positive, but a significant number of negative values were also recorded for some wastes and application rates, particularly at later sampling times if inorganic P had been added. Regression analyses revealed that ΔDRP (i) increased with increasing soluble reactive P at all times and (ii) increased in the short term, but decreased in the long term with increasing water soluble organic carbon in the waste. The fact that ΔDRP was sometimes negative for some waste types suggests that application of organic waste to soil does not necessarily increase eutrophication risks caused by soil P losses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2004.tb00339.x
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  • 7
    Electronic Resource
    Electronic Resource
    The Ultrastructure of the Cyst Wall of Giardia lamblia (2004)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 51 (2004), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Giardiasis is the most common human protozoal infection. In then: cystic phase, giardias are protected from the environment by a filamentous cyst wall made up of carbohydrates, proteins, and by two outer membranes separated from the plasma membrane of the parasite by a peripheral space. The present transmission electron microscope observations of G. lamblia cysts of human origin suggest that the extracellular peritrophic space originates from the growth, elongation, and fusion of large cytoplasmic vacuoles. As the large clear vacuoles grew in size, flattening against the inner face of the plasma membrane, they formed a single vacuole that surrounded the body of the parasite, eventually forming two outer membranes. In mature Giardia cysts, the original plasma membrane of the trophozoite becomes the outermost membrane of the cyst wall (CM1). The large vacuoles form a second membrane surrounding the cyst (CM2), and also form a third membrane (CM3), that becomes the new plasma membrane of the trophozoite. During excystation CM1 and CM2 attach to each other and fragment, leaving abundant membrane residues in the peritrophic space. Knowledge of the biochemical composition and functional properties of the complex outer membranous system of G. lamblia cysts here described will be of use to understand the survival of Giardia cysts in the environment, a major factor responsible for the high prevalence of giardiasis worldwide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.2004.tb00549.x
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  • 8
    Electronic Resource
    Electronic Resource
    Novel variation for the tocopherol profile in a sunflower created by mutagenesis and recombination (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: α-Tocopherol is the main tocopherol in sunflower seeds (〉90%). Because it exerts a weak antioxidant action in vitro, its partial replacement by other tocopherols is an important breeding objective in this crop. The objective of this research was to develop novel tocopherol profiles in sunflower through mutagenesis and genetic recombination. Seeds of four ‘Peredovik’ accessions were used for chemical mutagenesis with ethyl methane sulfonate (EMS). Single-seed screening in the M2 generation resulted in two M2 seeds, derived from different M1 plants, with increased γ-tocopherol contents of 19.2% and 96.7%, respectively. M3 progeny from the M2 seed with the 96.7% content bred true for high c-tocopherol content, containing more than 90%γ-tocopherol. M3 progeny from the M2 seed with only 19.2%γ-tocopherol segregated in a range from 0 to 84.6%. Selection for high c-tocopherol content produced an M4:5 line, designated IAST-1, with a stable high concentration of γ-tocopherol. Crosses between IAST-1 and T589, with an increased b-tocopherol content, produced F2 segregants with trans-gressive levels of up to 77%β-tocopherol or up to 68% d-tocopherol. Both novel tocopherol profiles were confirmed in the F3 generation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2004.01012.x
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  • 9
    Electronic Resource
    Electronic Resource
    Breeding for resistance to begomovirus in tropic-adapted tomato genotypes (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Four tomato lines introgressed from Lycopersicon chilense were compared with the commercial F1 hybrids ‘ARO 8479’ and ‘HA 3108’, which are tolerant to Tomato yellow leaf curl virus, and the cv. ‘Campbell 28’ as a susceptible control. Resistance was evaluated by the use of grafted diseased scions as well as in a field trial where plants infected by viruliferous whiteflies and disease-free plants were transplanted in paired rows. The new lines LD 3, LD 4, LD 5 and LD 6 showed no disease symptoms after grafting or in the field trial. Virus accumulation at 60 days after transplanting was low in the infected plants: 0.09, 0.60, 1.00 and 0.50 ng, respectively. No fruit-set or yield losses were registered under the high temperature conditions prevalent in the trial, in which lines LD 5 and LD 6 were better adapted to tropical conditions. Viral DNA concentrations were over 1000 ng in the cvs.‘Campbell 28′,‘ARO 8479’ and ‘HA 3108’. The last two are considered tolerant as they were asymptomatic or had mild symptoms, respectively, but achieved acceptable yields in the trial. By contrast, virus had a negative effect on fruit-set, number of fruit per plant and total yield in the cv.‘Campbell 28’.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2004.00959.x
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  • 10
    Electronic Resource
    Electronic Resource
    1-MCP Preserves the Firmness of Stored Persimmon ‘Rojo Brillante’ (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 69 (2004), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : We studied the effects of 1-methylcyclopropene (1-MCP), an ethylene inhibitor, on quality of persimmon cv.‘Rojo Brillante.’Two assays were carried out, applying deastringency treatment before or after storage at 15 °C. In both experiments, fruit was treated with 0 (control), 0.5, or 1 μL/L of 1-MCP after harvest. Additionally, the effect of 2 applications of 0.5 μ L/L, one after harvest and the 2nd after a storage period, was evaluated. In all experiments, 1-MCP delayed softening and color evolution of persimmons. Ethanol and acetaldehyde levels in treated fruit were lower than those in control fruit. 1-MCP had no effects on weight loss, °Brix, or ethylene and CO2 production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2004.tb15516.x
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