ISSN:
1432-203X
Keywords:
Key words Barley
;
Desiccation
;
Embryos
;
Doubled haploids
;
Microspores
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Barley microspore-derived doubled-haploid embryos have been produced in vitro. The development of embryo desiccation technology will allow long-term storage, germplasm preservation and low delivery cost. Treatment of the microspore-derived embryos was essential to induce desiccation tolerance and to arrest further development and plant regeneration. At the concentrations used, a treatment with trehalose was more efficient than with sucrose, and mannitol was harmful to the embryos. Up to 80% of the desiccated embryos produced complete green plants when transferred to regeneration medium, by the application of a 0.6 m trehalose or a 10–5 m abscisic acid treatment to the embryos in the culture induction medium. The morphology of these plants was similar to plants produced directly from non-desiccated embryos.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050685
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