ISSN:
1432-072X
Keywords:
Key words Chaperone
;
Cyanobacteria
;
groEL
;
isiA
;
RNA helicase
;
Salt stress
;
Subtractive RNA ¶hybridization
;
Synechocystis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract To identify genes transcribed preferentially under salt stress, a subtractive RNA hybridization procedure was applied to the cyanobacterium Synechocystis sp. PCC 6803. The screening of a genomic library led to the identification of several RNA species that were more abundant in salt-stressed cells than in control cells. Salt-dependent transcription of the identified genes was verified in Northern blot experiments. In addition to the previously characterized genes cpn60 (encoding GroEL; a molecular chaperone) and isiA (encoding a chlorophyll-binding protein), genes encoding a protein of unknown function (slr0082) and a putative RNA helicase (slr0083) were identified as salt-regulated genes in Synechocystis. Genes slr0082 and slr0083, located at sites adjacent to each other on the Synechocystis chromosome, were transcribed from separate promoters and showed the most significant induction 1–3 h after salt shock. The salt-regulated promoters of these two genes were mapped. Genes cpn60, slr0082, and slr0083 were also found to be induced by a cold shock. The possible role of the identified gene products for salt adaptation of Synechocystis is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002030050774
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