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  • Articles  (38)
  • 2020-2024
  • 1995-1999  (38)
  • 1955-1959
  • 1940-1944
  • 1999  (38)
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  • 2020-2024
  • 1995-1999  (38)
  • 1955-1959
  • 1940-1944
Year
Journal
Topic
  • 1
    Electronic Resource
    Electronic Resource
    The role of Pseudomonas aeruginosa elastase as a potent inflammatory factor in a rat air pouch inflammation model (1999)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS immunology and medical microbiology 25 (1999), S. 0 
    Details
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Pseudomonas aeruginosa, an opportunistic pathogen, can cause life threatening infections in patients compromised by underlying respiratory disease like bronchiectasis, cystic fibrosis and diffuse panbronchiolitis. Most strains of P. aeruginosa produce some kind of protease with broad substrate specificities during the infectious state in the host. P. aeruginosa elastase, one of the strongest exotoxins, has a tissue-damaging proteolytic activity and is capable of degrading such plasma proteins as immunoglobulins, complement factor and cytokines. The present study focused on the effect of P. aeruginosa elastase and was designed to evaluate the neutrophil accumulation at the inflammation site mediated by P. aeruginosa elastase in the inflammatory response in the host. An air pouch model in rats, considered as a useful model of inflammation, was used to analyze the number of leukocytes, the volume of exudate and the concentration of interleukin-8 after the injection of P. aeruginosa elastase into the pouch cavity. The number of neutrophils and the volume of exudate in the pouch cavity increased significantly at 4 h, peaked at 8 h in a dose-dependent manner and then decreased at 24 h. The concentration of interleukine-8 in pouch fluid peaked 4 h earlier than the peak of the neutrophil number. The enzymatic activity of P. aeruginosa elastase seemed to reinforce the inflammation process. The influence of lipopolysaccharide contamination was negligible. Although these observations were made in the subcutaneous cavity, they indicate that P. aeruginosa elastase plays a role as an immunoprovocative factor in the inflammatory response in cases of infection with P. aeruginosa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-695X.1999.tb01356.x
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  • 2
    Electronic Resource
    Electronic Resource
    Reconstruction of Extensive Long-Bone Defects in Sheep Using Porous Hydroxyapatite Sponges (1999)
    Springer
    Calcified tissue international 64 (1999), S. 83-90 
    Details
    ISSN: 1432-0827
    Keywords: Key words: Hydroxyapatite — Ceramics — Bone reconstruction — Bone repair — Biomaterials — Orthopedic surgery.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. The capacity of hydroxyapatite (HA) implants to support large defect repair in weight-bearing long bones of large size animals was investigated. Diaphyseal resections 3.5 cm of the tibia were performed in five adult sheep. They were substituted with HA macroporous ceramic cylinders anatomically shaped, and an external fixator was assembled. The sheep were sacrificed at 20, 40, 60, 120, and 270 days after surgery, respectively. Histology and micro X-ray study of resected implants and adjacent tissues showed proper integration of ceramic with newly formed periosteal bone as early as 20 days after surgery. In one sheep, the external fixator was removed 5 months after surgery. The animal gained the ability to walk with no functional impairment until it was sacrificed 4 months later. At this time, extensive integration of ceramic with bone was detected radiographically and confirmed by a morphological study of the resected sample. Our data indicate that large defects in a weight-bearing long bone can be repaired to the extent necessary for full functional recovery in large animals. These data set the stage for further intervention on material properties as well as for preliminary attempts to use ceramic prostheses for reconstruction of large bone defects in humans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002239900583
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  • 3
    Electronic Resource
    Electronic Resource
    Metaphase-specific cell death in meiotic spermatocytes in mice (1999)
    Springer
    Cell & tissue research 296 (1999), S. 359-369 
    Details
    ISSN: 1432-0878
    Keywords: Key words Apoptosis ; Electron microscopy ; Meiosis ; Spermatocytes ; Spermatogenesis ; Testis ; TUNEL ; Mouse (10 strains)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Apoptosis of male germ cells is a widespread but little-understood phenomenon in many animal species. The elucidation of its mechanisms could be useful in the understanding of male infertility. We have examined the distribution of dying cells with the terminal transferase-mediated nick-end labeling (TUNEL) method and by an electron-microscopic procedure in the testes of 10 mouse strains, viz., C57BL/10 (B10), SL/NiA (SL), C57BL/6 (B6), C3H/He (C3H), BALB/c (BALB), DBA2 (DBA), CBA/J (CBA), MRL/MpJ-+/+ (M+), MRL/MpJ-lpr/lpr (lpr), and wild-type NJL mice (Mus musculus musculus). In the testes of the B10, NJL, SL, B6, C3H, BALB, DBA, and CBA mice, very few TUNEL-positive cells are distributed in the seminiferous tubules, whereas in the testes of the M+ and lpr mice, many TUNEL-positive cells, which are restricted to stage XII seminiferous tubules, have been identified. The most important finding is that many metaphases of meiotic spermatocytes show a marked TUNEL-positive reaction. Some metaphases show apoptotic morphology electron-microscopically. These results suggest that the testes of MRL strains will provide a useful model for the study of the mechanism of metaphase-specific apoptosis in meiotic spermatocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410051296
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  • 4
    Electronic Resource
    Electronic Resource
    Subcellular targeting of green fluorescent protein to plastids in transgenic rice plants provides a high-level expression system (1999)
    Springer
    Molecular breeding 5 (1999), S. 453-461 
    Details
    ISSN: 1572-9788
    Keywords: transgenic rice ; transit peptide ; plastid targeting ; green fluorescent protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In order to develop a high-level expression system in transgenic rice, we inserted a synthetic gene (sgfp) encoding a modified form of the green fluorescent protein (GFP) into two expression vectors, Act1-sgfp for an untargeted and rbcS-Tp-sgfp for a chloroplast targeted expression. Several fertile transgenic rice plants were produced by the Agrobacterium-mediated method. Confocal microscopic analyses demonstrated that, in cells expressing the Act1-sgfp, GFP fluorescence was localized within the cytoplasm and nucleoplasm whereas, in cells expressing the rbcS-Tp-sgfp fusion gene, the fluorescence was specifically targeted to chloroplasts and non-green plastids. The levels of sgfp expression were about 0.5% of the total soluble protein in mature leaf tissues of the Act1-sgfp transformed lines. In contrast, expression levels were markedly increased in mature leaf tissues of the rbcS-Tp-sgfp transformed lines, yielding about 10% of the total soluble protein. N-terminal sequencing of the localized GFPs revealed that the Tp-GFP fusion protein was correctly processed during import to non-green plastids, as well as to chloroplasts. Thus, our results demonstrate that GFP can be produced at high levels and localized in specific subcellular spaces of transgenic plants, providing a high-level expression system for general use in rice, an agronomically important cereal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009665314850
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  • 5
    Electronic Resource
    Electronic Resource
    Molecular and genetic analysis of transgenic rice plants expressing the maize ribosome-inactivating protein b-32 gene and the herbicide resistance bar gene (1999)
    Springer
    Molecular breeding 5 (1999), S. 85-94 
    Details
    ISSN: 1572-9788
    Keywords: transgenic rice ; bar ; b-32 ; proteolytic processing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We have previously transformed rice (Oyrza sativa L.) with the maize ribosome-inactivating protein b-32 gene (Zmcrip3a) and the phosphinothricin resistance gene (bar). In the present study, Southern blot hybridization analysis of 56 primary fertile transformants resulted in distinct band patterns, indicating that all the transformants had been generated by independent integration events and 30% of them contained a single copy of the transgene. Segregation analysis of 15 R0 plants revealed that transgene was stably transmitted to their progenies and Southern blot band patterns of R1 progenies remained the same as the corresponding parents, suggesting that all the loci of multiple integration events are genetically linked. Also, in most of the lines, physical presence of the b-32 transgene co-segregated with the phosphinothricin- resistant phenotype, confirming that the transgene is behaving as a normal locus in the genome. However, some of R1 seedlings that contained multiple copies of the transgene became sensitive to phosphinothricin, indicating that its expression was silenced. Immunoblot analysis demonstrated that b-32 protein was produced and the levels of expression differed in different lines, estimated to be 0.5–1% of total soluble protein in the leaf tissues. In addition, the transgene-encoded protein was preferentially processed in germinating seeds and young leaves of R2 transgenic plants in a way similar to that in maize kernels, suggesting that the processing mechanism is conserved in the germination stage between rice and maize.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009692230725
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  • 6
    Electronic Resource
    Electronic Resource
    Associated stop-Higgs production at a linear collider and extraction of stop parameters (1999)
    Springer
    The European physical journal 9 (1999), S. 511-520 
    Details
    ISSN: 1434-6052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract. We calculate stop-stop-Higgs production at a linear collider. Combining the measurements from the pair production of the lightest stop and that of the mass of the Higgs, we show how, in a scenario where only the lightest stop and the lightest Higgs are accessible, one could extract the mass of the heavier stop and infer some useful information on the supersymmetric parameters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100520050045
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  • 7
    Electronic Resource
    Electronic Resource
    Jumbled spine and ribs (Jsr): a new mutation on mouse Chromosome 5 (1999)
    Springer
    Mammalian genome 10 (1999), S. 213-217 
    Details
    ISSN: 1432-1777
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Jumbled spine and ribs (Jsr) is an autosomal dominant mutation that results in malformation of the axial skeleton. The vertebrae of mutant mice (Jsr/+) are all shorter than those of normal mice (+/+) in the inbred line and show various abnormalities. In addition, several ribs are fused at their proximal region because of fusion of thoracic vertebrae. In this study, we localized the Jsr mutation on distal Chromosome (Chr) 5 and constructed a high-resolution map. Chromosomal mapping was performed with an inter-subspecific backcross of (CKH-Jsr/+× MOG) F1 carrying the Jsr allele and CKH-+/+. The predicted gene order around Jsr was determined to be cen–(Epo, Pdgfa, D5Mit31, D5Mit374)–(Jsr, Nfe2u, D5Mit99, D5Mit247, D5Mit284, D5Mit292, D5Mit327)–D5Mit328–tel. Subsequently, high-resolution mapping concluded the Jsr localization to be cen–Nfe2u–1.0cM–Jsr–0.2cM–D5Mit247,292–tel. Jsr/Jsr homozygotes are alive, as the mutation is not lethal. Based on histological analysis of mutant embryos, Jsr is hypothesized to be caused by abnormal development of primordial cells in the axial skeleton.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003359900975
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  • 8
    Electronic Resource
    Electronic Resource
    Separation of Positional Isomers on A, C- and A,D-Bridged Calix[6]arene as Stationary Phases in Capillary GC (1999)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 22 (1999), S. 679-682 
    Details
    ISSN: 0935-6304
    Keywords: A,C- and A,D-bridged calix[6]arene ; stationary phase ; capillary gas chromatography ; geometric and positional isomer separation ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---A,C-Bridged (ACCX) and A,D-bridged isopropyldimethylsilylcalix[6]arene (ADCX) dissolved in OV-1701 were used as stationary phases in isothermal capillary gas chromatographic separation of some positional isomers. Retention factors and separation factors for the isomers were measured. The isomers investigated are well resolved on the two phases. Retention of all the solutes investigated is longer on ACCX than on ADCX. The longer retention on A,C-bridged calix[6]arene is probably due to extra inductive interactions of the solute molecule with the carbonyl moieties in the phase. Separation factors for closely eluting isomer pairs are similar on the two phases. This seems to indicate that the carbonyl moieties do not play an appreciable role in discriminating the isomer molecules on entering the cavity of the calixarene if the solute is retained by the inclusion process.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
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  • 9
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    Evaluation of the in Vivo Biodistribution of Indium-111 and Yttrium-88 Labeled Dendrimer-1B4M-DTPA and Its Conjugation with Anti-Tac Monoclonal Antibody† (1999)
    American Chemical Society
    Details
    Publication Date: 1999-01-01
    Print ISSN: 1043-1802
    Electronic ISSN: 1520-4812
    Topics: Chemistry and Pharmacology
    Published by American Chemical Society
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  • 10
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    Metaphase-specific cell death in meiotic spermatocytes in mice (1999)
    Springer
    Details
    Publication Date: 1999-04-21
    Print ISSN: 0302-766X
    Electronic ISSN: 1432-0878
    Topics: Biology , Medicine
    Published by Springer
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