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Digitale Medien

Biotransformation of 1-nitrobenzo[e]pyrene by the fungus Cunninghamella elegans (1999)

Pothuluri, J V ; Freeman, J P ; Fu, P P ; [weitere]

Springer

Journal of industrial microbiology and biotechnology 22 (1999), S. 52-57

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ISSN: 1476-5535

Schlagwort(e): Keywords: nitro-PAHs; metabolism; Cunninghamella elegans; biotransformation

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Biotransformation of 1-nitrobenzo[e]pyrene (1-nitro-BeP), an environmental pollutant derived from the nitration of a non-carcinogen, benzo[e]pyrene, was studied using the fungus Cunninghamella elegans ATCC 36112. After 72 h incubation, 89% of 1-nitro-[3H]BeP added had been metabolized to two major metabolites. These metabolites were separated by reversed-phase high performance liquid chromatography and identified by 1H NMR, UV-visible, and mass spectral techniques as 1-nitro-6-benzo[e]pyrenylsulfate and 1-nitrobenzo[e]pyrene 6-O-β-glucopyranoside. Comparison of the fungal metabolism patterns of 1-nitro-BeP and BeP indicates that the nitro group at the C-1 position of BeP altered the regioselectivity of metabolism.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1038/sj.jim.2900601

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Digitale Medien

Molecular characterisation of a NADH ubiquinone oxidoreductase subunit 5 from Schistosoma mansoni and inhibition of mitochondrial respiratory chain function by testosterone (1999)

Fantappié, Marcelo Rosado ; Galina, Antônio ; de Mendonça, Ricardo Luís ; [weitere]

Springer

Molecular and cellular biochemistry 202 (1999), S. 149-158

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ISSN: 1573-4919

Schlagwort(e): NADH Q oxidoreductase ; S. mansoni ; testosterone ; mitochondria

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Chemie und Pharmazie , Medizin

Notizen: Abstract Complementary DNA, encoding the mitochondrial enzyme NADH-ubiquinone oxidoreductase subunit 5 (SmND5) of the human parasite Schistosoma mansoni was isolated by screening an S. mansoni cDNA library with a human androgen receptor (hAR) cDNA probe. The complete nucleotide and deduced aminoacid sequences of SmND5 were determined. Southern blot analysis revealed the occurrence of a single copy gene for SmND5 and by means of RT-PCR, it was shown that sex- and stage-specific expression of SmND5 occurred. In order to establish a functional relationship between the mitochondrial enzyme and the androgen receptor, the effects of testosterone were compared to those of classical respiratory chain inhibitors, using adult schistosome and beef heart submitochondrial particles. Physiological concentrations of testosterone were able to inhibit the maintenance of proton gradient across the mitochondrial membranes, as well as ATP synthesis. The steroid was found to be cytotoxic to the larvae, but not to adult schistosomes. A model is proposed to explain the observed in vivo testosterone-related differences in worm burdens, in experimental chronic infections.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1007057903390

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