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  • nitrate  (2)
  • Keywords: nitro-PAHs; metabolism; Cunninghamella elegans; biotransformation  (1)
  • S. mansoni  (1)
  • 2020-2022
  • 1995-1999  (4)
  • 1999  (4)
Collection
Publisher
Years
  • 2020-2022
  • 1995-1999  (4)
Year
  • 1999  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 22 (1999), S. 52-57 
    ISSN: 1476-5535
    Keywords: Keywords: nitro-PAHs; metabolism; Cunninghamella elegans; biotransformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Biotransformation of 1-nitrobenzo[e]pyrene (1-nitro-BeP), an environmental pollutant derived from the nitration of a non-carcinogen, benzo[e]pyrene, was studied using the fungus Cunninghamella elegans ATCC 36112. After 72 h incubation, 89% of 1-nitro-[3H]BeP added had been metabolized to two major metabolites. These metabolites were separated by reversed-phase high performance liquid chromatography and identified by 1H NMR, UV-visible, and mass spectral techniques as 1-nitro-6-benzo[e]pyrenylsulfate and 1-nitrobenzo[e]pyrene 6-O-β-glucopyranoside. Comparison of the fungal metabolism patterns of 1-nitro-BeP and BeP indicates that the nitro group at the C-1 position of BeP altered the regioselectivity of metabolism.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-4919
    Keywords: NADH Q oxidoreductase ; S. mansoni ; testosterone ; mitochondria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Complementary DNA, encoding the mitochondrial enzyme NADH-ubiquinone oxidoreductase subunit 5 (SmND5) of the human parasite Schistosoma mansoni was isolated by screening an S. mansoni cDNA library with a human androgen receptor (hAR) cDNA probe. The complete nucleotide and deduced aminoacid sequences of SmND5 were determined. Southern blot analysis revealed the occurrence of a single copy gene for SmND5 and by means of RT-PCR, it was shown that sex- and stage-specific expression of SmND5 occurred. In order to establish a functional relationship between the mitochondrial enzyme and the androgen receptor, the effects of testosterone were compared to those of classical respiratory chain inhibitors, using adult schistosome and beef heart submitochondrial particles. Physiological concentrations of testosterone were able to inhibit the maintenance of proton gradient across the mitochondrial membranes, as well as ATP synthesis. The steroid was found to be cytotoxic to the larvae, but not to adult schistosomes. A model is proposed to explain the observed in vivo testosterone-related differences in worm burdens, in experimental chronic infections.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-515X
    Keywords: ammonium ; climate change ; drought ; groundwater table ; nitrate ; nitrous oxide ; wetland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Certain general circulation models predict that a doubling of atmospheric carbon dioxide concentrations will increase the frequency of summer drought in northern wetlands due to hotter, drier summers. There is currently much uncertainty as to how drought will affect emissions of the greenhouse gas, nitrous oxide, from wetlands. We have demonstrated that an eight centimetre drawdown of the water table in a gully mire does not significantly affect nitrous oxide emissions from this site. However, under a more extreme drought scenario carried out on peat monoliths, nitrous oxide emissions increased exponentially with a linear decrease in water table height. Drought caused a significant increase in nitrous oxide production below the water table but most of the total increase could be attributed to increased emissions above the water table. Results from an acetylene block experiment suggested that increased emissions were caused by increased nitrous oxide production from denitrification, rather than by increased production from nitrification. In the laboratory study, drought severity had no effect on peatwater nitrate concentrations below the water table, however, increasing drought severity decreased ammonium concentrations.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-515X
    Keywords: ammonium ; climate change ; drought ; groundwater table ; nitrate ; nitrous oxide ; wetland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Certain general circulation models predict that a doubling of atmospheric carbon dioxide concentrations will increase the frequency of summer drought in northern wetlands due to hotter, drier summers. There is currently much uncertainty as to how drought will affect emissions of the greenhouse gas, nitrous oxide, from wetlands. We have demonstrated that an eight centimetre drawdown of the water table in a gully mire does not significantly affect nitrous oxide emissions from this site. However, under a more extreme drought scenario carried out on peat monoliths, nitrous oxide emissions increased exponentially with a linear decrease in water table height. Drought caused a significant increase in nitrous oxide productionbelow the water table but most of the total increase could be attributed to increased emissionsabove the water table. Results from an acetylene block experiment suggested that increased emissions were caused by increased nitrous oxide production from denitrification, rather than by increased production from nitrification. In the laboratory study, drought severity had no effect on peatwater nitrate concentrations below the water table, however, increasing drought severity decreased ammonium concentrations.
    Type of Medium: Electronic Resource
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