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  • American Institute of Physics  (47)
  • Wiley-Blackwell  (12)
  • Oxford University Press  (10)
  • Blackwell Publishing Ltd  (4)
  • International Union of Crystallography  (4)
  • Hamburg: Verlag Weltarchiv
  • Nature Publishing Group
  • 1995-1999  (77)
  • 1970-1974
  • 1998  (77)
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  • 1995-1999  (77)
  • 1970-1974
Year
  • 1
    Electronic Resource
    Electronic Resource
    POWER AND SENSITIVITY OF THE SAME-DIFFERENT TEST: COMPARISON WITH TRIANGLE AND DUO-TRIO METHODS (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 13 (1998), S. 0 
    Details
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Same-different, duo-trio and triangle discrimination methods were compared using vanilla flavored yogurt with and without added sugar as the medium. Two experiments were performed, one in controlled laboratory conditions and the other in conditions approximating more to consumer testing. A modification of the same-different test had greater power than the duo-trio of triangle tests. At higher sugar concentrations, d’values for the three methods were equivalent. Yet, at lower sugar concentrations, the same-different d’tended to be higher. The results are discussed in terms of Sequential Sensitivity Analysis, memory effects and changes between β and criteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-459X.1998.tb00080.x
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  • 2
    Electronic Resource
    Electronic Resource
    Differential stress gene expression during the development of Neurospora crassa and other fungi (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 168 (1998), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Stress genes are differentially expressed during the development of Neurospora crassa and other fungi. Large amounts of constitutive heat shock protein 70 (HSC70) are found in dormant conidia of N. crassa, whereas little mRNA of the related glucose-regulated protein (grp78) is detected. It is, however, not generally clear whether heat shock protein or mRNA is preferentially stored in dormant spores. Germinating spores of N. crassa increase the level of grp mRNA. During this developmental stage, the induction of inducible heat shock protein (hsp) genes can be elicited by heat shock only at certain times after the beginning of germination. Exponential growth (proliferation) is paralleled by increased levels of HSCs. The stationary state is characterized by decreased levels of some HSCs and increased levels of others. Conidiation in N. crassa is accompanied by a strong enhancement of the synthesis and levels of HSCs but also of HSPs after heat shock. This increase may serve a need for additional rounds of replication, for the expression and transport of sporulation-specific proteins or for stabilization of macromolecules in the spores and their preservation for germination. The control mechanisms involved in the differential expression of hsc genes are currently not known.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1998.tb13268.x
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  • 3
    Electronic Resource
    Electronic Resource
    Quorum-sensing and siderophore biosynthesis in Pseudomonas aeruginosa: lasRllasI mutants exhibit reduced pyoverdine biosynthesis (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 166 (1998), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cell density-dependent gene expression in Pseudomonas aeruginosa is controlled, in part, by the quorum-sensing regulator LasR. lasR null mutants exhibited a reproducible 2-fold decrease in production of the catecholate-hydroxamate siderophore pyoverdine during grown under iron-limiting conditions. Similarly, lasI mutants defective in the biosynthesis of the autoinducer PAI-1 also exhibited a 2-fold decrease in pyoverdine production which could be largely restored upon addition of exogenous PAI-1. lasR mutants were not altered with respect to expression of the pvdD gene involved in the synthesis of the peptide portion of pyoverdine, indicating that some other pyoverdine biosynthetic gene(s) were affected by the LasRI status of the cell. This represents the first report of quorum-sensing regulation of siderophore production in bacteria and highlights the fact that cell density, while not an essential signal for pyoverdine expression, does enhance production of this siderophore.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1998.tb13910.x
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  • 4
    Electronic Resource
    Electronic Resource
    Membrane d-lactate oxidase in Zymomonas mobilis: evidence for a branched respiratory chain (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 168 (1998), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Respiratory chain composition of the ethanol-producing bacterium Zymomonas mobilis was studied. Its membrane d-lactate oxidase was characterised. With NADH, but not d-lactate as substrate, a cytochrome o-like component was seen in CO difference spectra. Chlorpromazine specifically inhibited reduction of cytochrome d, while myxothiazol eliminated the cytochrome o-like features in CO difference spectra. It is suggested that electrons from NADH are distributed between branches terminated by the cytochrome o-like component, cytochrome a, and cytochrome d. With d-lactate, electrons are transported to cytochrome a, or an unidentified CN−-sensitive oxidase, and cytochrome d.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1998.tb13260.x
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  • 5
    Electronic Resource
    Electronic Resource
    Supramolecular structures of poly(p-phenylenes) with oxyethylene side chains and their mixtures with lithium salts (1998)
    Weinheim : Wiley-Blackwell
    Macromolecular Chemistry and Physics 199 (1998), S. 2129-2140 
    Details
    ISSN: 1022-1352
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: A series of poly(p-phenylenes) with linear and branched oxyethylene side chains was synthesized. Microphase separation in the solid state causes polymers with linear side chains to form supramolecular structures, which consist of main chain layers separated by an amorphous side chain matrix. If mixed with lithium salts such as lithium triflate (LiOTf) or lithium bis(trifluoromethanesulfone)imide (LiNTf), the polymers form solid solutions. The experimental data suggest that the layered structures undergo a one-dimensional swelling in the direction perpendicular to the main chain layers when blended with LiOTf and that this salt is only incorporated into the side chain matrix. The observed melting point depression in polymer-LiNTf mixtures is explained by the assumption that the salt species are incorporated both into the side chain and the main chain microphase.
    Additional Material: 10 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    Dynamic determination of anaerobic acetate kinetics using membrane mass spectrometry (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 57 (1998), S. 127-135 
    Details
    ISSN: 0006-3592
    Keywords: membrane mass spectrometer ; kinetic measurements ; anaerobic biofilm ; acetate ; inhibition ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A small, stirred, 14.4-mL tank reactor was designed to serve as a measurement cell for short-term investigation of microbial kinetics. A mass spectrometer membrane probe allowed the measurement of the dissolved gases of hydrogen, methane, oxygen, and carbon dioxide. pH was measured by an electrode and controlled by addition of acid or alkali. The highly sensitive measurement of gases with low solubility allowed rapid measurements at very low conversion. In kinetic experiments, a stepwise increase of substrate concentration (method A) and continuous feed of substrate (method B) were used, allowing quick estimation of substrate kinetics. Acetate conversion in mixed culture biofilms from a fluidized bed reactor was investigated. Substrate inhibition was found to be negligible in the concentration range studied. Experiments at various pH values showed that the undissociated acid form was the kinetic determinant. Kinetic parameters for Haldane kinetics of protons were KSH = 1.3 × 10-5 mol m-3 and KIH = 8.1 × 10-3 mol m-3. With free acid (HAc) as the rate determining species, the kinetic parameters for method A were KSHAc = 0.005 mol m-3 and KIHAc = 100 mol m-3 and for method B were KSHAc = 0.2 mol m-3 and KIHAc = 50 mol m-3. The maximum biomass activity occurred at around pH 6.5. Acetate was exclusively converted to methane and CO2 at pH 〉 6. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 57: 127-135, 1998.
    Additional Material: 6 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    Analysis and simulation of complex interactions during dynamic microfiltration of Escherichia coli suspensions (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 59 (1998), S. 189-202 
    Details
    ISSN: 0006-3592
    Keywords: artificial neural network (ANN) ; microfiltration ; cell harvesting ; membrane fouling ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Microfiltration is an important unit operation in downstream processing. However, due to the influence of membrane fouling, prediction of the filtration performance for biological suspensions is difficult. This paper describes a modeling approach that allows a comprehensive description of filtration performance. On the basis of experimental data and linguistic information, a specific artificial neural network was developed that predicts the process behavior within a certain range of parameters. This approach allows us to analyze influences of fermentation on filtration. By using extensive simulations, the interactions of 17 parameters were examined and the fouling causes determined. The model was developed for cell harvesting of Escherichia coli through a shear-enhanced module. The method can be applied to any cross-flow filtration process. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:189-202, 1998.
    Additional Material: 15 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of epothilones (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 58 (1998), S. 554-559 
    Details
    ISSN: 0006-3592
    Keywords: directed evolution ; esterase ; epothilon ; Pseudomonas fluorescens ; stereoselectivity ; mutator strain ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The directed evolution of an esterase from Pseudomonas fluorescens using the mutator strain Epicurian coli XL1-Red was investigated. Mutants were assayed for their ability to hydrolyze a sterically hindered 3-hydroxy ester, which can serve as a building block in the synthesis of epothilones. Screening was performed by plating esterase producing colonies derived from mutation cycles onto minimal media agar plates containing indicator substances (neutral red and crystal violet). Esterase-catalyzed hydrolysis of the 3-hydroxy ester (ethyl or glycerol ester) was detected by the formation of a red color due to a pH decrease caused by the released acid. Esterases isolated from positive clones were used in preparative biotransformations of the ethyl ester. One variant containing two mutations (A209D and L181V) stereoselectively hydrolyzed the ethyl ester resulting in 25% ee for the remaining ester. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58: 554-559, 1998.
    Additional Material: 2 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    Phase separation in random copolymers from high conversion free radical copolymerization, 1Dedicated to Prof. Dr. W. Heitz on the occasion of his 65th birthday (1998)
    Weinheim : Wiley-Blackwell
    Macromolecular Chemistry and Physics 199 (1998), S. 735-744 
    Details
    ISSN: 1022-1352
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The phase separation of random copolymers during free radical copolymerization to high conversion was studied. In order to prepare in situ high impact thermoplastics during the copolymerization process, the attention was focussed on systems in which the more reactive comonomers form thermoplastics, whereas the less reactive components form elastomeric homopolymers. The studied systems (A, B) were (AN, EA), (AN, VA), (CHMA, MA) and (S, BA) (AN: acrylonitrile, EA: ethyl acrylate, VA: vinyl acetate, CHMA: cyclohexyl methacrylate, MA: methyl acrylate, S: styrene, BA: butyl acrylate). These copolymers display varying compositional heterogeneity depending on the different radical reactivity ratios and the feed composition used. Curves of instantaneous copolymer composition versus fractional conversion and the distribution functions of chemical composition were calculated for the various systems. In addition, miscibility diagrams of corresponding low conversion copolymers AxB1-x and AyB1-y, derived from the same monomer pair (A, B) but differing in composition, were recorded at high temperatures. Phase separation was detected by light microscopy and differential scanning calorimetry (DSC) using cast films. The onset of phase separation depending on the actual stage of copolymerization was recorded. The composition of the copolymers at the onset of phase separation was compared with the miscibility of low conversion copolymer blends. A satisfactory prediction of the start of phase separation during copolymerization is presented.
    Additional Material: 6 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    Einsatz von Membrantrennverfahren in der chemischen IndustrieVortrag anläßlich der GVC-Jahrestagung, 24./26. Sept. 1997 in Dresden. (1998)
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 70 (1998), S. 1265-1270 
    Details
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Membran Processes in the Chemical Industry In the chemical industry (less so in the pharmaceutical industry), membrane technology is still at an early stage of acceptance. However, in this field too, it is gaining increasing importance as an innovative, reliable and, first and foremost, cost-effective alternative to conventional processes. This trend is evidenced by a variety of new plants which have been installed in recent years, and also by the rapidly growing interest in this technology shown by production and design departments. This article gives an overview of the industrial importance of membrane processes in chemical and pharmaceutical production and also presents some typical examples of applications in this field by describing plants which have already been installed and are now in production.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cite.330701005
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