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  • empirical equations  (2)
  • Hypophyseal neural lobe (pars nervosa)  (1)
  • LTR  (1)
  • Springer  (4)
  • American Physical Society
  • 2015-2019
  • 1995-1999  (4)
  • 1998  (4)
Collection
Publisher
  • Springer  (4)
  • American Physical Society
Years
  • 2015-2019
  • 1995-1999  (4)
Year
  • 1998  (4)
  • 1
    ISSN: 1432-1211
    Keywords: Key words HLA ; MHC ; DRB6 ; LTR ; Retrovirus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  HLA-DRB6 is one of the human major histocompatibility complex (MHC) genes present in DR1, DR2, and DR10 haplotypes (approximately 26% of individuals). It shows several anomalies in human and non-human primates, including exon 2 stop codons (non-randomly grouped between codons 74 and 94) and a promoter region, and an exon 1 coming from an inserted retrovirus. It has been shown that not only chimpanzee but also human Mhc-DRB6 lack the usual 3’ untranslated (UT) polyadenylation signal, and in the present work it was found that the human DRB6 gene coming from an HLA-DR2 haplotype is effectively transcribed after transfection in mouse L cells, and that HLA-DRB6 molecules may be expressed on the cell surface. DRB6 transcription level is remarkably lower in human than in chimpanzee. Moreover, their exons 1 (both taken from the 3’LTR region of a mammary tumor retrovirus) are also different; this shows that these viral insertions may be an important mechanism for different evolutionary changes in orthologous genes of different species. The pathways by which DRB6 molecules may be expressed on the membrane are unclear but other examples of truncated protein expression have also been described, even within the human major histocompatibility complex (i. e., in HLA-G). Finally, the presence of mature HLA-DRB6 mRNA molecules supports the notion that splicing may take place even in the absence of a canonical 3’UT polyadenylation signal.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1572-8943
    Keywords: empirical equations ; excess enthalpy ; group contribution ; ternary mixtures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Studies were previously made of the excess molar enthalpies, Hm E, at 298.15 K and normal atmospheric pressure of the ternary mixtures (ethyl propanoate or 3-pentanone) + (n-hexane) + (n-decane, n-dodecane or n-tetradecane) and (1-propanol) + (3-pentanone) + (n-hexane, n-heptane, n-octane, 1-hexanol or 1-heptanol). In the present work, experimental values of Hm E were correlated through use of the Cibulka and Nagata equations, and a new correlation equation is proposed. Experimental data were compared with the predictions of the UNIFAC I (version of Tassios), UNIFAC II (version of Larsen) and Nitta-Chao group contribution models. Hm E values were used to test several empirical predictive methods, both symmetric (Kohler, Jacob-Fitzner, Colinet and Knobeloch-Schwartz), and asymmetric (Tsao-Smith, Toop, Scatchard, Hillert and Mathieson-Tynne).
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  • 3
    ISSN: 1572-8943
    Keywords: butyl butanoate ; empirical equations ; excess volume ; Nitta-Chao model ; ternary mixture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Excess molar volumes at 298.15 K of the ternary system {CH3(CH2)2CO2(CH2)3CH3+ CH3(CH2)7OH+CH3(CH2)6CH3} and the binary mixtures {CH3(CH2)2CO2(CH2)3CH3+ CH3(CH2)7OH}, {CH3(CH2)2CO2(CH2)3CH3+CH3 (CH2)6CH3} and {CH3(CH2)7OH+ CH3(CH2)6CH3} were determined using an Anton Paar DMA 60/602 densimeter. All experimental values were compared with the results obtained with empirical expressions for estimation of the ternary properties from the binary data. Variable-degree polynomials were fitted to the results.
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  • 4
    ISSN: 1432-0878
    Keywords: Key words Gonadotropin-releasing hormone ; Brain ; Hypophyseal neural lobe (pars nervosa) ; Reproduction ; Bufo arenarum (Anura)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The presence and distribution of gonadotropin-releasing hormone (GnRH) in sexually mature specimens of Bufo arenarum was studied by reverse phase/high performance liquid chromatography (RP-HPLC) combined with radioimmunoassay and immunocytochemistry. The analysis of brain extracts with RP-HPLC followed by radioimmunoassay with PBL#45 antiserum showed the presence of only one peak with immunoreactivity for GnRH (ir-GnRH) having the chromatographic and immunological characteristics of mammalian GnRH (mGnRH). This peak was further analyzed with two mGnRH-specific antisera, EL-15 and m1076, yielding serial dilution displacement curves parallel to those obtained with the mGnRH synthetic standard. Immunocytochemical results with the monoclonal antibody LRH13 showed the presence of a terminal nerve-septo-preoptic system with neurons and fibers distributed from the olfactory bulb, septal area, and anterior preoptic area toward the hypothalamus and hypophyseal neural lobe. The main group of ir-GnRH fibers and neurons was identified in the anterior preoptic area. These neurons appear to be the origin of fibers that, after surrounding the preoptic recess, border the dorsal surface of the optic chiasma, extend through the infundibulum, traverse the external layer of the median eminence, and end in the pars nervosa.
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