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  • Chemistry  (6)
  • Cell & Developmental Biology  (1)
  • Polymer and Materials Science
  • 1995-1999  (7)
  • 1997  (7)
  • 1
    Electronic Resource
    Electronic Resource
    Differential stability of proteolytically active and inactive recombinant metalloproteinase in Chinese hamster ovary cells (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 53 (1997), S. 594-600 
    Details
    ISSN: 0006-3592
    Keywords: CHO ; mammalian cells ; metalloproteinase ; recombinant protein production ; expression stability ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Stability of heterologous protein expression during production is critical for regulatory approval of vaccine and therapeutic products. Leishmania GP63, a zinc metalloproteinase that is a potential vaccine candidate, has been expressed on the surface of Chinese hamster ovary (CHO) cells. Flow cytometry was used to follow the stability of GP63 expression. Expression of proteolytically active GP63 (GP63WT) was unstable whether or not methotrexate (MTX) selection was maintained. In contrast, expression of an active site mutant (GP63E265D) was stable under MTX selection. In the absence of selection, the decline in GP63E265D expression was more gradual than the loss of GP63WT expression. Different molecular mechanisms accounted for these losses and resulted in higher growth rate nonproducer populations. A dynamic population model was used to calculate the conversion rates of GP63WT producers to nonproducers. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 53: 594-600, 1997.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    1α,25-Dihydroxyvitamin D3 receptor as a mediator of transrepression of retinoid signaling (1997)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 67 (1997), S. 287-296 
    Details
    ISSN: 0730-2312
    Keywords: vitamin D3 receptor ; regulation of transcription ; retinoid signaling transrepression ; tumor necrosis factor-α receptor type I ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The receptors for retinoic acid (RA) and for 1α,25-dihydroxyvitamin D3 (VD), RAR, RXR, and VDR are ligand-inducible members of the nuclear receptor superfamily. These receptors mediate their regulatory effects by binding as dimeric complexes to response elements located in regulatory regions of hormone target genes. Sequence scanning of the tumor necrosis factor-α type I receptor (TNFαRI) gene identified a 3′ enhancer region composed of two directly repeated hexameric core motifs spaced by 2 nucleotides (DR2). On this novel DR2-type sequence, but not on a DR5-type RA response element, VD was shown to act through its receptor, the vitamin D receptor (VDR), as a repressor of retinoid signalling. The repression appears to be mediated by competitive protein-protein interactions between VDR, RAR, RXR, and possibly their cofactors. This VDR-mediated transrepression of retinoid signaling suggests a novel mechanism for the complex regulatory interaction between retinoids and VD. J. Cell. Biochem. 67:287-296, 1997. © 1997 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    Simulation von Reaktoren zur Emulsionspolymerisation (1997)
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 69 (1997), S. 1240-1241 
    Details
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cite.330690937
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  • 4
    Electronic Resource
    Electronic Resource
    First Results from the ESCA Microscopy Beamline on ELETTRA (1997)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 25 (1997), S. 374-379 
    Details
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: The results of the first experiments with test samples, tool steel and polycrystalline Sn carried out with the scanning photoemission microscope built on the ELETTRA storage ring in Trieste are presented and discussed. The scanning photoemission microscope uses a zone plate optical system for demagnification of the photon beam to submicron dimensions. The present performance of the microscope in photoemission allows elemental and chemical mapping and small-spot photoelectron spectroscopy with lateral resolution better than 200 nm. The reported results show how photoemission microscopy can distinguish the spatial distribution of carbide phases with different Fe content at the surface of a tool steel and the correlation between inhomogeneous oxidation of a polycrystalline Sn sample and its grain structure. © 1977 John Wiley & Sons, Ltd.
    Additional Material: 6 Ill.
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  • 5
    Electronic Resource
    Electronic Resource
    Determination of monomethylmercury cation in sediments by vacuum distillation followed by hydride derivatization and atomic fluorescence spectrometric detection (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Applied Organometallic Chemistry 11 (1997), S. 761-769 
    Details
    ISSN: 0268-2605
    Keywords: methylmercury ; dimethylmercury ; mercury speciation ; estuarine sediment ; sodium borohydride derivatization ; atomic fluorescence spectrometry ; Chemistry ; Industrial Chemistry and Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: This paper presents a method capable of quantitatively separating sub-nanogram amounts of monomethylmercury cation (MeHg+) and dimethylmercury from sediments by vacuum distillation at 40 °C and 6 μm Hg pressure followed by hydride derivatization and atomic fluorescence spectrometric detection. Concentrations of MeHg+ in Great Bay Estuarine sediments ranged from 2.2 to 7.3 ng g-1 (dry weight) with a 4.7 ng g-1 average for samples taken over nine weeks of the 1996 summer. The RSD for replicate determinations of a homogenized estuarine sediment is typically less than 6%. The detection limit for the routine determinations on MeHg+ is 0.2 ng g-1 dry weight of sediment. We validated the method by determining MeHg+ concentration in reference sediment S-19, by confirming our method against an established extraction method, and by recovering 85% of 10 ng MeHgCl spiked into estuarine sediments. This paper also includes significant improvements in the hydride derivatization method for mercury compounds relative to previous work with respect to faster analysis time and lower detection limits. © 1997 John Wiley & Sons, Ltd.
    Additional Material: 3 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    NMR chemical shielding surface of N-Acetyl-N′-Methylalaninamide: A density functional study (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 18 (1997), S. 126-138 
    Details
    ISSN: 0192-8651
    Keywords: Chemistry ; Theoretical, Physical and Computational Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: The five energetically lowest minima on the potential energy surface of N-acetyl-N′-methylalaninamide were optimized at the Becke3LYP/DZd level of theory to compare these density functional theory results with the literature findings at restricted Hartree-Fock/3-21G. While the relative energies are very similar, the amide moiety is predicted to be much more flexible at Becke3LYP/DZd. As a consequence, the three minima that favor a nonplanar amide group differ by up to 14° in their φ and ψ values between the two levels. To compare the change in the density functional NMR chemical shifts with respect to φ and ψ with experimental results, Becke3LYP/DZd was employed to optimize a structure for N-acetyl-N′-methylalaninamide at each 30° interval on the (φ, ψ) surface in the regions that correspond to the α helix and the β-pleated sheet and at each 60° interval elsewhere. The corresponding NMR chemical shielding surface was computed with the density functional program deMon. The resultant NMR chemical shielding surfaces for N and Cβ are in good agreement with the experiment, while the change in the NMR chemical shielding of C′ and Cα cannot be described only in terms of φ and ψ. The chemical shifts for those atoms also depend on the nonplanarity of the amide moiety. We evaluated this dependence for N-methylacetamide as a model system. Estimates of the parameters derived from N-methyl-acetamide allowed the NMR-shielding surfaces of C′ and Cα to be corrected for the nonplanar nitrogen influence. Although the effect is less pronounced with lower level theoretical geometries, due to the smaller degree of pyramidalization of the amide nitrogen, the (φ, ψ) NMR chemical shielding surfaces will need to be corrected. The agreement with the experiment was much better for the corrected surface of C′ when the nitrogen in the α helix had a nonplanar environment. © 1997 by John Wiley & Sons, Inc.
    Additional Material: 11 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    Cellular electrophoretic mobility data: A first approach to a database (1997)
    Weinheim : Wiley-Blackwell
    Electrophoresis 18 (1997), S. 1109-1119 
    Details
    ISSN: 0173-0835
    Keywords: Cell electrophoresis ; Electrophoretic mobility ; Ionic strength ; Electric field ; Standard cell ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Cellular electrophoretic mobility values of 288 types of eucaryotic cells were collected from literature published worldwide by a series of authors during the past forty years and arranged in a list. This list contains well-known recent electrophoretic results and also data that cannot be found anymore with modern literature retrieval systems. It will be a valuable help for scientists trying to purify cell populations. In addition, it confirms the observation that most eucaryotic cells have very similar electrophoretic mobilities, ranging from 40% above to 50% below the electrophoretic mobility of human erythrocytes, and thus reinforces the suggestion that electrophoretic mobilities of eucaryotic cells are subjected to strong biological controls.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150180715
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