ISSN:
1040-0397
Keywords:
Bioelectronics
;
Biosenzors
;
Enzyme electrodes
;
Enzyme monolayers
;
Enzyme multilayers
;
Reconstituted enzymes
;
Electrically contacted enzyme electrodes
;
Bioelectrocatalysis
;
Amperometric biosensors
;
NAD(P)+-dependent enzyme electrodes
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Tailoring of electrically contacted enzyme electrodes provides the grounds for bioelectronic and biosensor systems. Redox-enzymes organized onto electrodes as monolayer assemblies, and chemically functionalized by redox-relay groups, yield electrically contacted enzyme electrodes exhibiting bioelectrocatalytic features. The sensitivity of the enzyme electrode can be enhanced, or tuned, by the organization of multilayer enzyme electrodes and the application of rough metal supports. Enzyme electrodes of extremely efficient electrical communication with the electrode are generated by the reconstitution of apo-flavoenzymes onto relay-FAD monolayers associated with electrodes. The reconstitution process results in an aligned enzyme on the surface, and its effective electrical contact with the electrode yields selective enzyme electrodes of unprecedented high current responses. Integrated electrodes consisting of relay-NAD(P)+-cofactor and enzyme units are generated by the reconstitution of NAD(P)+-dependent enzymes onto a relay-NAD(P)+ monolayer assembly followed by lateral crosslinking of the enzyme network.
Additional Material:
17 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140091302
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