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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 17 (1997), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effects of the addition of starch, glucose, sodium chloride, sodium citrate, monopotassium phosphate and disodium phosphate to the recovery medium on apparent heat resistance of Bacillus cereus spores (ATCC 4342, 7004 and 9818) were investigated. Sodium citrate, monopotassium and disodium phosphate at concentrations of 0.1% were effective inhibitory agents for heat injured B. cereus spores especially for strain 9818, although only monopotassium and disodium phosphate caused a significant reduction (p 〈 0.05) in D-values obtained for strain 9818. Sodium chloride also had a marked effect on the recovery of heat injured spores. Concentration as low as 0.5% caused a significant reduction in the recovery rates for strains 9818 and 7004. In all cases, increasing the salt levels from 0.5 to 4% resulted in a progressive decrease in spore recovery. D-values gradually decreased as the salt content increased, although the concentrations which produced statistically significant differences (p 〈 0.05) varied among strains. The addition of starch at 0.1% resulted in a significant increase in the counts for strains 9818 and 7004. In contrast, glucose (0.1%), did not significantly modify the counts obtained Neither of these compounds affected decimal reduction times. No statistical significance (p〉0.05) differences were detected among z-values for the spores of the three strains recovered in the presence of different additives assayed. z-Values ranged from 6.67 to 8.32, with a mean value of 7.56 ± 0.46C.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 116 (1997), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The androgenic response of five varieties and 10 F1 intervarietal hybrids of 6 × triticale was analysed. The overall response involved three independent phenomena: the production of embryoids, the capability of embryoids to regenerate plants, and the production of green plants. Each of these factors appeared to be controlled by independent genetic systems. From a practical point of view, the product of these three factors, the number of green haploid plants per anther (GPA), is the most reliable variable for the evaluation of genotypes. Positive heterotic effects on GPA were observed in the hybrids, which may indicate that GPA is controlled predominantly by additive genes. No correlation was found between the number of green haploid plants produced by hybrids and that yielded by the best parental line. Therefore, it is difficult to predict the yield of green haploid plants that can be produced by a hybrid simply from the number produced by its parents.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Sedimentology 44 (1997), S. 0 
    ISSN: 1365-3091
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: An active oolitic sand wave was monitored for a period of 37 days in order to address the relationship between the direction and strength of tidal currents and the resultant geometry, and amount and direction of migration of bedforms in carbonate sands. The study area is situated in a tidal channel near Lee Stocking Island (Exumas, Bahamas) containing an estimated 5.5 to 6 × 105 m3 of mobile oolitic sand. Tidal ranges within the inlet are microtidal and the maximum current velocity at the studied site is 0.6 m s−1. At least 300–400 m3 of mostly oolitic sand are formed within, or brought into, the channel area every year. The tidal inlet is subdivided into an ocean-orientated segment, in which sand waves are shaped by both flood and ebb tides, and a platform-orientated segment, where sand waves are mainly shaped by flood tides. The studied sand wave lies on the platformward flood-tide dominated segment in a water depth of 3.5.4.5 m. During the 37 days of observation, the oolitic and bioclastic sand wave migrated 4 m in the direction of the dominant flood current. The increments of migration were directly related to the strength of the tide.During each tidal cycle, bedforms formed depending on the strength of the tidal current, tidal range and their location on the sand wave. During flood tides, a steep lee and a gentle stoss side formed and current ripples and small dunes developed on the crest of the sand wave, while the trough developed only ripples. The average lee slope of the sand wave is 24.2°, and therefore steeper than typical siliciclastic sand waves. During ebb tides, portions of the crest are eroded creating a convex upward ebb stoss side, covered with climbing cuspate and linguoid ripples and composite dunes. The area between the ebb-lee side and the trough is covered with fan systems, sinuous ripples and dunes.The migration of all bedforms deviated to a variable degree from the main current direction, reflecting complex flow patterns in the tidal inlet. Small bedforms displayed the largest deviation, migrating at an angle of up to 90° and more to the dominant current direction during spring tides.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 62 (1997), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Physical and chemical characteristics were evaluated on brown rot and phomopsis decayed tissue in peaches, to investigate its removal by washing with high pressure water sprays and to evaluate quality of puree made after washing. Washing treatments were at 345 to 758 kPa for up to 10, 1-s exposures. Results indicated brown rot tissue was darker, less yellow and had more soluble solids than the softer phomopsis decayed tissue. Nearly 100% of phomopsis decayed and 70% of brown rot tissue were removed with high pressure water treatment. Removal improved the quality of peach puree, but medium/large lesions were more detrimental to quality than small lesions.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 99 (1997), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A calcium-regulated protein kinase (CRPK) associated with the plasma membrane of beet root cells was purified by deoxycholate extraction of plasma membrane proteins followed by chromatography in DEAE- and phenyl-Sepharose columns. This procedure rendered a purified CRPK preparation with a specific activity higher than 2.2 μmol mg-1min-1 using histone III-S as substrate. The molecular mass of CRPK, estimated by SDS-PAGE, was 58 kDa. Gel filtration and glycerol gradient centrifugation indicated that the native enzyme is a monomer. Phosphorylation of histone by CRPK displayed a broad pH optimum between 7.0 and 8.2, Syntide-2, a CaM-kinase II substrate, was phosphorylated much more efficiently than kemptide, Ac-MBP (4–14), CKII substrate or poly-(Glu, Tyr) 4:1. The kinetics of this enzyme can be described by a steady-state ordered mechanism in which four bound Ca2+ ions are required for the phosphorylation of the peptide substrate. After Ca2+ binding, the affinity of the enzyme for ATP was not affected while that for syntide-2 or histone decreased. Curves of kinase activity vs ATP at different syntide-2 concentrations indicated that the Km values for ATP or syntide-2 increased with increasing cosubstrate concentrations. Similar curves of kinase activity vs ATP at different histone concentrations indicated that the apparent affinities of CRPK for ATP or histone were not affected by the cosubstrate concentration. The purified CRPK was not recognized by antibodies against soybean or Arabidopsis calcium-dependent protein kinase (CDPK). These results suggest that CRPK and CDPKs could be different enzymes, although they show some similar properties.
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  • 6
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A PCR-based strategy with two degenerate oligonucleotides followed by library screening was used to isolate a 590-bp cDNA clone encoding a full-length apocytochrome c polypeptide from a sunflower (Helianthus annuus L. line HA401B) root library. The sequence of the encoded polypeptide (112 amino acids) differs from the previously reported protein sequence (Ramshaw et al. 1970. Biochem. J. 119: 535-539) at four positions. Southern blot analysis indicates the possible existence of two different cytochrome c genes in sunflower. The isolated cDNA clone was used to perform expression studies of cytochrome c mRNA(s) in different sunflower organs and under different conditions. Higher transcript levels were observed in flowers and roots relative to leaves, stems, hypocotyls and cotyledons. Light promoted an increase in the expression of cytochrome c mRNA in 6-day-old seedlings. Nitrate treatment, in tum, reduced expression in roots. It is concluded that the steady-state mRNA levels of cytochrome c encoding transcripts in sunflower are subject to tissue-specific regulation and also respond to environmental factors.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Fatigue & fracture of engineering materials & structures 20 (1997), S. 0 
    ISSN: 1460-2695
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract— Since the degradation effect due to environment on the cracking of materials depends on time, the loading rate has an important influence on the parameters that characterise its behaviour. This work analyses the effect of loading rate on the resistance to hydrogen induced cracking (HIC) of two microailoyed steels, E690 and E500. Monotonic loading tests were performed on precracked CT samples using a slow strain rate machine. Tests were done under constant displacement rate varying from 4.1 × 10–7 m/s to 8.2 × 10–10m/s on the two steels that were cathodically charged with hydrogen at different current densities (1, 5 and 10 mA/cm2) to obtain different hydrogen concentration levels inside the material.Based on an analytical study, the initiation conditions for cracking as well as the crack propagation rates were determined in each case, and analysed as a function of K1. An extensive fractographic SEM study has been performed to help in the analysis of the different zones of behaviour obtained as an effect of loading rate, for each material and environmental condition used.
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  • 8
    ISSN: 1540-5915
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Economics
    Notes: This paper develops the idea of animation in user interfaces designed for decision support systems (DSS), proposes a framework to investigate the efficacy of animation in these interfaces, and reports on a study that examined the effects of properties of animation specified by the framework. Based on a review of selected background literature, principal properties affecting the efficacy of animation in user interfaces designed for DSS are identified and the effects on decision quality of three of these properties are hypothesized. To evaluate these hypotheses, data was collected in a laboratory experiment involving two different tasks. The results for both tasks indicate that animation in user interfaces designed for DSS should employ parallel as opposed to sequential navigation interactivity techniques. The decision quality of subjects that used a parallel navigation technique was significantly greater than that of those that used a sequential navigation interactivity technique. The results regarding the efficacy of image abstraction and transition effects varied by task. For one task, decision quality was significantly greater for subjects that used realistic as opposed to abstract images, but decision quality did not vary by transition effect. For the other task, decision quality was significantly greater for subjects that used gradual as compared to abrupt transition, but image abstraction had no effect on decision quality.
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS immunology and medical microbiology 19 (1997), S. 0 
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: In Spain, in November 1995, an epidemiological alert recommended the surveillance of Salmonella serogroup G. The nine clinical isolates collected after and the four collected before the alert in Asturias were differentiated into six clonal lines by the combination of results from HincII ribotyping, PCR ribotyping, and RAPD typing using primers named A and S. The seven Gumpensis isolates showed identical DNA fingerprinting with the four typing procedures falling into a line. Six of these were collected during May–August from people living in a single health area suggesting that they could be associated with a community outbreak. The four Worthington isolates fell into three other lines, one Poona isolate into another line and one Havana isolate into another. 100% typeability was shown with all methods. The reproducibility of HincII ribotyping was better than that of PCR-based methods, although these were less time-consuming. The highest discriminatory power was obtained with HincII ribotyping and RAPD typing using primer A.
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 151 (1997), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Non-growing cells of Escherichia coli O157:H7 and K-12 that were incubated anaerobically in sodium phosphate buffer at pH 6.5 consumed glucose at a rate of approximately 8 μmol·(mg protein)−1·h−1 and had intracellular pH values of 7.3 and 7.5, respectively. The uncoupler, carbonylcyanide-m-chlorophenylhydrazone (CCCP), caused a marked decrease in intracellular pH, ATP and potassium of both strains. Low concentrations of CCCP stimulated glucose consumption rate, but higher concentrations were inhibitory. Acetate also caused a decrease in intracellular pH, but it never caused a large decrease in glucose consumption rate. Acetate decreased the intracellular ATP of E. coli K-12, but it had no effect on the ATP of O157:H7. Acetate had no effect on the intracellular potassium of E. coli O157:H7, and acetate-treated K-12 cells had even more potassium than untreated controls. Based on these results, acetate and CCCP appear to have different effects on E. coli. The comparison of E. coli O157:H7 and K-12 indicated that intracellular pH, acetate accumulation and intracellular potassium were related. E. coli K-12 maintained a higher intracellular pH than O157:H7, accumulated more acetate and had a greater intracellular potassium.
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