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  • RFLP  (7)
  • Springer  (7)
  • American Association of Petroleum Geologists
  • Frontiers Media SA
  • Institute of Physics
  • Wiley
  • 2020-2024
  • 2015-2019
  • 2010-2014
  • 1995-1999  (7)
  • 1975-1979
  • 1960-1964
  • 1920-1924
  • 1997  (7)
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Keywords
Publisher
  • Springer  (7)
  • American Association of Petroleum Geologists
  • Frontiers Media SA
  • Institute of Physics
  • Wiley
Years
  • 2020-2024
  • 2015-2019
  • 2010-2014
  • 1995-1999  (7)
  • 1975-1979
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Year
  • 1
    Electronic Resource
    Electronic Resource
    QTLs associated with chlorimuron ethyl sensitivity in soybean: Effects on seed yield and related traits (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 971-974 
    Details
    ISSN: 1432-2242
    Keywords: Key words Soybean ; Glycine max ; QTLs ; RFLP ; Chlorimuron ethyl ; Seed yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Soybean, Glycine max (L.) Merr., genotypes are known to differ in chlorimuron ethyl sensitivity (CS). Earlier we have reported two putatively independent marker loci linked to two quantitative trait loci (QTLs) controlling CS in a soybean population derived from a cross of PI97100 (sensitive to chlorimuron ethyl) and ‘Coker 237’ (tolerant to chlorimuron ethyl). The objective of the present study was to quantify the association of the two marker loci with seed yield and related traits in this soybean population following application of chlorimuron ethyl. Phenotypic data were collected for 111 F2-derived lines of the cross grown in replicated plots at Athens, G.A., in 1994 and 1995, and at Blackville, S.C., in 1995. The two CS marker loci explained as much as 50% of the genetic variation in seed yield and seed number m-2, but had no association with seed weight, plant height, lodging, seed protein, and seed oil. There were no epistatic interactions between the two marker loci for any of the traits. The marker locus (cr168-1 on USDA linkage group E) linked to the major CS QTL explained between 13 and 23% of the variation in seed yield. The Coker 237 allele at this locus was associated with decreased CS and increased seed yield. The marker locus (Blt015-2 on an unknown linkage group) linked to the minor CS QTL accounted for a maximum of 11% of the variation in seed yield. The Coker 237 allele at this locus was associated with an increase in CS and a decrease in seed yield. The association of the two marker loci with seed number m-2 strongly resembled their association with seed yield. Seed yield had a strong positive correlation (r=0.74 – 0.94) with seed number m-2, and the effect of chlorimuron ethyl on seed yield was due mainly to its effect on seed number m-2 rather than seed weight.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050503
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  • 2
    Electronic Resource
    Electronic Resource
    Conservation of S-locus for self-incompatibility in Brassica napus (L.) and Brassica oleracea (L.) (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 73-82 
    Details
    ISSN: 1432-2242
    Keywords: Key words Self-incompatibility ; Brassica oleracea ; Brassica napus ; RFLP ; PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Self-incompatibility (SI) in Brassica is a sporophytic system, genetically determined by alleles at the S-locus, which prevents self-fertilization and encourages outbreeding. This system occurs naturally in diploid Brassica species but is introduced into amphidiploid Brassica species by interspecific breeding, so that in both cases there is a potential for yield increase due to heterosis and the combination of desirable characteristics from both parental lines. Using a polymerase chain reaction (PCR) based analysis specific for the alleles of the SLG (S-locus glycoprotein gene) located on the S-locus, we genetically mapped the S-locus of B. oleracea for SI using a F2 population from a cross between a rapid-cycling B. oleracea line (CrGC-85) and a cabbage line (86-16-5). The linkage map contained both RFLP (restriction fragment length polymorphism) and RAPD (random amplified polymorphic DNA) markers. Similarly, the S-loci were mapped in B. napus using two different crosses (91-SN-5263×87-DHS-002; 90-DHW-1855-4×87-DHS-002) where the common male parent was self-compatible, while the S-alleles introgressed in the two different SI female parents had not been characterized. The linkage group with the S-locus in B. oleracea showed remarkable homology to the corresponding linkage group in B. napus except that in the latter there was an additional locus present, which might have been introgressed from B. rapa. The S-allele in the rapid-cycling Brassica was identified as the S29 allele, the S-allele of the cabbage was the S 5 allele. These same alleles were present in our two B. napus SI lines, but there was evidence that it might not be the active or major SI allele that caused self-incompatibility in these two B. napus crosses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050534
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  • 3
    Electronic Resource
    Electronic Resource
    Relationship between seed yield heterosis and molecular marker heterozygosity in soybean (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 460-467 
    Details
    ISSN: 1432-2242
    Keywords: Key words Glycine max ; RFLP ; Isozyme ; Geographic origin ; Hybrid vigor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In soybean [Glycine max (L.) Merr.] heterosis has been reported for seed yield. Molecular markers may be useful to select diverse parents for the expression of heterosis and yield improvement. The objective of this study was to determine if molecular markers could be used to predict yield heterosis in soybean. From each Maturity Group (MG) II and III, 21 genotypes were selected on the basis of high yield (HY), different geographic origin (GO), and isozyme loci (ISO) and for diversity in restriction fragment length polymorphisms (RFLP), and crosses were made within MGs and selection criteria groups to obtain 6 F1 hybrids per group. The 21 parents and the 24 F1 hybrids of each MG were evaluated for yield in replicated tests at two locations in 2 years, and midparent heterosis (MPH) and high-parent heterosis (HPH) estimates were calculated. On the basis of hybrid performance during the first year, 12 parents (3 per selection criteria group) were chosen in each MG to conduct a second RFLP analysis using 129 probes. Genetic distances (GDM) for pairs of the 12 genotypes were calculated with this RFLP information and correlated with MPH and HPH estimates. Significant MPH averages for seed yield were observed in the combined analysis of variance in each of the four selection criteria groups of MG II, and in the HY, ISO, and GO of MG III. Significant HPH averages were observed only in the ISO and GO groups of MG II. The greatest frequency of F1 hybrids with significant MPH was observed in the ISO and GO groups of both MGs. For HPH, the greatest frequency was observed in the ISO group of both MGs. In both MGs, the ISO group had the largest absolute MPH value; the RFLP group had generally the smallest. The observations indicated that the expression of heterosis in seed yield might be associated with diversity in the isozyme loci present in the parents. For the genotypes included in the second RFLP analysis, correlations of GDMs with MPH and HPH values on an entry-mean basis were low and not significant, indicating that heterosis in yield may not be associated with genetic diversity at the molecular level as determined by RFLPs. The results suggest that in soybean, parent selection on the basis of RFLPs and isozyme loci to exploit heterosis in seed yield may not be feasible. There was no association between genetic distance estimated by the RFLP analysis and seed yield heterosis, and in spite of the observed relationship between isozyme loci and heterosis for yield, the practicality of using the isozyme markers to select parents may be limited because of the reduced number of assayable isozyme loci in soybean.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050583
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  • 4
    Electronic Resource
    Electronic Resource
    Evaluation of the extent of genetic variability among Theobroma cacao accessions using RAPD and RFLP markers (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 10-19 
    Details
    ISSN: 1432-2242
    Keywords: Key words Theobroma cacao ; RFLP ; RAPD ; Genetic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) markers were used to evaluate genetic relationships within the Theobroma cacao species and to assess the organization of its genetic diversity. Genetic variability was estimated with 18 primers and 43 RFLP probes on 155 cocoa trees belonging to different morphological groups and coming from various geographic origins. The majority of the RFLP probes issued from low-copy DNA sequences. On the basis of on the genetic distance matrices, the two molecular methods gave related estimates of the genetic relationship between genotypes. Although an influence of cocoa morphological groups and geographical origins of trees was observed, a lack of gene differentiation characterized the T. cacao accessions studied. The continuous RFLP variability observed within the species may reflect the hybridization and introgressions between trees of different origins. Nevertheless, the Nacional type was detected to be genetically specific and different from well-known types such as Forastero, Criollo and Trinitario. Some of those genotypes were characterized by a low heterozygosity rate and may constitute the original Nacional pool. These results also provide information for the constitution of a cocoa tree core collection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050527
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  • 5
    Electronic Resource
    Electronic Resource
    The genetics of resistance to five races of downy mildew (Plasmopara halstedii) in sunflower (Helianthus annuus L.) (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 584-589 
    Details
    ISSN: 1432-2242
    Keywords: Key words Linkage ; Major gene ; Race-specific resistance ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  These studies were undertaken to determine whether downy mildew resistance genes in sunflower were independent as first reported, or linked as suggested by more recent hypotheses. The segregations for downy mildew reaction of 111 F3 progenies from a cross between a susceptible line and a line with Pl2 were used to locate this gene on the sunflower consensus RFLP linkage map. It was shown that Pl2 was linked to the same RFLP markers on linkage group 1 as Pl1 and Pl6, mapped earlier, and at a very similar distance. The F3 progenies showed exactly the same segregation patterns when tested with race 1 and race D. One hundred and fifty four progenies from a cross between a susceptible line and HA335, containing Pl6 (considered as giving resistance to all Plasmopara halstedii races), were tested with the five French downy mildew races, 1, A, B, C and D. Two progenies were observed to show segregation for races 1 and D, while appearing homozygous-resistant to races A , B and C. Tests on F4 progenies confirmed this separation of resistances with fixation of susceptibility to races 1 and D and resistance to races A, B and C. It is concluded that the Pl6 gene is not a “strong” gene, giving resistance to all downy mildew races, but rather a cluster of genes, each providing resistance to one, or a few, downy mildew races. The genes giving resistance to races 1 and D, on one hand, and to races A, B and C, on the other hand, must be very closely linked, with about 0.6 cM between the two groups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050599
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  • 6
    Electronic Resource
    Electronic Resource
    Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 714-722 
    Details
    ISSN: 1432-2242
    Keywords: Key words Barley ; Genetic relationships ; Molecular analysis ; RFLP ; AFLP ; RAPD ; SSR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  RFLPs, AFLPs, RAPDs and SSRs were used to determine the genetic relationships among 18 cultivated barley accessions and the results compared to pedigree relationships where these were available. All of the approaches were able to uniquely fingerprint each of the accessions. The four assays differed in the amount of polymorphism detected. For example, all 13 SSR primers were polymorphic, with an average of 5.7 alleles per primer set, while nearly 54% of the fragments generated using AFLPs were monomorphic. The highest diversity index was observed for AFLPs (0.937) and the lowest for RFLP (0.322). Principal co-ordinate analysis (PCoA) clearly separated the spring types from the winter types using RFLP and AFLP data with the two-row winter types forming an intermediate group. Only a small group of spring types clustered together using SSR data with the two-row and six-row winter varieties more widely dispersed. Direct comparisons between genetic similarity (GS) estimates revealed by each of the assays were measured by a number of approaches. Spearman rank correlation ranked over 70% of the pairwise comparisons between AFLPs and RFLPs in the same order. SSRs had the lowest values when compared to the other three assays. These results are discussed in terms of the choice of appropriate technology for different aspects of germplasm evaluation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050617
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  • 7
    Electronic Resource
    Electronic Resource
    High-resolution genetical and physical mapping of the Rx gene for extreme resistance to potato virus X in tetraploid potato (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 153-162 
    Details
    ISSN: 1432-2242
    Keywords: Key words PVX ; Resistance gene ; Mapping ; RFLP ; AFLP ; CAPS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The Rx locus in potato confers extreme resistance to PVX. In the F1 progeny of crosses between the PVX-susceptible cultivar Huinkel and the cultivar Cara (Rx genotype) there was a 1 : 1 segregation of PVX resistance, indicating that Rx in Cara is present in the simplex condition. Using potato and tomato RFLP markers, we mapped Rx in Cara to the distal end of chromosome XII at a different position to the previously mapped Rx1 locus. To generate a high-resolution linkage map in the vicinity of Rx a total 728 AFLP primer combinations were screened using DNA of bulked resistant and susceptible segregants. We also screened segregating populations for chromosomal recombination events linked to the Rx locus and identified 82 plants with recombination events close to Rx. Using these recombinant plants we have identified AFLPs that flank Rx and span an interval of 0.23 cM in a region of the genome where 1 cM corresponds to approximately 400 kb.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050543
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