ISSN:
1573-5028
Keywords:
antifungal protein
;
Castanea sativa
;
chitinase
;
seed defence protein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Ch3, an endochitinase of 32 kDa present in Castanea sativa cotyledons, showed in vitro antifungal properties when assayed against Trichoderma viride. The characterization of a cDNA clone corresponding to this protein indicated that Ch3 is a class Ib endochitinase that is synthesized as a preprotein with a signal sequence preceding the mature polypeptide. Bacterial expression of mature Ch3 fused to the leader peptide of the periplasmic protein ompT resulted in active Ch3 enzyme. A plate assay was adapted for semi-quantitative determination of chitinase activity secreted from cultured bacteria, which should facilitate the identification of mutants with altered capacity to hydrolyse chitin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00041402
Permalink
