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  • Column liquid chromatography  (3)
  • RFLP  (2)
  • Springer  (5)
  • International Union of Crystallography (IUCr)
  • 1995-1999  (5)
  • 1996  (5)
Collection
Keywords
Publisher
  • Springer  (5)
  • International Union of Crystallography (IUCr)
Years
  • 1995-1999  (5)
Year
  • 1996  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Non-Mendelian and skewed segregation of DNA markers in wide crosses of the bean rust fungus,Uromyces appendiculatus (1996)
    Springer
    Current genetics 29 (1996), S. 159-167 
    Details
    ISSN: 1432-0983
    Keywords: Genetic linkage mapping ; Segregation distortion ; RAPD ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The inheritance of DNA markers was investigated in 27 F2 progeny from a single F1 hybrid derived from a wide cross inUromyces appendiculatus. This cross was unusual because asexual spores were used to fertilize sexual fruiting structures. Sixty percent of the DNA markers failed to segregate according to simple Mendelian ratios. Segregation bias was evident, in that F2 progeny inherited on average 91 % of maternal bands and 52% of paternal bands, which deviates significantly from the expected value for each of 75% for dominant markers. Because of these distortions, linkage mapping was not possible with this population. Evaluation of two F1s from a second wide cross, reciprocals obtained by normal fertilization, also showed non-Mendelian inheritance of one of three co-dominant RFLPs and five of six isozyme markers, indicating that the method of crossing was probably not responsible for the abnormal segregation patterns in the first cross. Either genetic incompatibility, similar to that of an interspecific cross, or selection of particular genotypes could explain the genetic anomalies reported here.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02221580
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Non-Mendelian and skewed segregation of DNA markers in wide crosses of the bean rust fungus, Uromyces appendiculatus (1996)
    Springer
    Current genetics 29 (1996), S. 159-167 
    Details
    ISSN: 1432-0983
    Keywords: Key words Genetic linkage mapping ; Segregation distortion ; RAPD ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The inheritance of DNA markers was investigated in 27 F2 progeny from a single F1 hybrid derived from a wide cross in Uromyces appendiculatus. This cross was unusual because asexual spores were used to fertilize sexual fruiting structures. Sixty percent of the DNA markers failed to segregate according to simple Mendelian ratios. Segregation bias was evident, in that F2 progeny inherited on average 91% of maternal bands and 52% of paternal bands, which deviates significantly from the expected value for each of 75% for dominant markers. Because of these distortions, linkage mapping was not possible with this population. Evaluation of two F1s from a second wide cross, reciprocals obtained by normal fertilization, also showed non-Mendelian inheritance of one of three co-dominant RFLPs and five of six isozyme markers, indicating that the method of crossing was probably not responsible for the abnormal segregation patterns in the first cross. Either genetic incompatibility, similar to that of an interspecific cross, or selection of particular genotypes could explain the genetic anomalies reported here.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050031
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  • 3
    Electronic Resource
    Electronic Resource
    Comparative study of the use of high-performance liquid chromatography and capillary electrophoresis for determination of phenolic compounds in water samples (1996)
    Springer
    Chromatographia 43 (1996), S. 619-624 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Capillary electrophoresis ; Phenolic compounds ; Water samples
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A comparative study of high-performance liquid chromatography (HPLC) and capillary zone electrophoresis (CZE) for determination of eleven EPA priority phenols in water samples is discussed. The repeatability, linearity and detection limits of both methods are compared. A solid-phase extraction method using a highly cross-linked styrene-divinylbenzene copolymer sorbent is used for the off-line preconcentration process in order to determine these compounds in water at low levels. Both methods are used to determine phenolic compounds in Ebro river and industrial waste water. Identification was achieved by comparison of the UV spectra corresponding to the different peaks, acquired with a diode-array detector, with those in a spectral library.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02292977
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  • 4
    Electronic Resource
    Electronic Resource
    Chromatographic determination of caffeine in pharmaceutical formulations using micellar mobile phases (1996)
    Springer
    Chromatographia 43 (1996), S. 149-152 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Caffeine ; Pharmaceutical formulations ; Micellar liquid chromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An HPLC procedure is described for the determination of caffeine in pharmaceutical preparations. A Spherisorb octadecylsilane ODS-2 C18 analytical column and spectrophotometric detection at 273 nm were used. The chromatographic behaviour of caffeine with different micellar eluents containing sodium dodecyl sulphate (SDS) is described. The determination of caffeine in pharmaceutical preparations was performed by use of a mobile phase containing 0.05 M sodium dodecylsulphate (SDS) and 1.5% propanol at pH7. At a 6.0 μg mL−1 concentration level the peak area and peak height repeatability were 2.6 and 2.4%, respectively. The application of the proposed method to the analysis of five pharmaceutical formulations, using peak heights as the dependent variable, gave recoveries between 85 and 104% of the values declared by the manufacturers. The proposed procedure for the determination of caffeine is rapid (15 min per sample), reliable and free from interferences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02292943
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  • 5
    Electronic Resource
    Electronic Resource
    HPLC determination of pesticides in green bean samples after SPE clean-up (1996)
    Springer
    Chromatographia 43 (1996), S. 265-270 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Diode array detection ; Solid-phase extraction (SPE) ; Pesticide analysis ; Multiresidue methods ; Green bean samples
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A reversed-phase high performance liquid chromatographic (HPLC) method with an acetonitrile-methanol-water mobile phase gradient and photodiode-array detection (DAD) is described for simultaneous determination of 21 pesticides, frequently used in agriculture, of different types, namely organophosphorus, organochlorine,N-methylcarbamates, triazines and phenylureas in vegetable samples. The pesticides were extracted with acetone and then partitioned from the vegetable sample with dichloromethane. Sample clean-up was accomplished by solid-phase extraction (SPE) using both C18 and florisil SPE columns. Average recoveries from green beans ranged from 70.0 to 110.1%. Detection limits of less than 0.1 mg kg−1 were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02270992
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