ISSN:
1432-203X
Keywords:
Abbreviations: NAA: 1-naphthaleneacetic acid; BA: benzyladenine; MS: Murashige and Skoog (1962); GUS: β-glucuronidase (EC 3.2.1.31); NPT II: neomycin phosphotransferase (EC 2.7.1.95); NOS: nopaline synthase; CaMV: Cauliflower Mosaic Virus; CTAB: cetyl triethylammonium bromide; X-GLUC: 5-bromo-4-chloro-3-indolyl-β-D-glucuronide; PCR: polymerase chain reaction
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract. We have developed an Agrobacterium-mediated transformation system for commercial Begonia species. The leaf explants of Begonia semperflorens, Begonia x hiemalis and B. tuberhybrida were inoculated with Agrobacterium tumefaciens LBA4404 harboring a binary vector pBI121 which contains rolA,B and C genes of an agropine type Ri plasmid (pRiA4b). Kanamycin resistant shoots of B. tuberhybrida were obtained on MS agar medium supplemented with 0.1 mg/l NAA, 0.5 mg/l BA, 500 mg/l claforan and 100 mg/l kanamycin. These shoots exhibited GUS activity and Southern analysis showed a single copy insertion into the genome. When the transgenic plants were transferred to soil, they displayed the phenotype specific to the transgenic plants by A. rhizogenes such as dwarfness, delay of flowering, and wrinkled leaves and petals.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050083
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