Publication Date:
1996-01-12
Description:
Early stages of T cell development are thought to include a series of coordinated interactions between thymocytes and other cells of the thymus. A monoclonal antibody specific for mouse CD81 was identified that blocked the appearance of alpha beta but not gamma delta T cells in fetal organ cultures initiated with day 14.5 thymus lobes. In reaggregation cultures with CD81-transfected fibroblasts, CD4-CD8- thymocytes differentiated into CD4+CD8+ T cells. Thus, interactions between immature thymocytes and stromal cells expressing CD81 are required and may be sufficient to induce early events associated with T cell development.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Boismenu, R -- Rhein, M -- Fischer, W H -- Havran, W L -- AI32751/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 1996 Jan 12;271(5246):198-200.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Immunology, Scripps Research Institute, La Jolla, CA 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8539618" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Antibodies, Monoclonal/immunology
;
Antigens, CD/genetics/immunology/*physiology
;
Antigens, CD4/analysis
;
Antigens, CD8/analysis
;
Antigens, CD81
;
Base Sequence
;
CHO Cells
;
Cell Differentiation
;
Cricetinae
;
Membrane Proteins/immunology/*physiology
;
Mice
;
Molecular Sequence Data
;
Organ Culture Techniques
;
Receptors, Antigen, T-Cell, alpha-beta/*analysis
;
Receptors, Antigen, T-Cell, gamma-delta/analysis
;
Stromal Cells/immunology
;
T-Lymphocyte Subsets/cytology/*immunology
;
Thymus Gland/cytology/*immunology
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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