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  • Medicago  (2)
  • 1995-1999  (2)
  • 1990-1994
  • 1996  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Cloning and characterization of PO22, a pollen-expressed gene in alfalfa (1996)
    Springer
    Plant molecular biology 32 (1996), S. 1205-1207 
    Details
    ISSN: 1573-5028
    Keywords: Medicago ; tissue-specific ; promoter ; microgametogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genomic and cDNA clones have been isolated for a new gene (PO22) expressed in mature pollen of alfalfa. PO22 represents a single-or low-copy gene containing 4 introns, 2 transcriptional start sites, and coding for a predicted protein of 340 amino acids with no signal peptide and a coiled structure of39 amino acids near the carboxyl end. The amino acid sequence of PO22 is 38% identical to that of an early nodule-specific gene (ENOD8), also from alfalfa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041407
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    PO149, a new member of pollen pectate lyase-like gene family from alfalfa (1996)
    Springer
    Plant molecular biology 32 (1996), S. 1037-1042 
    Details
    ISSN: 1573-5028
    Keywords: Medicago ; pollen-specific ; promoter ; microgametogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract PO149 is a low-copy-number gene expressed in the late stages of pollen development. The promoter region contains no similarities in DNA sequence to those of other pollen-specific genes, except for a tobacco sequence (AAATGA), which occurs four times in this alfalfa gene and much further upstream than in tobacco. Four distinct TATA boxes were detected in the promoter with the distal and proximal TATA boxes being separated by a spacer of 269 nucleotides. Hairpin loop structures were found in the 5′-and 3′-untranslated regions of PO149 mRNA. The coding region of PO149 is interrupted by two introns and encodes a putative prepeptide of 450 amino acids with homology to pollen pectate lyase-like proteins and pollen allergens. The coding region also contains sequences characteristic of both a signal peptide and a nuclear localization signal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041387
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    Paper (German National Licenses)
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