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  • 2000-2004
  • 1995-1999  (23)
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  • 1996  (23)
  • 1
    Electronic Resource
    Electronic Resource
    Bingley : Emerald
    International journal of health care quality assurance 9 (1996), S. 23-27 
    ISSN: 0952-6862
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Medicine , Economics
    Notes: Discusses some of the current problems associated with observational quality measurement. Uses Lerninger's conceptual theory-generating model as the basis of discussion. Puts forward a qualitative approach which breaks away from more traditional methods.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 384 (1996), S. 74-77 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Attention can be moved under voluntary or involuntary con-trol7'9. A technique developed to study experimentally the dynamics of visual attention involves presentation of a cue, which indicates the target position before onset of that target8. This improves accuracy of target detection and ...
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  • 3
    ISSN: 1432-2048
    Keywords: V-ATPase-immunocytochemistry ; Pea cotyledon ; Plasma membrane ; Putative γ-TIP ; Pyrophosphatase ; Tonoplast markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The tonoplast is usually characterized by the presence of two electrogenic proton pumps: a vacuolartype H+-ATPase and a pyrophosphatase, as well as a putative water-channel-forming protein (γ-TIP). Using a post-embedding immunogold labelling technique, we have detected the presence of these transport-protein complexes not only in the tonoplast, but also in the plasma membrane and trans Golgi elements of maturing pea (Pisum sativum L.) cotyledons. These ultrastructural observations are supported by Western blotting with highly purified plasma-membrane fractions. In contrast to the vacuolar-type H+-ATPase, whose activity was not measurable, considerable pyrophosphatase activity was detected in the plasma-membrane fraction. These results are discussed in terms of a possible temporary repository for tonoplast proteins en route to the vacuole.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Transgenic research 5 (1996), S. 359-362 
    ISSN: 1573-9368
    Keywords: virus resistant plants ; virus-derived inserts ; transcapsidation ; recombination ; synergism ; environmental risk assessment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sequences derived from the genomes of plant viruses are being used to provide virus resistance in transgenic crop plants. Although the environmental hazards associated with the release of such plants have been discussed widely, it has not been possible to reach generally acceptable conclusions about their safety. A case-by-case approach to the risk assessment of real examples is recommended as a means of building up confidence and of indicating areas of uncertainty. A logical framework for risk assessment is suggested, a key feature of which is identification of the viruses in the release environment that may infect the transgenic plants. Each of these is considered in relation to each of the three main classes of hazard (transcapsidation, recombination and synergism), and the risk associated with each event is analysed.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Vegetation history and archaeobotany 5 (1996), S. 1-11 
    ISSN: 1617-6278
    Keywords: Agrarian practices ; Granary ; Weeds ; Iron Age ; Denmark
    Source: Springer Online Journal Archives 1860-2000
    Topics: Archaeology , Biology
    Notes: Abstract In the Late Pre-Roman Iron Age an underground granary in the village of Overbyg»rd was destroyed by fire and the contents were carbonised. Almost 2000 years later, analyses of the macroremains of the granary, which included a range of processed and unprocessed crops and weed seeds, showed that naked barley (Hordeum vulgare var. nudum) and bread wheat (Triticum aestivum s.l.) were the main crops cultivated, hulled barley (Hordeum vulgare) and flax (Linum usitatissimum) also played a role, whereas emmer (Triticum dicoccum) and gold of pleasure (Camelina sativa) were present as weeds or contaminants. The arable weed flora suggests that crops were sown in spring and that ecological conditions in the arable fields were very variable. The crops were harvested on the straw and may have spent some time drying and maturing in the fields before being transported home to be stored as severed ears in pest-proof granaries. Winnowing or, more probably, casting appears to have been used to clean the crop after threshing. It could not be ascertained if the crops had been sieved. Large collections of weed seeds in the granary were apparently the result of intentional gathering for food, rather than by-products of crop processing. In the light of the investigation it is suggested that future research into Iron Age agrarian practices should include both the analysis of archaeobotanical finds and a programme of practical experiments. This dual approach will give us a much better understanding of arable agriculture, not only in the Iron Age, but in prehistory as a whole.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 187 (1996), S. 57-66 
    ISSN: 1573-5036
    Keywords: compensation ; growth ; heterogeneity ; inflow ; nutrient ; roots ; soil ; variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In soil, the distributions of nutrients, water, pores, and microbes vary in time and space. These, in turn, contribute to the variations in root form and function that we see when we grow plants in soil and other non-uniform media. This paper addresses three questions about the consequences of non-uniform distributions of nutrients: how do roots respond to variations in nutrient distribution?; how specific are such responses?; to what extent can we predict them? Roots vary both physiologically and structurally in response to nutrient distributions. The first type of response is primarily a stimulation of nutrient uptake rate per unit of root; the second, a stimulation of root growth where and when the nutrient is most readily available. The first tends to be nutrient-specific and its magnitude related to the extent of the non-uniformity in nutrient availability. The second is less-specific in the sense that the magnitude of the response, when there is one, varies little from one nutrient to another. This leads to apparently exaggerated compensatory capacity, especially for ions that are relatively mobile in soil, such as NO3 -. Because the physiological and molecular mechanisms of these responses are largely unknown, we cannot say how they are co-ordinated within individual plants. Nor can we predict a plant's responses precisely. What we can do is generate statistical descriptions of them by comparing data collected in many experiments for many species. This produces general rules that summarise what happens, but which probably cannot be applied a priori to specific cases. H Lambers Section editor
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-5036
    Keywords: maize ; nitrate ; nitrogen fertilizer ; root zone ; soil solution ; soil solution sampler with looped hollow fiber (LHF-sampler) ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of inorganic nitrogen (N) fertilizer on the ionic composition of the soil solution under maize (Zea mays L.) was studied. A pot experiment was carried out with two treatments combined factorially, with or without N application (Ca(NO3)2; +N and −N treatments, respectively), and with or without plants. Three looped hollow fiber samplers were installed in each pot to sample soil solutions nondestructively from the root zone, seven times during the 50-day growth period. Plants were harvested on the 50th day, and their nutrient contents determined. Effects of N fertilizer on the soil solutions were observed by the first sampling, 2 days after sowing. The concentrations of Ca and NO3 − and electrical conductivity (EC) increased significantly in the +N treatments as direct effects of fertilizer application. In addition, the concentrations of Mg, K, Na and H+ also increased and that of P decreased significantly as indirect effects caused by the re-establishment of chemical equilibria. This suggested the greater supply as well as the greater possibility of leaching loss not only of NO3 − but also of Ca, Mg and K. In the treatments with plants, the concentrations of NO3 −, Ca, Mg and K decreased with time and pH increased significantly compared with the unplanted soil. The depletion of N in the soil solution roughly agreed with the amount of N taken up by the plant. The depletions of K from the soil solution amounted to less than 10% of the amount of the K taken up, suggesting intensive replenishment of K from exchange sites in the soil. Depletions of Ca and Mg were several times higher than the amounts taken up, indicating that the depletions resulted from the adsorption of the divalent cations by the soil rather than uptake by plants. Because NO3 − is hardly absorbed by exchange sites in soil and was the dominant anion in solution, it was concluded that NO3 − had a major role in controlling cation concentrations in the soil solution and, consequently, on their availability for uptake by plants as well as their possible leaching loss. ei]H Marschner
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-0778
    Keywords: GS-NSO cells ; fed-batch cultures ; scale up ; cellular metabolism ; lactate consumption ; recombinant mouse growth hormone ; recombinant rat growth hormone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Investigations of biological effects of prolonged elevation of growth hormone in animals such as mice and rats require large amounts of mouse and rat growth hormone (GH) materials. As an alternative to scarce and expensive pituitary derived materials, both mouse and rat GH were expressed in NSO murine myeloma cells transfected with a vector containing the glutamine synthetase (GS) gene and two copies of mouse or rat GH cDNA. For optimal expression, the mouse GH vector also contained sequences for targeting integration by homologous recombination. Fed-batch culture processes for such clones were developed using a serum-free, glutamine-free medium and scaled up to 250 L production scale reactors. Concentrated solutions of proteins, amino acids and glucose were fed periodically to extend cell growth and culture lifetime, which led to an increase in the maximum viable cell concentration to 3.5×109 cells/L and an up to 10 fold increase in final mouse and rat rGH titers in comparison with batch cultures. For successful scale up, similar culture environmental conditions were maintained at different scales, and specific issues in large scale reactors such as balancing oxygen supply and carbon dioxide removal, were addressed. Very similar cell growth and protein productivity were obtained in the fed-batch cultures at different scales and in different production runs. The final mouse and rat rGH titers were approximately 580 and 240 mg/L, respectively. During fed-batch cultures, the cell growth stage transition was accompanied by a change in cellular metabolism. The specific glucose consumption rate decreased significantly after the transition from the growth to stationary stage, while lactate was produced in the exponential growth stage and became consumed in the stationary stage. This was roughly coincident with the beginning of ammonia and glutamate accumulation at the entry of cells into the stationary stage as the result of a reduced glutamine consumption and periodic nutrient additions.
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  • 9
    Publication Date: 1996-11-01
    Print ISSN: 0040-6090
    Electronic ISSN: 1879-2731
    Topics: Physics
    Published by Elsevier
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  • 10
    Publication Date: 1996-10-01
    Print ISSN: 1360-1385
    Electronic ISSN: 1878-4372
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Cell Press
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