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  • 2005-2009
  • 1995-1999  (20)
  • 1950-1954
  • 1996  (20)
  • 1
    Electronic Resource
    Electronic Resource
    Spectrochromatography of photosynthetic pigments as a fingerprinting technique for microbial phototrophs (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 20 (1996), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: The combination of chromatographic separation using reverse-phase high-performance liquid chromatography and continuous monitoring of the column eluate using a diode-array spectrophotometer allowed qualitative and quantitative pigment profiling of extracts of photosynthetic material in a single run. Carotenoids and the spectrally distinct types of chlorophyll and bacteriochlorophyll can be unambiguously identified even when imperfectly separated on the column. The resulting spectrochromatogram is a fingerprint useful for the rapid characterization of pure cultures or mixed populations. We have developed software to allow recording, manipulation, and presentation of the resulting spectrochromatograms and present results from photosynthetic microbes in pure and mixed cultures. We describe a number of approaches to the presentation of the resulting data in a readily comprehensible form.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1996.tb00306.x
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  • 2
    Electronic Resource
    Electronic Resource
    Membrane inlet mass spectrometric analysis of N-isotope labelling for aquatic denitrification studies (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 20 (1996), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: Techniques are described for measuring the isotope distribution in dissolved nitrate and N2 using membrane inlet mass spectrometry, which allows several gases to be measured in a water sample without the need for any separation steps. The isotope distribution in dissolved nitrate was measured using denitrifying Pseudomonas nautica to reduce the nitrate to N2 which was then measured by mass spectrometry. Pseudomonas nautica NCIMB 1967 was easily grown in nitrate-limited continuous culture minimising intra- or extracellular nitrate or nitrite pools, and the bioassay was tolerant of a range of salinities. The precision of the bioassay when measuring samples with high 15NO3− contents (0.5 μmol) was 0.05 atom%; with 0.1 μmol 15NO3−, the precision was around 0.2 atom%. Differences in labelling of N2 in preserved samples obtained from 15NO3− incubations of water-covered sediment cores were measured on parallel samples with membrane inlet MS and GC-MS. The membrane inlet technique was accurate but the precision on ratio measurements was lower than by GC-MS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1996.tb00309.x
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  • 3
    Electronic Resource
    Electronic Resource
    Applications of impression management and the drama metaphor in marketing: an introduction (1996)
    Bradford : Emerald
    European journal of marketing 30 (1996), S. 6-12 
    Details
    ISSN: 0309-0566
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Economics
    Notes: In recent years, impression management and the application of the metaphor of behaviour as drama have found their way into the marketing literature. While such creative perspectives concerning the marketing enterprise are generally welcome, little effort has been devoted to providing a specific vehicle for their development. This special issue of the European Journal of Marketing represents an attempt to rectify that circumstance. Discusses in broad terms the relationship of impression management to marketing, while arguing that much room for applications such as impression management's drama metaphor abound. To demonstrate the efficacy of impression management for marketing, briefly presents the four articles that comprise this special issue. Finally, registers a call for continued work in the area of impression management's application to marketing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/03090569610130061
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  • 4
    Electronic Resource
    Electronic Resource
    Going green in the service sector: Social responsibility issues, implications and implementation (1996)
    Bradford : Emerald
    European journal of marketing 30 (1996), S. 56-66 
    Details
    ISSN: 0309-0566
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Economics
    Notes: Over the past decade there has been an increasing awareness of the many ways that businesses affect the ecology of the planet. Most of the attention, however, has been directed towards activities of organizations in the manufacturing sector of the economy. Argues that service organizations have social responsibilities in the preservation of the environment, too. Presents the importance to a product, such as longevity or specific marketing, of green practices among services, a framework to describe green activities across the service sector, and a pragmatic means to implement a green programme for service organizations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/03090569610118777
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  • 5
    Electronic Resource
    Electronic Resource
    Tau as a nucleolar protein in human nonneural cells in vitro and in vivo (1996)
    Springer
    Chromosoma 105 (1996), S. 20-30 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The Tau-1 monoclonal antibody was localized to the nucleolus of interphase cells and the nucleolar organizing regions (NORs) of acrocentric chromosomes in cultured human cells. Putative nucleolar and NOR tau was found in HeLa cells and lymphoblasts as well as in nontransformed fibroblasts and lymphocytes. To confirm the presence of tau in the nuclei of these nonneural cells, immunoblotting analysis was performed on isolated nuclei from lymphoblasts. Several tau bands were noted on the blot of the nuclear extract suggesting the presence of multiple tau isoforms. Tau-1 immunostaining demonstrated variable staining intensities between individual acrocentric chromosomes in all cells tested. In cultured peripheral lymphocytes, these staining patterns were the same from one chromosome spread to the next within an individual. This consistency of Tau-1 staining and its variability among NORs was reminiscent of staining patterns obtained using the silver-NOR procedure. Comparisons of Tau-1 immunostaining with silver staining of chromosome spreads from human lymphocytes demonstrated that Tau-1 did not immunostain all of the NORs that were silver stained. The intensity of Tau-1 fluorescence in nucleoli was further shown to be increased in phytohemagglutinin-stimulated lymphocytes, indicating an upregulation of nuclear tau when cells reentered the cell cycle. These results contribute to a growing body of evidence defining tau as a multifunctional protein that may be involved in ribosomal biogenesis and/or rRNA transcription in the nucleus of all cells as well as microtubule-stabilizing functions in the neuronal cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004120050155
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  • 6
    Electronic Resource
    Electronic Resource
    Manipulation of the bacteriochlorophyll c homolog distribution in the green sulfur bacterium Chlorobium tepidum (1996)
    Springer
    Photosynthesis research 48 (1996), S. 385-393 
    Details
    ISSN: 1573-5079
    Keywords: bacteriochlorophyll homologs ; β-cyclodextrin ; chlorosome ; green bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have shown that the green sulfur bacterium Chlorobium tepidum can be grown in batch culture supplemented with potentially toxic fatty alcohols without a major effect on the growth rate if the concentration of the alcohols is kept low either by programmed addition or by adding the alcohol as an inclusion complex with β-cyclodextrin. HPLC and GC analysis of pigment extracts from the supplemented cells showed that the fatty alcohols were incorporated into bacteriochlorophyll c as the esterifying alcohol. It was possible to change up to 43% of the naturally occurring farnesyl ester of bacteriochlorophyll c with the added alcohol. This change in the homolog composition had no effect on the spectral properties of the cells when farnesol was partially replaced by stearol, phytol or geranylgeraniol. However, with dodecanol we obtained a blue-shift of 6 nm of the Qy band of the bacteriochlorophyll c and a concomitant change in the fluorescence emission was observed. The possible significance of these findings is discussed in the light of current ideas about bacteriochlorophyll organization in the chlorosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029471
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  • 7
    Electronic Resource
    Electronic Resource
    Bacteriochlorophyll organization and energy transfer kinetics in chlorosomes from Chloroflexus aurantiacus depend on the light regime during growth (1996)
    Springer
    Photosynthesis research 47 (1996), S. 157-165 
    Details
    ISSN: 1573-5079
    Keywords: circular dichroism ; decay-associated-spectra (DAS) ; fluorescence anisotropy ; green bacteria ; pigment organization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have used measurements of fluorescence and circular dichroism (CD) to compare chlorosome-membrane preparations derived from the green filamentous bacterium Chloroflexus aurantiacus grown in continuous culture at two different light-intensities. The cells grown under low light (6 μmol m−2 s−1) had a higher ratio of bacteriochlorophyll (BChl) c to BChl a than cells grown at a tenfold higher light intensity; the high-light-grown cells had much more carotenoid per bacteriochlorophyll. The anisotropy of the QY band of BChl c was calculated from steady-state fluorescence excitation and emission spectra with polarized light. The results showed that the BChl c in the chlorosomes derived from cells grown under high light has a higher structural order than BChl c in chlorosomes from low-light-grown cells. In the central part of the BChl c fluorescence emission band, the average angles between the transition dipole moments for BChl c molecules and the symmetry axis of the chlorosome rod element were estimated as 25° and 17° in chlorosomes obtained from the low- and high-light-grown cells, respectively. This difference in BChl organization was confirmed by the decay associated spectra of the two samples obtained using picosecond single-photon-counting experiments and global analysis of the fluorescence decays. The shortest decay component obtained, which probably represents energy-transfer from the chlorosome bacteriochlorophylls to the BChl a in the baseplate, was 15 ps in the chlorosomes from high-light-grown cell but only 7 ps in the preparation from low-light grown cells. The CD spectra of the two preparations were very different: chlorosomes from low-light-grown cells had a type II spectrum, while those from high-light-grown cells was of type I (Griebenow et al. (1991) Biochim Biophys Acta 1058: 194–202). The different shapes of the CD spectra confirm the existence of a qualitatively different organization of the BChl c in the two types of chlorosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016178
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  • 8
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    Performance, reliability and uncertainty of total phosphorus models for lakes—II. Stochastic analyses (1996)
    Elsevier
    Details
    Publication Date: 1996-01-01
    Print ISSN: 0043-1354
    Electronic ISSN: 1879-2448
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by Elsevier
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  • 9
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    Performance, reliability and uncertainty of total phosphorus models for lakes—I. Deterministic analyses (1996)
    Elsevier
    Details
    Publication Date: 1996-01-01
    Print ISSN: 0043-1354
    Electronic ISSN: 1879-2448
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by Elsevier
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  • 10
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    Tau as a nucleolar protein in human nonneural cells in vitro and in vivo (1996)
    Springer
    Details
    Publication Date: 1996-07-01
    Print ISSN: 0009-5915
    Electronic ISSN: 1432-0886
    Topics: Biology , Medicine
    Published by Springer
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