Publication Date:
1996-11-29
Description:
A solid phase carbohydrate library was synthesized and screened against Bauhinia purpurea lectin. The library, which contains approximately 1300 di- and trisaccharides, was synthesized with chemical encoding on TentaGel resin so that each bead contained a single carbohydrate. Two ligands that bind more tightly to the lectin than Gal-beta-1,3-GalNAc (the known ligand) have been identified. The strategy outlined can be used to identify carbohydrate-based ligands for any receptor; however, because the derivatized beads mimic the polyvalent presentation of cell surface carbohydrates, the screen may prove especially valuable for discovering new compounds that bind to proteins participating in cell adhesion.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Liang, R -- Yan, L -- Loebach, J -- Ge, M -- Uozumi, Y -- Sekanina, K -- Horan, N -- Gildersleeve, J -- Thompson, C -- Smith, A -- Biswas, K -- Still, W C -- Kahne, D -- New York, N.Y. -- Science. 1996 Nov 29;274(5292):1520-2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry, Princeton University, Princeton, NJ 08544, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8929411" target="_blank"〉PubMed〈/a〉
Keywords:
Acylation
;
Antigens, Tumor-Associated, Carbohydrate/metabolism
;
Carbohydrate Conformation
;
Disaccharides/chemical synthesis/chemistry/metabolism
;
Glycosylation
;
Lectins/*metabolism
;
Ligands
;
Oligosaccharides/*chemical synthesis/chemistry/metabolism
;
*Plant Lectins
;
Polystyrenes
;
Trisaccharides/chemical synthesis/chemistry/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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