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  • 1
    Electronic Resource
    Electronic Resource
    Biological and physicochemical characterization of recombinant human erythropoietins fractionated by Mono Q column chromatography and their modification with sialytransferase (1996)
    Springer
    Glycoconjugate journal 13 (1996), S. 1013-1020 
    Details
    ISSN: 1573-4986
    Keywords: erythropoietin ; BHK cell ; Mono Q column chromatography ; IEF-gel electrophoresis ; pI value ; sialic acid ; oligosaccharide chain ; in vivo activity ; in vitro activity ; α2,6-sialyltransferase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Ten erythropoietin (EPO) fractions differing in sialic acid content, ranging from 9.5 to 13.8 mol mol−1 of EPO, were obtained from baby hamster kidney cell-derived recombinant human EPO by Mono Q column chromatography. The mean pI values of the EPO fractions determined by IEF-gel electrophoresis systematically shifted from 4.11 to 3.31, coinciding with the sialic acid content, without a change in the constitution of asialo N-linked oligosaccharides of each fraction. Although a linear relationship between thein vivo bioactivity and the sialic acid content of the fractionated, samples was observed until 12.1 mol mol−1 of EPO, there was no further increase in their activity over 12.4 mol mol−1 of EPO. On the other hand, an inverse relationship between thein vitro bioactivity and sialic acid content of EPO was observed. Also, we showed that thein vivo bioactivity of some fractions with low sialic acid contents was increased after treatment with α2,6-sialyltransferase, but thein vivo bioactivity of the other fractions with high sialic acid contents was either decreased or not affected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01053197
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  • 2
    Electronic Resource
    Electronic Resource
    Colocation of downy mildew (Plasmopara halstedii) resistance genes in sunflower (Helianthus annuus L.) (1996)
    Springer
    Euphytica 91 (1996), S. 225-228 
    Details
    ISSN: 1573-5060
    Keywords: bulked segregant analysis ; Helianthus annuus ; linkage ; Plasmopara halstedii ; resistance genes ; RFLP ; sunflower ; downy mildew
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The Pl6 locus in the inbred sunflower (Helianthus annuus L.) line HA335 giving resistance to French races of downy mildew (Plasmopara halstedii (Farl.) Berl. & de Toni. was localized by molecular techniques. A bulked segregant analysis was made on the F2 progeny from a cross between this line and H52, a downy mildew susceptible line. The resistance gene in HA335 was found to have the same linked RFLP marker loci as those determined for Pl1 (resistance to race 1 in the line RHA266) on linkage group 1 of the consensus RFLP map of the cultivated sunflower. Pl1 and Pl6 thus appear either to be allelic or closely linked. The implications for sunflower breeding are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021074
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  • 3
    Electronic Resource
    Electronic Resource
    Finite-element analysis of Taylor flow (1996)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 42 (1996), S. 1815-1823 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A finite-element analysis of Taylor flow in a cylindrical capillary was performed using a commercial FEM program (FIDAP) to solve the fundamental fluid dynamics equations together with the capillary forces at the gas-liquid interface. A moving-surface formulation was used to calculate the bubble shape. The thickness of the liquid film surrounding the gas bubble, the degree of mixing in the liquid phase, and the slip velocity between the two phases were calculated. These parameters influence the performance of monolith reactors operating in the Taylor flow regime. On comparison with experimental results it was found that the FEM calculation generally predicts a thinner liquid film, which can possibly be explained in terms of a peripheral variation in surface tension. Moreover, the wavelength of the wiggles predicted in the liquid film near the tail end of the bubble was compared to those arising from a simplified mathematical analysis available in the literature. Good agreement was found for Ca 〈 0.005, while for higher Ca the FEM predicts significantly shorter wavelengths, indicating that the lubrication theory is not valid here.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690420703
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