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  • 1
    Electronic Resource
    Electronic Resource
    Synthesis and reactions of 2-mercaptomethyl-1,3,4-oxadiazolin-5-one (1996)
    Springer
    Monatshefte für Chemie 127 (1996), S. 549-555 
    Details
    ISSN: 1434-4475
    Keywords: 1,3,4-Oxadiazole ; Nitrogen nucleophiles ; Cycloaddition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Die Titelverbindung (4) wurde aus Thioglycolsäurehydrazid und Chlorameisensäureethylester hergestellt. Die Reaktionen von4 mit aromatischen Aminen, Aminoderivaten, Hydrazinhydrat, Hydroxylamin und Formamid sowie die Addition von4 an α,β-ungesättigte Verbindungen (Chalkone, Maleinsäureanhydrid, Chinone, Acetonitril) wurden untersucht.
    Notes: Summary The title compound (4) was synthesized from thioglycolic acid hydrazide and ethyl chloroformate. The reactions of4 with aromatic amines, amino derivatives, hydrazine hydrate, hydroxylamine, and formamide were studied. Addition of compound4 to α,β-unsaturated compounds,i.e. chalcones, maleic anhydride, quinones, and acrylonitrile, was investigated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00807080
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  • 2
    Electronic Resource
    Electronic Resource
    Biological and physicochemical characterization of recombinant human erythropoietins fractionated by Mono Q column chromatography and their modification with sialytransferase (1996)
    Springer
    Glycoconjugate journal 13 (1996), S. 1013-1020 
    Details
    ISSN: 1573-4986
    Keywords: erythropoietin ; BHK cell ; Mono Q column chromatography ; IEF-gel electrophoresis ; pI value ; sialic acid ; oligosaccharide chain ; in vivo activity ; in vitro activity ; α2,6-sialyltransferase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Ten erythropoietin (EPO) fractions differing in sialic acid content, ranging from 9.5 to 13.8 mol mol−1 of EPO, were obtained from baby hamster kidney cell-derived recombinant human EPO by Mono Q column chromatography. The mean pI values of the EPO fractions determined by IEF-gel electrophoresis systematically shifted from 4.11 to 3.31, coinciding with the sialic acid content, without a change in the constitution of asialo N-linked oligosaccharides of each fraction. Although a linear relationship between thein vivo bioactivity and the sialic acid content of the fractionated, samples was observed until 12.1 mol mol−1 of EPO, there was no further increase in their activity over 12.4 mol mol−1 of EPO. On the other hand, an inverse relationship between thein vitro bioactivity and sialic acid content of EPO was observed. Also, we showed that thein vivo bioactivity of some fractions with low sialic acid contents was increased after treatment with α2,6-sialyltransferase, but thein vivo bioactivity of the other fractions with high sialic acid contents was either decreased or not affected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01053197
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  • 3
    Electronic Resource
    Electronic Resource
    Colocation of downy mildew (Plasmopara halstedii) resistance genes in sunflower (Helianthus annuus L.) (1996)
    Springer
    Euphytica 91 (1996), S. 225-228 
    Details
    ISSN: 1573-5060
    Keywords: bulked segregant analysis ; Helianthus annuus ; linkage ; Plasmopara halstedii ; resistance genes ; RFLP ; sunflower ; downy mildew
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The Pl6 locus in the inbred sunflower (Helianthus annuus L.) line HA335 giving resistance to French races of downy mildew (Plasmopara halstedii (Farl.) Berl. & de Toni. was localized by molecular techniques. A bulked segregant analysis was made on the F2 progeny from a cross between this line and H52, a downy mildew susceptible line. The resistance gene in HA335 was found to have the same linked RFLP marker loci as those determined for Pl1 (resistance to race 1 in the line RHA266) on linkage group 1 of the consensus RFLP map of the cultivated sunflower. Pl1 and Pl6 thus appear either to be allelic or closely linked. The implications for sunflower breeding are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021074
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