ISSN:
1573-5117
Schlagwort(e):
aquatic plant media
;
Myriophyllum sibiricum
;
axenic culture
;
pesticide bioassay
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract Myriophyllum sibiricum Komarov, an aquatic dicotyledonous macrophyte with a north temperate distribution, was assessed for use in a new bioassay to determine the effect of pesticides, agricultural runoff and municipal waste upon non-target aquatic macrophytes. An axenic culturing system was developed for which an optimal growth medium is required before a bioassay will be reliable. Five media (Murashige and Skoog, Hoagland's, Gaudet's, modified Andrew's, and Hard Water media), commonly used for aquatic plant culturing, were compared to determine the effect on M. sibiricum growth and development. Morphological endpoints for the assay included shoot length, total root length and number, fresh weight and plant area. Membrane integrity, chlorophyll a, chlorophyll b and carotenoid content were the physiological endpoints examined. Based upon these criteria, the modified Andrew's medium at a pH of 5.8, without the addition of a buffer was chosen as a medium which supported rapid and consistent development of M. sibiricum during the two week assay period.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00012728
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