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Greg, Guldin; Aidan, Southall: Urban Anthropology in China : Leiden, E. J. Brill, 1993 (1995)

Pieke, Frank N.

Cambridge : Cambridge University Press

The @China quarterly 144 (1995), S. 1220-1221

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ISSN: 0305-7410

Quelle: Cambridge Journals Digital Archives

Thema: Allgemeine und vergleichende Sprach- und Literaturwissenschaft. Indogermanistik. Außereuropäische Sprachen und Literaturen , Geschichte , Politikwissenschaft , Sociologie , Wirtschaftswissenschaften

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1017/S0305741000005026

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Digitale Medien

Linear mitochondrial genome organization in vivo in the genus Pythium (1995)

Martin, Frank N.

Springer

Current genetics 28 (1995), S. 225-234

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ISSN: 1432-0983

Schlagwort(e): Pythium ; Linear mitochondrial DNA ; Pulsed-field gel electrophoresis ; ATPase9 ; mtDNA replication

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract Pulsed-field gel electrophoresis (PFGE) of isolates of Pythium oligandrum with linear mitochondrial genomes revealed a distinct band in ethidium bromide-stained gels similar in size to values estimated by restriction mapping of mitochondrial DNA (mtDNA). Southern analysis confirmed that these bands were mtDNA and indicated that linear genomes were present in unit-length size as well as multimers. Isolates of this species with circular mtDNA restriction maps also had low levels of linear mono- and multimers. visualized by Southern analysis of PFGE gels. Examination of 17 additional species revealed similar results; three species had distinct linear mtDNA bands in ethidium bromide-stained gels while the remainder had linear mono- and multi-mers in lower amounts detected only by Southern analysis. Sequence analysis of an isolate of P. oligandrum with a primarily circular mitochondrial genomic map and a low amount of linear molecules revealed that the small unique region of the circular map (which corresponded to the terminal region of linear genomes) was flanked by palindromic intrastrand complementary sequences separated by a unique 194-bp sequence. Sequences with similarity to ATPase9 coding regions from other organisms were located adjacent to this region. Sequences with similarity to mitochondrial origins of replication and autonomously replicating sequences were also located in this region: their potential involvement in the generation of linear molecules is discussed.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00309781

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