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  • Amazonia  (1)
  • Cucumis  (1)
  • Springer  (2)
  • 1990-1994  (2)
  • 1994  (2)
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  • Springer  (2)
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  • 1990-1994  (2)
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  • 1994  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Cusativin, a new cytidine-specific ribonuclease accumulated in seeds of Cucumis sativus L. (1994)
    Springer
    Planta 194 (1994), S. 328-338 
    Details
    ISSN: 1432-2048
    Keywords: Cucumis ; Cusativin ; RNase ; RNA degradation ; Seed (germination maturation)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dry seeds of Cucumis sativus L. were found to contain a heat-sensitive endoribonuclease of a novel type which we have named cusativin. It was purified to apparent electrophoretic homogeneity by chromatography through S-Sepharose Fast Flow, Sephadex G-75, CM-Sepharose, Superdex 75-FPLC (fast protein liquid chromatography) and Mono S-FPLC. It is a single unglycosylated polypeptide chain with an apparent molecular mass (Mr) of 22900. Polyclonal anti-cusativin antibodies raised in rabbits only reacted with melonin, the translation inhibitor from Cucumis melo L. Functional, Western blot and enzyme-linked immunosorbent assay (ELISA) analyses indicated that cusativin is present in the coat and cotyledons of dry seeds, but not in embryonic axes. Cusativin is accumulated in maturing seeds. By contrast, after seed germination there is degradation of the cusativin present in cotyledons but not that present in the seed coat. The preference of cusativin for polynucleotide cleavage was poly(C)≫poly(A) acids, poly(U) and poly(G) being unaffected by cusativin. Under the denaturing conditions used for RNA sequencing, cusativin acted only on poly(C). Cusativin proved to be useful for RNA sequencing, in particular, complementing the data obtained with RNase CL3. Cusativin represents a new class of plant RNase and, as far as we are aware, is the first plant enzyme that shows cleavage specificity for cytidine under the denaturing conditions of RNA sequencing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197532
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Brazilian Amazonia: Industrial development and environmental monitoring (1994)
    Springer
    Environmental management 18 (1994), S. 597-604 
    Details
    ISSN: 1432-1009
    Keywords: Environmental monitoring ; Amazonia ; Remote sensing ; Sustainable development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract After a critical analysis of the strategies for conservation of the environment in Latin America and of large-scale development projects (agriculture and livestock raising, mining and electrical power plants) in the Amazon region of Brazil, this paper deals with the use of high-resolution satellite imagery for environmental monitoring. Two areas were considered in the study, Tucurui and Altamira. Spot/XS and Landsat/TM multispectral images were used, together with color aerial photographs of these areas. A computer-assisted visual interpretation procedure was employed to analyze images, and linear multispectral transformation was applied in selected subscenes in order to improve information extraction. The results indicate that high-resolution satellite imagery is generally compatible with the scale of the environmental problems in Amazonia and is a very efficient source of detailed information at a local scale on many aspects of the environment, in particular for monitoring changes over time in selected areas. Therefore, commercial satellite imagery, processed by specialized software, can accommodate a variety of analysis needs for decision makers and can also be an effective means of verification of international agreements on conservation of the environment in Amazonia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02400862
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    Paper (German National Licenses)
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