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  • biological control
  • Springer  (5)
  • American Chemical Society (ACS)
  • 2015-2019
  • 1990-1994  (5)
  • 1994  (5)
Collection
Publisher
  • Springer  (5)
  • American Chemical Society (ACS)
Years
  • 2015-2019
  • 1990-1994  (5)
Year
  • 1
    ISSN: 1573-8469
    Keywords: biological control ; antibiotics ; Tn5 mutagenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Pseudomonas fluorescens strain CHA0 is an effective biocontrol agent of various soilborne pathogens. It controls damping-off or root rot caused byPythium ultimum on cucumber, wheat and cress. Strain CHA0 synthesizes several antibiotic metabolites such as hydrogen cyanide, 2,4-diacetylphloroglucinol, and pyoluteorin. The role of pyoluteorin in the suppression of damping-off was investigated. Two Tn5 mutants (CHA660 and CHA661) of strain CHA0 were isolated which had lost the capacity to produce pyoluteorin but still produced 2,4-diacteylphloroglucinol and HCN. These mutants still inhibitedP. ultimum on malt agar (which favours the production of 2,4-diacetylphloroglucinol) but had partially lost the ability to inhibit this pathogen on King's B agar (which favours the production of pyoluteorin). The two pyoluteorin-negative mutants showed a reduced capacity to suppress damping-off of cress caused byP. ultimum but were as effective in the protection of cucumber against this pathogen as the wild-type strain. These results indicate that, depending on the plant, pyoluteorin production plays a role in the suppression of damping-off by strain CHA0 without being a major mechanism in disease suppression. We suggest that the contribution of pyoluteorin to the biocontrol activity of strain CHA0 is determined by the quantity of this antibiotic produced in the rhizosphere, which might depend on the root exudates of the host plant.
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  • 2
    ISSN: 1573-8469
    Keywords: Trichoderma spp. ; biological control ; increased growth response ; PGPR ; damping-off
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Trichoderma harzianum was applied to cucumber and pepper seedlings as a peat-bran preparation incorporated into the propagative mixture in a commercial production nursery. On marketing day (after 18 and 30 days for cucumber and pepper, respectively), significant increases of 23.8% and 17.2% in seedling height, 96.1% and 50% in leaf area, and 24.7% and 28.6% in plant dry weight were observed in cucumber and pepper seedlings, respectively, as compared to their non-treated counterparts.Trichoderma-treated seedlings were much more developed and vigorous and had higher chlorophyll contents. No significant differences were found in N, P or K content between treatments. Cucumber seedlings were then transplanted to a commercial greenhouse and analyzed over two successive growth cycles following soil fumigation with methyl bromide (500 kg/ha). Results revealed theTrichoderma-treated plants to be more resistant to damping-off disease. During the first cycle, immediately after soil fumigation, no damping-off was observed with either treatment, except in border beds where 4% of the non-treated plants died, as compared to no damping-off in theTrichoderma-treated plants. During the second growing cycle however, significant reductions in damping-off of 67% and 52% were obtained in middle and border beds, respectively, as compared to the non-treated controls.
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  • 3
    ISSN: 1573-8248
    Keywords: Coccinellidae ; Coleomegilla maculata ; Bacillus thuringiensis ; Leptinotarsa decemlineata ; biological control ; toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé Des bioessais en laboratoire ont été effectués afin de déterminer si les larves de la coccinelle maculée,Coleomegilla maculata lengi Timberlake (Col.: Coccinellidae), sont affectées par M-One™, un insecticide biologique préparé à partir de la bactérieBacillus thuringiensis var.san diego Berliner et utilisé dans la lutte contre le doryphore de la pomme de terre,Leptinotarsa decemlineata (Say) (Col.: Chrysomelidae). Le développement larvaire, effectué sur du pollen traité avec des concentrations de 20 ml M-One™/litre (5,6×108 unités internationales de doryphore/litre) et 200 ml M-One™/litre, a nécessité 29,3 et 38,5 jours respectivement, comparativement à 21,9 jours pour le témoin (eau). M-One™ n'a pas causé de mortalité chez les larves. Au cours d'un test de 48 h, les larves de stade III n'ont montré aucune préférence entre des œufs traités avec 20 ml M-One™/litre et des œufs traités avec de l'eau. Par contre avec 200 ml M-One™/litre, le nombre d'œufs attaqués a diminué significativement de 34,7% par rapport au témoin, 48 h après le début du test. Ces résultats indiquent que l'utilisation de M-One™ à la concentration recommandée de 20 ml/litre ne représente pas une menace pour les populations larvaires de la coccinelle maculée.
    Notes: Abstract Laboratory experiments were conducted to determine the effects of M-One™ (Bacillus thuringiensis var.san diego) on larval instars ofColeomegilla maculata lengi Timberlake. Coccinellid larval development (from egg hatch to adult), completed on pollen treated with suspensions of M-One™ at 20 ml/litre (5.6×108 CPBIU/litre) and 200 ml/litre, took respectively 29.3 and 38.5 days compared with 21.9 days for the control (water). M-One™ did not cause larval mortality.C. maculata third instars did not show any preference between eggs ofLeptinotarsa decemlineata (Say) treated with water or with M-One™ at 20 ml/litre. However, at 200 ml M-One™/litre, the number of eggs attacked was 34.7% lower than the eggs treated with water only, 48 h after the beginning of the test. These results indicate that the use of M-One™, at the manufacturer's recommended field rate of 20 ml/litre, does not cause a major threat to larvalC. maculata populations.
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  • 4
    ISSN: 1573-8248
    Keywords: Podisus maculiventris ; Leptinotarsa decemlineata ; Pieris brassicae ; biological control ; predation rate ; development rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé Des recherches de terrain ont été menées afin d'étudier le degré de prédation journalier et l'effet de la proie sur le développement et la survie des différents stades nymphaux deProdisus maculiventris Say en présence de larves du 3e stade deLeptinotarsa decemlineata (Say) et dePieris brassicae (Linnaeus). Les résultats obtenus montrent que les nymphes du 5e stade et les adultes du prédateur ont une importante activité prédatrice avec un taux de survie élevé quand ils se nourrissent aux dépens de larves du doryphore ou de la Piéride du chou. Par conséquent, il est très probable quePodisus pourrait être utilisé contre ces ravageurs dans des zones où les conditions climatiques sont proches de celles de la région de Thessaloniki (Grèce du Nord).
    Notes: Abstract The mean predation rate per day and the effect of the prey on the development and survival of the various nymphal stages ofPodisus maculiventris (Say) were studied under field-cage conditions. Third instar larvae of the Colorado potato beetleLeptinotarsa decemlineata (Say) and the large white butterflyPieris brassicae (Linnaeus) were used as the prey. The results show that under climatic conditions similar to those occurring in the Thessaloniki region (N. Greece), the 5th instar nymphs and the adults of the predatorP. maculiventris would be promising agents for the biological control of larvae ofL. decemlineata andP. brassicae.
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  • 5
    ISSN: 1573-8248
    Keywords: Dendroctonus micans ; Rhizophagus grandis ; Scolytidae ; Rhizophagidae ; biological control ; mass-rearing ; flight ; take-off ; windtunnel ; quality control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé Le temps passé en conservation à basse température (3–7°C) depuis la métamorphose est intervenu pour 81% dans la perte de la capacité d'envol chez le coléoptère prédateurRhizophagus grandis Gyll. lors d'expériences en tunnel de vol. A l'âge de trois semaines, les insectes sont à leur plus haut niveau d'envol (envol d'environ 80% des insectes). Par la suite, il y a un déclin constant de la capacité d'envol, à raison de 7% des insectes chaque mois. Des changements dans les réserves lipidiques peuvent partiellement expliquer cette réduction, bien qu'il n'y ait pas eu de différence entre le poids frais d'insectes capables de s'envoler et celui d'individus qui en étaient incapables. Le sexe a une influence sur le taux d'envol, avec un taux d'envol significativement plus élevé de 8.7% chez les femelles. La capacité d'envol est encore réduite chez des insectes qui ont été produits dans des élevages de masse où les parents étaient soumis à un mélange de stimuli de ponte de synthèse au lieu d'être mis en présence de larves deD. micans vivantes. Chez les insectes qui prennent leur vol, cependant, la réponse aux attractifs de synthèse est indépendante de l'âge ainsi que des conditions d'élevage.
    Notes: Abstract Time spent by adult beetles in cold storage at 3–7°C accounted for 81% of the loss of take-off capacity inRhizophagus grandis Gyllenhal in windtunnel experiments. At the age of three weeks, the insects were at their highest take-off capacity at about 80%. This was followed by a steady decrease, 7% of the insects failing to take-off each month. Changes in the fat reserves during cold storage could explain at least partly this reduction of flight capacity although there was no significant difference in fresh weight between insects that were able/unable to take-off. Sex had a significant influence on take-off rates, with an 8.7% higher take-off rate in females. Take-off capacity was further reduced when the insects were mass-produced in cultures using parent beetles submitted to a blend of synthetic oviposition stimulants instead of live prey larvae. Response to synthetic attractants by those insects which took-off, however, was not influenced by cold storage or by the use of synthetic stimulants in the culture medium.
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