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  • American Society of Hematology  (2)
  • Cambridge University Press  (1)
  • International Union of Crystallography
  • 2015-2019
  • 2000-2004
  • 1995-1999
  • 1990-1994  (3)
  • 1994  (3)
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  • 2015-2019
  • 2000-2004
  • 1995-1999
  • 1990-1994  (3)
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  • 1
    Publication Date: 1994-06-01
    Description: Sequence analysis of the retinoic acid receptor-alpha (RAR alpha) gene from a subline of HL-60 cells (RA-res) stably resistant to all-trans retinoic acid (RA) disclosed a single-base change in codon number 411, the same C to T transition previously reported in an independently selected HL-60 RA resistant clone by Robertson et al (Blood 80:1885, 1992). This mutation eliminates a FokI restriction endonuclease site. Using primers framing this mutation in exon 9 of the RAR alpha gene, we showed that polymerase chain reaction products amplified from either mRNA or genomic DNA templates from the RA-res subline were completely resistant to FokI digestion whereas those from wild-type (wt) HL-60 cells could be digested to completion. The lack of a normal allele in the RA-res cells was confirmed by mixing experiments and hybridization analyses. Southern blot analysis of DNA from the RA-res and wt cells versus control placental DNA indicated that the RAR alpha gene is not haploid. The independent isolation of the same RAR alpha mutation in different laboratories suggests either that the mutation exits in a small subpopulation in the wt line or that this is a mutational “hot spot.” Furthermore, the results indicate that if a dominant negative mode of resistance is involved in the RA-res subline, this must involve interference with the function of heterologous receptor proteins such as the retinoid X receptors. The lack of any normal RAR alpha in this subline may facilitate studies of the mode of action of retinoids.
    Print ISSN: 0006-4971
    Electronic ISSN: 1528-0020
    Topics: Biology , Medicine
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  • 2
    Publication Date: 1994-06-01
    Description: Sequence analysis of the retinoic acid receptor-alpha (RAR alpha) gene from a subline of HL-60 cells (RA-res) stably resistant to all-trans retinoic acid (RA) disclosed a single-base change in codon number 411, the same C to T transition previously reported in an independently selected HL-60 RA resistant clone by Robertson et al (Blood 80:1885, 1992). This mutation eliminates a FokI restriction endonuclease site. Using primers framing this mutation in exon 9 of the RAR alpha gene, we showed that polymerase chain reaction products amplified from either mRNA or genomic DNA templates from the RA-res subline were completely resistant to FokI digestion whereas those from wild-type (wt) HL-60 cells could be digested to completion. The lack of a normal allele in the RA-res cells was confirmed by mixing experiments and hybridization analyses. Southern blot analysis of DNA from the RA-res and wt cells versus control placental DNA indicated that the RAR alpha gene is not haploid. The independent isolation of the same RAR alpha mutation in different laboratories suggests either that the mutation exits in a small subpopulation in the wt line or that this is a mutational “hot spot.” Furthermore, the results indicate that if a dominant negative mode of resistance is involved in the RA-res subline, this must involve interference with the function of heterologous receptor proteins such as the retinoid X receptors. The lack of any normal RAR alpha in this subline may facilitate studies of the mode of action of retinoids.
    Print ISSN: 0006-4971
    Electronic ISSN: 1528-0020
    Topics: Biology , Medicine
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  • 3
    Publication Date: 1994-09-10
    Description: The linear spin-up problem for a rapidly rotating viscous diffusive ideal gas is considered in the limit of vanishing Ekman number E. Particular attention is given to gases having a large molecular weight. The gas is enclosed in a cylindrical annulus, with flat top and bottom walls, which is rotating around its axis of symmetry with rotation rate Ω. The walls of the container are adiabatic. In a rotating gas (of any molecular weight), the Ekman layers on adiabatic walls are weak, which implies that there is no distinct non-diffusive response of the gas outside the Ekman and Stewartson boundary layers on the timescale E-1/2Ω-1for spin-up of a homogeneous fluid. For the case of adiabatic walls, it is shown that the spin-up mechanisms due to viscous diffusion and Ekman suction are, from a formal point of view, equally strong. Therefore, the gas will adjust to the increased rotation rate of the container on the diffusive timescale E-1 Ω-1. However, if E1/3 ⪡γ — 1 ⪡ 1 and M ~ 1, which characterizes rapidly rotating heavy gases (where y is the ratio of specific heats of the gas and M the Mach number), it is shown that the gas spins up mainly by Ekman suction on the shorter timescale (γ— l)2E-1Ω-1. In such cases, the interior motion splits up into a non-diffusive part of geostrophic character and diffusive boundary layers of thickness (γ — 1) outside the Ekman and Stewartson layers. The motion approaches the steady state of rigid rotation algebraically instead of exponentially as is usually the case for spin-up. © 1994, Cambridge University Press. All rights reserved.
    Print ISSN: 0022-1120
    Electronic ISSN: 1469-7645
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
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