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  • American Association for the Advancement of Science (AAAS)
  • 2015-2019  (3)
  • 1990-1994  (3)
  • 2016  (3)
  • 1994  (3)
  • 1
    Publication Date: 1994-10-21
    Description: The structure of the heterodimeric flavocytochrome c sulfide dehydrogenase from Chromatium vinosum was determined at a resolution of 2.53 angstroms. It contains a glutathione reductase-like flavin-binding subunit and a diheme cytochrome subunit. The diheme cytochrome folds as two domains, each resembling mitochondrial cytochrome c, and has an unusual interpropionic acid linkage joining the two heme groups in the interior of the subunit. The active site of the flavoprotein subunit contains a catalytically important disulfide bridge located above the pyrimidine portion of the flavin ring. A tryptophan, threonine, or tyrosine side chain may provide a partial conduit for electron transfer to one of the heme groups located 10 angstroms from the flavin.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chen, Z W -- Koh, M -- Van Driessche, G -- Van Beeumen, J J -- Bartsch, R G -- Meyer, T E -- Cusanovich, M A -- Mathews, F S -- GM-20530/GM/NIGMS NIH HHS/ -- GM-21277/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1994 Oct 21;266(5184):430-2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7939681" target="_blank"〉PubMed〈/a〉
    Keywords: Binding Sites ; Chromatium/*enzymology ; Computer Graphics ; Crystallography, X-Ray ; Cytochrome c Group/*chemistry ; Electron Transport ; Flavin-Adenine Dinucleotide/metabolism ; Hydrogen Bonding ; Models, Molecular ; Oxidoreductases/*chemistry ; Protein Conformation ; Protein Structure, Secondary
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 2
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1994-06-03
    Description: The plant hormone abscisic acid (ABA) mediates various responses such as stomatal closure, the maintenance of seed dormancy, and the inhibition of plant growth. All three responses are affected in the ABA-insensitive mutant abi1 of Arabidopsis thaliana, suggesting that an early step in the signaling of ABA is controlled by the ABI1 locus. The ABI1 gene was cloned by chromosome walking, and a missense mutation was identified in the structural gene of the abi1 mutant. The ABI1 gene encodes a protein with high similarity to protein serine or threonine phosphatases of type 2C with the novel feature of a putative Ca2+ binding site. Thus, the control of the phosphorylation state of cell signaling components by the ABI1 product could mediate pleiotropic hormone responses.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Meyer, K -- Leube, M P -- Grill, E -- New York, N.Y. -- Science. 1994 Jun 3;264(5164):1452-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Plant Sciences, Swiss Federal Institute of Technology, Zurich.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8197457" target="_blank"〉PubMed〈/a〉
    Keywords: Abscisic Acid/*pharmacology ; Amino Acid Sequence ; Arabidopsis/enzymology/genetics/*metabolism ; *Arabidopsis Proteins ; Binding Sites ; Calcium/metabolism ; Chromosome Walking ; Cloning, Molecular ; Genes, Plant ; Genetic Markers ; Molecular Sequence Data ; Mutation ; Phosphoprotein Phosphatases/chemistry/genetics/*metabolism ; Plants, Genetically Modified ; *Signal Transduction
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
    Publication Date: 1994-12-23
    Description: The tribological properties of C(60) on the mesoscopic scale were investigated with a scanning force microscope, which allowed simultaneous measurements of normal and lateral forces under ultrahigh-vacuum conditions. Islands of C(60), deposited on NaCl(001), could be moved by the action of the probing tip in a controlled way. Different modes of motion, such as translation and rotation, were observed. An extremely small dissipation energy of about 0.25 millielectron volt per molecule and a cohesive energy of 1.5 electron volts were determined in these nanometer-scale experiments. The corresponding shear strength of 0.05 to 0.1 megapascal was smaller by one order of magnitude than typical values of boundary lubricants. For C(60) on graphite, disruption of the islands was observed and collective motion of the islands could not be achieved. These results could find use in the field of nanotechnology; for example, C(60) islands could be developed into a sled-type transport system on the nanometer scale.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Luthi, R -- Meyer, E -- Haefke, H -- Howald, L -- Gutmannsbauer, W -- Guntherodt, H J -- New York, N.Y. -- Science. 1994 Dec 23;266(5193):1979-81.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17836516" target="_blank"〉PubMed〈/a〉
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    Electronic ISSN: 1095-9203
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  • 4
    Publication Date: 2016-02-26
    Description: Antibodies somatically mutate to attain high affinity in germinal centers (GCs). There, competition between B cell clones and among somatic mutants of each clone drives an increase in average affinity across the population. The extent to which higher-affinity cells eliminating competitors restricts clonal diversity is unknown. By combining multiphoton microscopy and sequencing, we show that tens to hundreds of distinct B cell clones seed each GC and that GCs lose clonal diversity at widely disparate rates. Furthermore, efficient affinity maturation can occur in the absence of homogenizing selection, ensuring that many clones can mature in parallel within the same GC. Our findings have implications for development of vaccines in which antibodies with nonimmunodominant specificities must be elicited, as is the case for HIV-1 and influenza.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tas, Jeroen M J -- Mesin, Luka -- Pasqual, Giulia -- Targ, Sasha -- Jacobsen, Johanne T -- Mano, Yasuko M -- Chen, Casie S -- Weill, Jean-Claude -- Reynaud, Claude-Agnes -- Browne, Edward P -- Meyer-Hermann, Michael -- Victora, Gabriel D -- 5DP5OD012146/OD/NIH HHS/ -- S10 OD016326/OD/NIH HHS/ -- New York, N.Y. -- Science. 2016 Mar 4;351(6277):1048-54. doi: 10.1126/science.aad3439. Epub 2016 Feb 18.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA. ; Institut Necker-Enfants Malades, INSERM U1151-CNRS UMR 8253, Sorbonne Paris Cite, Universite Paris Descartes, Faculte de Medecine-Site Broussais, 75014 Paris, France. ; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology (MIT), Cambridge, MA 02142, USA. Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA. ; Department of Systems Immunology and Braunschweig Integrated Centre of Systems Biology, Helmholtz Centre for Infection Research, Inhoffenstrabetae7, 38124 Braunschweig, Germany. Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universitat Braunschweig, Braunschweig, Germany. ; Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA. victora@wi.mit.edu.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26912368" target="_blank"〉PubMed〈/a〉
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    Electronic ISSN: 1095-9203
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  • 5
    Publication Date: 2016-02-27
    Description: The state of vanishing friction known as superlubricity has important applications for energy saving and increasing the lifetime of devices. Superlubricity, as detected with atomic force microscopy, appears when sliding large graphite flakes or gold nanoclusters across surfaces, for example. However, the origin of the behavior is poorly understood because of the lack of a controllable nanocontact. We demonstrated the superlubricity of graphene nanoribbons when sliding on gold with a joint experimental and computational approach. The atomically well-defined contact allows us to trace the origin of superlubricity, unraveling the role played by ribbon size and elasticity, as well as by surface reconstruction. Our results pave the way to the scale-up of superlubricity and thus to the realization of frictionless coatings.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kawai, Shigeki -- Benassi, Andrea -- Gnecco, Enrico -- Sode, Hajo -- Pawlak, Remy -- Feng, Xinliang -- Mullen, Klaus -- Passerone, Daniele -- Pignedoli, Carlo A -- Ruffieux, Pascal -- Fasel, Roman -- Meyer, Ernst -- New York, N.Y. -- Science. 2016 Feb 26;351(6276):957-61. doi: 10.1126/science.aad3569.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physics, University of Basel, Klingelbergstrasse 82, CH-4056 Basel, Switzerland. PRESTO (Precursory Research for Embryonic Science and Technology), Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan. ; nanotech@surfaces Laboratory, Empa, Swiss Federal Laboratories for Materials Science and Technology, Uberlandstrasse 129, 8600 Dubendorf, Switzerland. Institute for Materials Science and Max Bergmann Center of Biomaterials, Technische Universitat Dresden, 01062 Dresden, Germany. ; Instituto Madrileno de Estudios Avanzados en Nanociencia, 28049 Madrid, Spain. Otto Schott Institute of Materials Research, Friedrich Schiller University Jena, 07743 Jena, Germany. ; nanotech@surfaces Laboratory, Empa, Swiss Federal Laboratories for Materials Science and Technology, Uberlandstrasse 129, 8600 Dubendorf, Switzerland. ; Department of Physics, University of Basel, Klingelbergstrasse 82, CH-4056 Basel, Switzerland. ; Department of Chemistry and Food Chemistry, Center for Advancing Electronics Dresden, Technische Universitat Dresden, 01062 Dresden, Germany. ; Max Planck Institute for Polymer Research, 55124 Mainz, Germany. ; nanotech@surfaces Laboratory, Empa, Swiss Federal Laboratories for Materials Science and Technology, Uberlandstrasse 129, 8600 Dubendorf, Switzerland. Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26917767" target="_blank"〉PubMed〈/a〉
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  • 6
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    American Association for the Advancement of Science (AAAS)
    In: Science
    Publication Date: 2016-12-09
    Description: Understanding the conditions that allow speciation to occur is difficult because most research has focused on either long-lived organisms or asexual microorganisms. We propagated bacteriophage λ, a virus with rapid generations and frequent recombination, on two Escherichia coli host genotypes that expressed either the LamB or OmpF receptor. When supplied with either single host (allopatry), phage λ improved its binding to the available receptor while losing its ability to use the alternative. When evolving on both hosts together (sympatry), the viruses split into two lineages with divergent receptor preferences. Although the level of divergence varied among replicates, some lineages evolved reproductive isolation via genetic incompatibilities. This outcome indicates that, under suitable conditions, allopatric and sympatric speciation can occur with similar ease. Authors: Justin R. Meyer, Devin T. Dobias, Sarah J. Medina, Lisa Servilio, Animesh Gupta, Richard E. Lenski
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    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Geosciences , Computer Science , Medicine , Natural Sciences in General , Physics
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